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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              18    1935
NP_001549                                                              17    4312
XP_006718896                                                           17    4312
NP_005561                                                              16    5631
NP_037514                                                              16    7355
NP_060555                                                              16    7355
NP_000806                                                              16    7355
NP_542183                                                              16    7355
NP_001243727                                                           16    7355
XP_005260347                                                           16    7355
XP_005260346                                                           16    7355
XP_005260345                                                           16    7355
XP_005260343                                                           16    7355
XP_005260341                                                           16    7355
XP_006723766                                                           16    7355
XP_006723765                                                           16    7355
XP_005260340                                                           16    7355
XP_005260339                                                           16    7355
XP_005260342                                                           16    7355
XP_006711503                                                           16    7355
NP_001269137                                                           15    9605
NP_001273501                                                           15    9605
NP_689491                                                              15    9605
NP_055586                                                              15    9605
NP_001193962                                                           15    9605
NP_001193963                                                           15    9605
NP_075055                                                              15    9605
XP_005260077                                                           15    9605
XP_005268355                                                           15    9605
XP_005266922                                                           15    9605
NP_001025029                                                           15    9605
NP_036316                                                              15    9605
NP_057079                                                              15    9605

>NP_055365
          Length = 1032

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   GLRSRIRAI 9
           GL SRIRAI
Sbjct: 903 GLGSRIRAI 911



>NP_001549
          Length = 578

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GLRSRIRAI 9
           G R+R+RA+
Sbjct: 95  GYRARVRAV 103



>XP_006718896
          Length = 558

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLRSRIRAI 9
          G R+R+RA+
Sbjct: 75 GYRARVRAV 83



>NP_005561
          Length = 510

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLRSRIRAI 9
          GLR+R+R +
Sbjct: 8  GLRARVRPL 16



>NP_037514
          Length = 750

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LRSRIRAI 9
           LRSRIR I
Sbjct: 470 LRSRIRFI 477



>NP_060555
          Length = 1041

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GLRSRIRAI 9
           G+R+R RA+
Sbjct: 204 GIRTRSRAV 212



>NP_000806
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   GLRSRIRAI 9
           G+R+R+R I
Sbjct: 414 GIRARLRPI 422



>NP_542183
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 115 GLRSRVK 121



>NP_001243727
          Length = 680

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 359 GLRSRVK 365



>XP_005260347
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 115 GLRSRVK 121



>XP_005260346
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 115 GLRSRVK 121



>XP_005260345
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 115 GLRSRVK 121



>XP_005260343
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_005260341
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_006723766
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_006723765
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_005260340
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_005260339
          Length = 545

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 224 GLRSRVK 230



>XP_005260342
          Length = 602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLRSRIR 7
           GLRSR++
Sbjct: 281 GLRSRVK 287



>XP_006711503
          Length = 860

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  GLRSRIRAI 9
          G+R+R RA+
Sbjct: 23 GIRTRSRAV 31



>NP_001269137
          Length = 159

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   GLRSRI 6
           GLRSRI
Sbjct: 129 GLRSRI 134



>NP_001273501
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRSRIRAI 9
           LRSRIR +
Sbjct: 703 LRSRIREL 710



>NP_689491
          Length = 100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GLRSRIRAI 9
          GLR + RA+
Sbjct: 78 GLRKKFRAL 86



>NP_055586
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRSRIRAI 9
           LRSRIR +
Sbjct: 703 LRSRIREL 710



>NP_001193962
          Length = 1000

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRSRIRAI 9
           LRSRIR +
Sbjct: 703 LRSRIREL 710



>NP_001193963
          Length = 901

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LRSRIRAI 9
           LRSRIR +
Sbjct: 603 LRSRIREL 610



>NP_075055
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LRSRIRA 8
           LR+RIRA
Sbjct: 415 LRARIRA 421



>XP_005260077
          Length = 1005

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LRSRIRA 8
           LR+RIRA
Sbjct: 409 LRARIRA 415



>XP_005268355
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   GLRSRIRAI 9
           G RSR RA+
Sbjct: 288 GTRSRRRAV 296



>XP_005266922
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GLRSRIRAI 9
          G+R RIR +
Sbjct: 47 GVRQRIRKV 55



>NP_001025029
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  GLRSRIRAI 9
          G+R RIR +
Sbjct: 47 GVRQRIRKV 55



>NP_036316
          Length = 408

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GLRSRIRA 8
           GLR R+R+
Sbjct: 375 GLRQRLRS 382



>NP_057079
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GLRSRIR 7
           GLR R+R
Sbjct: 146 GLRQRVR 152



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.340    0.152    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,187
Number of extensions: 513
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)