Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000360                                                              19     665
XP_005268094                                                           19     665
NP_000224                                                              18    1482
XP_005264366                                                           18    1482
XP_006712078                                                           18    1482
NP_065394                                                              18    1935
NP_852111                                                              18    1935
NP_000136                                                              18    1935
NP_001159450                                                           16    5631
NP_653308                                                              16    5631
NP_001159449                                                           16    5631
NP_775922                                                              16    5631
XP_005263937                                                           16    5631
XP_005263936                                                           16    5631
NP_001263629                                                           16    7355
NP_612202                                                              16    7355
NP_689942                                                              16    7355
XP_006715441                                                           16    7355
XP_006715440                                                           16    7355
NP_690867                                                              16    7355
NP_055660                                                              15    9605
NP_443099                                                              15    9605
NP_000142                                                              15    9605
NP_002502                                                              15    9605
NP_001245213                                                           15    9605
XP_006721172                                                           15    9605
XP_006720535                                                           15    9605
XP_005254948                                                           15    9605
XP_005254946                                                           15    9605
XP_006720008                                                           15    9605
XP_006720007                                                           15    9605
XP_006720006                                                           15    9605
NP_001138516                                                           15    9605
NP_037404                                                              15    9605
>NP_000360
          Length = 764

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           GMYLLLI S
Sbjct: 464 GMYLLLIAS 472
>XP_005268094
          Length = 764

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           GMYLLLI S
Sbjct: 464 GMYLLLIAS 472
>NP_000224
          Length = 699

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G+YLLLI S
Sbjct: 409 GLYLLLIAS 417
>XP_005264366
          Length = 380

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  GMYLLLITS 9
          G+YLLLI S
Sbjct: 90 GLYLLLIAS 98
>XP_006712078
          Length = 389

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G+YLLLI S
Sbjct: 99  GLYLLLIAS 107
>NP_065394
          Length = 580

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G Y+LL+TS
Sbjct: 353 GWYILLVTS 361
>NP_852111
          Length = 669

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G+YLLLI S
Sbjct: 386 GIYLLLIAS 394
>NP_000136
          Length = 695

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G+YLLLI S
Sbjct: 412 GIYLLLIAS 420
>NP_001159450
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GMYLLLI 7
           G+YLLL+
Sbjct: 114 GLYLLLV 120
>NP_653308
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GMYLLLI 7
           G+YLLL+
Sbjct: 114 GLYLLLV 120
>NP_001159449
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GMYLLLI 7
           G+YLLL+
Sbjct: 114 GLYLLLV 120
>NP_775922
          Length = 6996

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    MYLLLITS 9
            MYL+++TS
Sbjct: 2639 MYLVVVTS 2646
>XP_005263937
          Length = 758

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  GMYLLLI 7
          G+YLLL+
Sbjct: 38 GLYLLLV 44
>XP_005263936
          Length = 834

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GMYLLLI 7
           G+YLLL+
Sbjct: 114 GLYLLLV 120
>NP_001263629
          Length = 891

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MYLLLITS 9
           +YLL ITS
Sbjct: 418 VYLLFITS 425
>NP_612202
          Length = 892

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   MYLLLITS 9
           +YLL ITS
Sbjct: 418 VYLLFITS 425
>NP_689942
          Length = 455

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GMYLLLITS 9
           G  LLLITS
Sbjct: 212 GKILLLITS 220
>XP_006715441
          Length = 316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GMYLLLITS 9
           G  LLLITS
Sbjct: 212 GKILLLITS 220
>XP_006715440
          Length = 337

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GMYLLLITS 9
           G  LLLITS
Sbjct: 212 GKILLLITS 220
>NP_690867
          Length = 674

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   GMYLLLITS 9
           G  LLLITS
Sbjct: 565 GQKLLLITS 573
>NP_055660
          Length = 907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GMYLLLIT 8
           G Y++LIT
Sbjct: 104 GYYIVLIT 111
>NP_443099
          Length = 1738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GMYLLLI 7
            GM+LLL+
Sbjct: 1341 GMFLLLL 1347
>NP_000142
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   GMYLLLITS 9
           G+Y +++TS
Sbjct: 125 GVYYVMVTS 133
>NP_002502
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  MYLLLIT 8
          +YLL+IT
Sbjct: 49 LYLLIIT 55
>NP_001245213
          Length = 312

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   MYLLLIT 8
           MY+L+IT
Sbjct: 139 MYVLMIT 145
>XP_006721172
          Length = 139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  GMYLLLI 7
          G+YLLL+
Sbjct: 33 GIYLLLL 39
>XP_006720535
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MYLLLI 7
           MYLLLI
Sbjct: 176 MYLLLI 181
>XP_005254948
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  MYLLLI 7
          MYLLLI
Sbjct: 61 MYLLLI 66
>XP_005254946
          Length = 697

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MYLLLI 7
           MYLLLI
Sbjct: 176 MYLLLI 181
>XP_006720008
          Length = 1645

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GMYLLLI 7
            GM+LLL+
Sbjct: 1248 GMFLLLL 1254
>XP_006720007
          Length = 1709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GMYLLLI 7
            GM+LLL+
Sbjct: 1312 GMFLLLL 1318
>XP_006720006
          Length = 1713

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    GMYLLLI 7
            GM+LLL+
Sbjct: 1316 GMFLLLL 1322
>NP_001138516
          Length = 692

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MYLLLI 7
           MYLLLI
Sbjct: 176 MYLLLI 181
>NP_037404
          Length = 710

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   MYLLLI 7
           MYLLLI
Sbjct: 176 MYLLLI 181
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.150    0.407 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,950
Number of extensions: 810
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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