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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001180197                                                           15    9605
NP_036287                                                              15    9605
NP_001180198                                                           15    9605
NP_689622                                                              15    9605
XP_006723189                                                           15    9605
XP_006723188                                                           15    9605
XP_005271947                                                           15    9605
XP_005271946                                                           15    9605
NP_001129058                                                           15    9605
NP_002144                                                              15    9605

>NP_001180197
          Length = 850

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GSTKAAVFS 9
           G TK  +FS
Sbjct: 208 GKTKEVIFS 216



>NP_036287
          Length = 649

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  GSTKAAVFS 9
          G TK  +FS
Sbjct: 7  GKTKEVIFS 15



>NP_001180198
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GSTKAAVFS 9
           G TK  +FS
Sbjct: 154 GKTKEVIFS 162



>NP_689622
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSTKAAVF 8
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>XP_006723189
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GSTKAAVFS 9
           G TK  +FS
Sbjct: 153 GKTKEVIFS 161



>XP_006723188
          Length = 849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   GSTKAAVFS 9
           G TK  +FS
Sbjct: 207 GKTKEVIFS 215



>XP_005271947
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSTKAAVF 8
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>XP_005271946
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSTKAAVF 8
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>NP_001129058
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   GSTKAAVF 8
           G+TKAA F
Sbjct: 111 GTTKAAGF 118



>NP_002144
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GSTKAAV 7
           GS+KAAV
Sbjct: 233 GSSKAAV 239



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.113    0.284 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,407
Number of extensions: 361
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)