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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              17    2528
NP_671714                                                              17    2528
XP_006715890                                                           17    2528
XP_005250160                                                           17    2528
XP_005250159                                                           17    2528
NP_001073960                                                           16    7355
NP_055503                                                              16    7355
NP_001193620                                                           16    7355
NP_998759                                                              16    7355
XP_006717605                                                           16    7355
NP_115880                                                              16    7355
NP_000375                                                              15    9605
NP_066990                                                              15    9605

>NP_005742
          Length = 3907

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLAIKESVA 9
            GLAIKES A
Sbjct: 2262 GLAIKESDA 2270



>NP_671714
          Length = 3899

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLAIKESVA 9
            GLAIKES A
Sbjct: 2254 GLAIKESDA 2262



>XP_006715890
          Length = 3860

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLAIKESVA 9
            GLAIKES A
Sbjct: 2215 GLAIKESDA 2223



>XP_005250160
          Length = 3939

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLAIKESVA 9
            GLAIKES A
Sbjct: 2294 GLAIKESDA 2302



>XP_005250159
          Length = 3952

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    GLAIKESVA 9
            GLAIKES A
Sbjct: 2307 GLAIKESDA 2315



>NP_001073960
          Length = 845

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GLAIKESV 8
           GL+I+ESV
Sbjct: 836 GLSIQESV 843



>NP_055503
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GLAIKESV 8
           GL+I+ESV
Sbjct: 819 GLSIQESV 826



>NP_001193620
          Length = 744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLAIKES 7
           G+A+KES
Sbjct: 140 GIAVKES 146



>NP_998759
          Length = 735

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   GLAIKES 7
           G+A+KES
Sbjct: 131 GIAVKES 137



>XP_006717605
          Length = 856

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GLAIKESV 8
           GL+I+ESV
Sbjct: 847 GLSIQESV 854



>NP_115880
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  GLAIKES 7
          G+A+KES
Sbjct: 37 GIAVKES 43



>NP_000375
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    LAIKESV 8
            LA+KESV
Sbjct: 2822 LALKESV 2828



>NP_066990
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LAIKESVA 9
           + IKESVA
Sbjct: 259 ITIKESVA 266



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.126    0.297 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 340,941
Number of extensions: 333
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)