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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055592                                                              18    1935
XP_005273417                                                           18    1935
NP_001258017                                                           16    5631
NP_004229                                                              16    5631
NP_001271144                                                           16    5631
NP_001271143                                                           16    5631
NP_005988                                                              16    5631
NP_001258016                                                           16    5631
XP_006712916                                                           16    5631
XP_005247020                                                           16    5631
XP_005247019                                                           16    5631
XP_006712915                                                           16    5631
XP_005247018                                                           16    5631
XP_005247017                                                           16    5631
XP_005247015                                                           16    5631
XP_005247014                                                           16    5631
XP_005247013                                                           16    5631
XP_005247012                                                           16    5631
XP_005247011                                                           16    5631
NP_001530                                                              16    7355
NP_065820                                                              16    7355
NP_079106                                                              16    7355
XP_005251783                                                           16    7355
XP_005248430                                                           16    7355
XP_006710288                                                           16    7355
NP_001155097                                                           16    7355
NP_071426                                                              16    7355
NP_004941                                                              15    9605
NP_001032370                                                           15    9605
NP_443749                                                              15    9605
NP_001269360                                                           15    9605
NP_005728                                                              15    9605

>NP_055592
          Length = 1524

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GRKSIRQKT 9
           GRKS+ Q+T
Sbjct: 711 GRKSLNQRT 719



>XP_005273417
          Length = 1487

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GRKSIRQKT 9
           GRKS+ Q+T
Sbjct: 711 GRKSLNQRT 719



>NP_001258017
          Length = 199

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           RKS+RQ T
Sbjct: 130 RKSMRQST 137



>NP_004229
          Length = 1992

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>NP_001271144
          Length = 2025

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>NP_001271143
          Length = 2040

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>NP_005988
          Length = 364

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           RKS+RQ T
Sbjct: 130 RKSMRQST 137



>NP_001258016
          Length = 375

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           RKS+RQ T
Sbjct: 130 RKSMRQST 137



>XP_006712916
          Length = 1998

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>XP_005247020
          Length = 2013

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>XP_005247019
          Length = 2025

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>XP_006712915
          Length = 2026

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>XP_005247018
          Length = 2026

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 321 RRSTRQKT 328



>XP_005247017
          Length = 2039

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 334 RRSTRQKT 341



>XP_005247015
          Length = 2041

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>XP_005247014
          Length = 2063

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>XP_005247013
          Length = 2067

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>XP_005247012
          Length = 2068

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>XP_005247011
          Length = 2068

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKSIRQKT 9
           R+S RQKT
Sbjct: 363 RRSTRQKT 370



>NP_001530
          Length = 397

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK +R+K
Sbjct: 198 GRKIVREK 205



>NP_065820
          Length = 1388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GRKSIRQ 7
            G+KS+RQ
Sbjct: 1245 GQKSVRQ 1251



>NP_079106
          Length = 534

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK +R+K
Sbjct: 525 GRKPVRKK 532



>XP_005251783
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK IR++
Sbjct: 129 GRKQIRER 136



>XP_005248430
          Length = 486

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK +R+K
Sbjct: 477 GRKPVRKK 484



>XP_006710288
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GRKSIRQKT 9
           G+K  RQKT
Sbjct: 190 GKKPRRQKT 198



>NP_001155097
          Length = 156

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK IR++
Sbjct: 129 GRKQIRER 136



>NP_071426
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   GRKSIRQ 7
           GR SIRQ
Sbjct: 107 GRNSIRQ 113



>NP_004941
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GRKSIRQK 8
           GRK I+Q+
Sbjct: 224 GRKGIKQE 231



>NP_001032370
          Length = 379

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSIRQKT 9
           KS+R+KT
Sbjct: 317 KSLREKT 323



>NP_443749
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSIRQKT 9
           KS+R+KT
Sbjct: 349 KSLREKT 355



>NP_001269360
          Length = 201

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RKSIRQK 8
           RKS++QK
Sbjct: 183 RKSLKQK 189



>NP_005728
          Length = 192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   RKSIRQK 8
           RKS++QK
Sbjct: 183 RKSLKQK 189



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.125    0.320 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 473,743
Number of extensions: 1261
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)