Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              21     229
NP_001263689                                                           21     229
NP_001119584                                                           21     229
NP_001119586                                                           21     229
NP_001263625                                                           21     229
NP_001119590                                                           21     229
NP_001263690                                                           21     229
NP_001119585                                                           21     229
NP_001263624                                                           21     229
NP_001119588                                                           21     229
NP_001119587                                                           21     229
NP_001119589                                                           21     229
NP_001263627                                                           21     229
NP_001263628                                                           21     229
NP_001263626                                                           21     229
NP_001010892                                                           17    3301
NP_001155136                                                           17    3301
XP_006715532                                                           17    3301
NP_542784                                                              17    3301
NP_001191121                                                           17    4312
NP_001119713                                                           17    4312
NP_001191117                                                           17    4312
NP_001119712                                                           17    4312
NP_001119714                                                           17    4312
NP_005418                                                              17    4312
NP_001191116                                                           17    4312
NP_001191115                                                           17    4312
NP_001191119                                                           17    4312
NP_001191113                                                           17    4312
NP_001191118                                                           17    4312
NP_001191120                                                           17    4312
NP_001191114                                                           17    4312
NP_775297                                                              16    5631
NP_000909                                                              16    5631
XP_005276483                                                           16    5631
XP_005254073                                                           16    5631
XP_005275784                                                           16    5631
NP_001104026                                                           16    7355
NP_001447                                                              16    7355
NP_602297                                                              16    7355
XP_006715049                                                           16    7355
XP_006715048                                                           16    7355
XP_006715047                                                           16    7355
NP_008989                                                              16    7355
NP_877963                                                              15    9605
NP_002651                                                              15    9605
NP_775113                                                              15    9605
NP_689846                                                              15    9605
NP_077320                                                              15    9605
NP_001018123                                                           15    9605
NP_775111                                                              15    9605
NP_775110                                                              15    9605
NP_775112                                                              15    9605
NP_000061                                                              15    9605
NP_001739                                                              15    9605
NP_001139540                                                           15    9605
NP_061748                                                              15    9605
NP_115270                                                              15    9605
XP_005260495                                                           15    9605
XP_006716564                                                           15    9605
XP_005250826                                                           15    9605
XP_005264988                                                           15    9605
XP_005264986                                                           15    9605
XP_005264985                                                           15    9605
XP_006713076                                                           15    9605
NP_001106                                                              15    9605
NP_115269                                                              15    9605
NP_003727                                                              15    9605
>NP_000537
          Length = 393

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 266 GRNSFEVRV 274
>NP_001263689
          Length = 354

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 227 GRNSFEVRV 235
>NP_001119584
          Length = 393

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 266 GRNSFEVRV 274
>NP_001119586
          Length = 341

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 266 GRNSFEVRV 274
>NP_001263625
          Length = 302

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 227 GRNSFEVRV 235
>NP_001119590
          Length = 354

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 227 GRNSFEVRV 235
>NP_001263690
          Length = 354

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 227 GRNSFEVRV 235
>NP_001119585
          Length = 346

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 266 GRNSFEVRV 274
>NP_001263624
          Length = 307

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 227 GRNSFEVRV 235
>NP_001119588
          Length = 209

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 134 GRNSFEVRV 142
>NP_001119587
          Length = 261

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 134 GRNSFEVRV 142
>NP_001119589
          Length = 214

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 134 GRNSFEVRV 142
>NP_001263627
          Length = 182

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 107 GRNSFEVRV 115
>NP_001263628
          Length = 187

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 107 GRNSFEVRV 115
>NP_001263626
          Length = 234

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GRNSFEMRV 9
           GRNSFE+RV
Sbjct: 107 GRNSFEVRV 115
>NP_001010892
          Length = 716

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRNSFE 6
           GRNSFE
Sbjct: 519 GRNSFE 524
>NP_001155136
          Length = 600

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRNSFE 6
           GRNSFE
Sbjct: 519 GRNSFE 524
>XP_006715532
          Length = 632

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GRNSFE 6
           GRNSFE
Sbjct: 519 GRNSFE 524
>NP_542784
          Length = 214

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  GRNSFEMRV 9
          GR+ F MRV
Sbjct: 81 GRDGFHMRV 89
>NP_001191121
          Length = 565

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 215 GRRSFEGRI 223
>NP_001119713
          Length = 450

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_001191117
          Length = 540

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_001119712
          Length = 587

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_001119714
          Length = 426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_005418
          Length = 636

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_001191116
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_001191115
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_001191119
          Length = 506

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_001191113
          Length = 499

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_001191118
          Length = 354

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_001191120
          Length = 491

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 237 GRRSFEGRI 245
>NP_001191114
          Length = 475

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR SFE R+
Sbjct: 286 GRRSFEGRI 294
>NP_775297
          Length = 192

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GRNSFEMRV 9
          G+NSF+ R+
Sbjct: 13 GKNSFKKRL 21
>NP_000909
          Length = 508

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GRN+FE  V
Sbjct: 212 GRNNFEGEV 220
>XP_005276483
          Length = 198

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR S E+RV
Sbjct: 123 GRGSHELRV 131
>XP_005254073
          Length = 198

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR S E+RV
Sbjct: 123 GRGSHELRV 131
>XP_005275784
          Length = 198

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRNSFEMRV 9
           GR S E+RV
Sbjct: 123 GRGSHELRV 131
>NP_001104026
          Length = 2647

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRNSFEMRV 9
           GR+ FE++V
Sbjct: 562 GRSPFEVKV 570
>NP_001447
          Length = 2639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GRNSFEMRV 9
           GR+ FE++V
Sbjct: 562 GRSPFEVKV 570
>NP_602297
          Length = 224

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GRNSFE 6
           GRNSF+
Sbjct: 196 GRNSFQ 201
>XP_006715049
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NSFEMRV 9
           NS+EMR+
Sbjct: 637 NSYEMRL 643
>XP_006715048
          Length = 724

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NSFEMRV 9
           NS+EMR+
Sbjct: 662 NSYEMRL 668
>XP_006715047
          Length = 740

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NSFEMRV 9
           NS+EMR+
Sbjct: 662 NSYEMRL 668
>NP_008989
          Length = 739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NSFEMRV 9
           NS+EMR+
Sbjct: 661 NSYEMRL 667
>NP_877963
          Length = 1290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSFEMRV 9
           N FEMR+
Sbjct: 647 NEFEMRL 653
>NP_002651
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSFEMRV 9
           N FEMR+
Sbjct: 647 NEFEMRL 653
>NP_775113
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NSFEMR 8
          NS+EMR
Sbjct: 78 NSYEMR 83
>NP_689846
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSFEMRV 9
           N FEMR+
Sbjct: 116 NKFEMRL 122
>NP_077320
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 737 NSYEMR 742
>NP_001018123
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSFEMRV 9
           N FEMR+
Sbjct: 116 NKFEMRL 122
>NP_775111
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 231 NSYEMR 236
>NP_775110
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 651 NSYEMR 656
>NP_775112
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3  NSFEMR 8
          NS+EMR
Sbjct: 78 NSYEMR 83
>NP_000061
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 743 NSYEMR 748
>NP_001739
          Length = 700

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 623 NSYEMR 628
>NP_001139540
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   NSFEMR 8
           NS+EMR
Sbjct: 545 NSYEMR 550
>NP_061748
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RNSFEMRV 9
           +NSFE+++
Sbjct: 135 QNSFELQI 142
>NP_115270
          Length = 818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RNSFEMRV 9
           +NSFE+++
Sbjct: 135 QNSFELQI 142
>XP_005260495
          Length = 1330

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSFEMRV 9
           N FEMR+
Sbjct: 647 NEFEMRL 653
>XP_006716564
          Length = 1185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    NSFEMRV 9
            N+FE+R+
Sbjct: 1017 NNFELRI 1023
>XP_005250826
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    NSFEMRV 9
            N+FE+R+
Sbjct: 1053 NNFELRI 1059
>XP_005264988
          Length = 746

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RNSFEM 7
           RN+FEM
Sbjct: 592 RNNFEM 597
>XP_005264986
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RNSFEM 7
           RN+FEM
Sbjct: 579 RNNFEM 584
>XP_005264985
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RNSFEM 7
           RN+FEM
Sbjct: 591 RNNFEM 596
>XP_006713076
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RNSFEM 7
           RN+FEM
Sbjct: 592 RNNFEM 597
>NP_001106
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    NSFEMRV 9
            N+FE+R+
Sbjct: 1083 NNFELRI 1089
>NP_115269
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RNSFEMRV 9
           +NSFE+++
Sbjct: 135 QNSFELQI 142
>NP_003727
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RNSFEMRV 9
           +NSFE+++
Sbjct: 135 QNSFELQI 142
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.137    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 487,146
Number of extensions: 983
Number of successful extensions: 68
Number of sequences better than 10000.0: 68
Number of HSP's gapped: 68
Number of HSP's successfully gapped: 68
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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