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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_060050                                                              16    5631
XP_005269984                                                           16    5631
XP_005269983                                                           16    5631
XP_005269982                                                           16    5631
XP_006717970                                                           16    5631
NP_000008                                                              16    7355

>NP_060050
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GVMVAMQ 9
           GV+VAMQ
Sbjct: 659 GVLVAMQ 665



>XP_005269984
          Length = 382

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GVMVAMQ 9
           GV+VAMQ
Sbjct: 333 GVLVAMQ 339



>XP_005269983
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GVMVAMQ 9
           GV+VAMQ
Sbjct: 659 GVLVAMQ 665



>XP_005269982
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GVMVAMQ 9
           GV+VAMQ
Sbjct: 685 GVLVAMQ 691



>XP_006717970
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GVMVAMQ 9
           GV+VAMQ
Sbjct: 685 GVLVAMQ 691



>NP_000008
          Length = 412

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   GLGVMVAMQ 9
           G+G  +AMQ
Sbjct: 258 GMGFKIAMQ 266



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.141    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 299,442
Number of extensions: 231
Number of successful extensions: 6
Number of sequences better than 10000.0: 6
Number of HSP's gapped: 6
Number of HSP's successfully gapped: 6
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)