Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              20     509
NP_444254                                                              20     509
NP_444253                                                              20     509
NP_444255                                                              20     509
XP_005247551                                                           20     509
XP_005247550                                                           20     509
XP_006713712                                                           20     509
XP_005247549                                                           20     509
NP_056150                                                              17    2528
NP_005112                                                              17    2528
XP_006719370                                                           17    2528
XP_006719369                                                           17    2528
XP_006719368                                                           17    2528
NP_037473                                                              17    4312
NP_945343                                                              17    4312
NP_852478                                                              16    5631
NP_056141                                                              16    5631
NP_001164108                                                           16    5631
NP_001193900                                                           16    5631
NP_002828                                                              16    5631
NP_001103224                                                           16    5631
NP_001193901                                                           16    5631
XP_006719592                                                           16    5631
XP_006719591                                                           16    5631
XP_005277570                                                           16    5631
XP_005277569                                                           16    5631
XP_006711309                                                           16    5631
XP_005277568                                                           16    5631
XP_005277567                                                           16    5631
NP_003283                                                              16    7355
NP_001139334                                                           16    7355
NP_003406                                                              16    7355
NP_001159354                                                           16    7355
NP_001159353                                                           16    7355
NP_775271                                                              16    7355
NP_057029                                                              16    7355
NP_644812                                                              16    7355
NP_001268389                                                           16    7355
NP_001268386                                                           16    7355
NP_722576                                                              16    7355
NP_001268387                                                           16    7355
XP_005262424                                                           16    7355
XP_006723864                                                           16    7355
XP_005260471                                                           16    7355
XP_005245528                                                           16    7355
NP_872418                                                              15    9605
NP_079030                                                              15    9605
NP_689680                                                              15    9605
XP_005257331                                                           15    9605
XP_005257330                                                           15    9605
XP_005252955                                                           15    9605
XP_006717041                                                           15    9605
XP_005248660                                                           15    9605
XP_005248658                                                           15    9605
>NP_444256
          Length = 1794

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1316 RSTSFNVQ 1323
>NP_444254
          Length = 1845

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1316 RSTSFNVQ 1323
>NP_444253
          Length = 1914

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1385 RSTSFNVQ 1392
>NP_444255
          Length = 1863

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1385 RSTSFNVQ 1392
>XP_005247551
          Length = 1855

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1326 RSTSFNVQ 1333
>XP_005247550
          Length = 1856

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1395 RSTSFNVQ 1402
>XP_006713712
          Length = 1923

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1395 RSTSFNVQ 1402
>XP_005247549
          Length = 1924

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RSTSFNVQ 9
            RSTSFNVQ
Sbjct: 1395 RSTSFNVQ 1402
>NP_056150
          Length = 2210

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GRSTSFNVQ 9
            GRST+ N+Q
Sbjct: 1973 GRSTALNMQ 1981
>NP_005112
          Length = 2174

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GRSTSFNVQ 9
            GRST+ N+Q
Sbjct: 1937 GRSTTLNMQ 1945
>XP_006719370
          Length = 2076

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GRSTSFNVQ 9
            GRST+ N+Q
Sbjct: 1839 GRSTALNMQ 1847
>XP_006719369
          Length = 2200

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GRSTSFNVQ 9
            GRST+ N+Q
Sbjct: 1963 GRSTALNMQ 1971
>XP_006719368
          Length = 2209

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    GRSTSFNVQ 9
            GRST+ N+Q
Sbjct: 1972 GRSTALNMQ 1980
>NP_037473
          Length = 396

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  GRSTSFNV 8
          G+ST FNV
Sbjct: 34 GKSTFFNV 41
>NP_945343
          Length = 523

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GRSTSFNVQ 9
           GR+TSF+ Q
Sbjct: 503 GRATSFDSQ 511
>NP_852478
          Length = 1179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RSTSFNVQ 9
           R+T+FNV+
Sbjct: 417 RNTTFNVE 424
>NP_056141
          Length = 1071

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 926 GRSKSFN 932
>NP_001164108
          Length = 1070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 925 GRSKSFN 931
>NP_001193900
          Length = 1907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1235 GRSYQFNVK 1243
>NP_002828
          Length = 1997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1325 GRSYQFNVK 1333
>NP_001103224
          Length = 2215

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1543 GRSYQFNVK 1551
>NP_001193901
          Length = 1907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1235 GRSYQFNVK 1243
>XP_006719592
          Length = 2012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1325 GRSYQFNVK 1333
>XP_006719591
          Length = 2230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    GRSTSFNVQ 9
            GRS  FNV+
Sbjct: 1543 GRSYQFNVK 1551
>XP_005277570
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 773 GRSKSFN 779
>XP_005277569
          Length = 918

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 773 GRSKSFN 779
>XP_006711309
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 926 GRSKSFN 932
>XP_005277568
          Length = 1070

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 925 GRSKSFN 931
>XP_005277567
          Length = 1071

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRSTSFN 7
           GRS SFN
Sbjct: 926 GRSKSFN 932
>NP_003283
          Length = 2363

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    RSTSFNVQ 9
            RSTS NVQ
Sbjct: 1749 RSTSPNVQ 1756
>NP_001139334
          Length = 709

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  GRSTSFNV 8
          GR  SFN+
Sbjct: 5  GRGQSFNI 12
>NP_003406
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSTSFNVQ 9
           G+S SFN Q
Sbjct: 730 GKSFSFNSQ 738
>NP_001159354
          Length = 864

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSTSFNVQ 9
           G+S SFN Q
Sbjct: 647 GKSFSFNSQ 655
>NP_001159353
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRSTSFNVQ 9
           G+S SFN Q
Sbjct: 730 GKSFSFNSQ 738
>NP_775271
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 157 KSTQFNVE 164
>NP_057029
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 157 KSTQFNVE 164
>NP_644812
          Length = 304

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   STSFNVQ 9
           STSF+VQ
Sbjct: 207 STSFDVQ 213
>NP_001268389
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 157 KSTQFNVE 164
>NP_001268386
          Length = 251

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RSTSFNVQ 9
          +ST FNV+
Sbjct: 43 KSTQFNVE 50
>NP_722576
          Length = 3080

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSFNVQ 9
            TSFN+Q
Sbjct: 1761 TSFNIQ 1766
>NP_001268387
          Length = 394

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 186 KSTQFNVE 193
>XP_005262424
          Length = 2875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    TSFNVQ 9
            TSFN+Q
Sbjct: 1556 TSFNIQ 1561
>XP_006723864
          Length = 336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 157 KSTQFNVE 164
>XP_005260471
          Length = 365

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RSTSFNVQ 9
           +ST FNV+
Sbjct: 157 KSTQFNVE 164
>XP_005245528
          Length = 2439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    RSTSFNVQ 9
            RSTS NVQ
Sbjct: 1825 RSTSPNVQ 1832
>NP_872418
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  STSFNVQ 9
          ST+FN+Q
Sbjct: 36 STAFNLQ 42
>NP_079030
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   STSFNV 8
           STSFN+
Sbjct: 165 STSFNI 170
>NP_689680
          Length = 608

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSTSFNVQ 9
           G +T+FNV+
Sbjct: 537 GFTTTFNVE 545
>XP_005257331
          Length = 554

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSTSFNVQ 9
           G +T+FNV+
Sbjct: 483 GFTTTFNVE 491
>XP_005257330
          Length = 648

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRSTSFNVQ 9
           G +T+FNV+
Sbjct: 577 GFTTTFNVE 585
>XP_005252955
          Length = 186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  STSFNVQ 9
          ST+FN+Q
Sbjct: 36 STAFNLQ 42
>XP_006717041
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRSTSFNV 8
           GR T+F+V
Sbjct: 679 GRKTNFSV 686
>XP_005248660
          Length = 236

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  STSFNV 8
          STSFN+
Sbjct: 13 STSFNI 18
>XP_005248658
          Length = 350

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   STSFNV 8
           STSFN+
Sbjct: 165 STSFNI 170
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.121    0.324 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,836
Number of extensions: 684
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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