Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              21     175
NP_001263689                                                           21     175
NP_001119584                                                           21     175
NP_001119590                                                           21     175
NP_001263690                                                           21     175
NP_001119587                                                           21     175
NP_001263626                                                           21     175
NP_001119585                                                           18    1482
NP_001263624                                                           18    1482
NP_001119589                                                           18    1482
NP_001263628                                                           18    1482
NP_001119586                                                           18    1935
NP_001263625                                                           18    1935
NP_001119588                                                           18    1935
NP_001263627                                                           18    1935
NP_997196                                                              17    3301
XP_006721755                                                           17    3301
NP_066000                                                              17    4312
NP_002579                                                              17    4312
NP_115778                                                              17    4312
XP_006713781                                                           17    4312
NP_004645                                                              16    5631
NP_001269649                                                           16    5631
NP_001269650                                                           16    5631
NP_001269648                                                           16    5631
NP_068744                                                              16    5631
NP_001269651                                                           16    5631
NP_001269652                                                           16    5631
NP_001269653                                                           16    5631
NP_001034680                                                           16    5631
NP_001034679                                                           16    5631
NP_008849                                                              16    5631
NP_848928                                                              16    5631
XP_005272733                                                           16    5631
XP_005272732                                                           16    5631
NP_001020127                                                           16    5631
NP_001037729                                                           16    7355
NP_573403                                                              16    7355
NP_877496                                                              16    7355
XP_006723917                                                           16    7355
XP_005260553                                                           16    7355
NP_001003787                                                           15    9605
NP_699166                                                              15    9605
NP_115515                                                              15    9605
NP_001035100                                                           15    9605
NP_001159442                                                           15    9605
NP_001159441                                                           15    9605
NP_001003788                                                           15    9605
NP_001003786                                                           15    9605
XP_005259367                                                           15    9605
XP_006723476                                                           15    9605
XP_006723475                                                           15    9605
XP_005257860                                                           15    9605
XP_005257858                                                           15    9605
XP_005257857                                                           15    9605
XP_005257856                                                           15    9605
XP_005257855                                                           15    9605
XP_005257854                                                           15    9605
XP_006720934                                                           15    9605
>NP_000537
          Length = 393

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 325 GEYFTLQIR 333
>NP_001263689
          Length = 354

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 286 GEYFTLQIR 294
>NP_001119584
          Length = 393

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 325 GEYFTLQIR 333
>NP_001119590
          Length = 354

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 286 GEYFTLQIR 294
>NP_001263690
          Length = 354

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 286 GEYFTLQIR 294
>NP_001119587
          Length = 261

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 193 GEYFTLQIR 201
>NP_001263626
          Length = 234

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   GEYFTLRIR 9
           GEYFTL+IR
Sbjct: 166 GEYFTLQIR 174
>NP_001119585
          Length = 346

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GEYFTLRI 8
           GEYFTL++
Sbjct: 325 GEYFTLQM 332
>NP_001263624
          Length = 307

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GEYFTLRI 8
           GEYFTL++
Sbjct: 286 GEYFTLQM 293
>NP_001119589
          Length = 214

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GEYFTLRI 8
           GEYFTL++
Sbjct: 193 GEYFTLQM 200
>NP_001263628
          Length = 187

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   GEYFTLRI 8
           GEYFTL++
Sbjct: 166 GEYFTLQM 173
>NP_001119586
          Length = 341

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GEYFTLR 7
           GEYFTL+
Sbjct: 325 GEYFTLQ 331
>NP_001263625
          Length = 302

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GEYFTLR 7
           GEYFTL+
Sbjct: 286 GEYFTLQ 292
>NP_001119588
          Length = 209

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GEYFTLR 7
           GEYFTL+
Sbjct: 193 GEYFTLQ 199
>NP_001263627
          Length = 182

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   GEYFTLR 7
           GEYFTL+
Sbjct: 166 GEYFTLQ 172
>NP_997196
          Length = 984

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EYFTLRIR 9
           E+FTLR++
Sbjct: 238 EHFTLRVK 245
>XP_006721755
          Length = 978

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   EYFTLRIR 9
           E+FTLR++
Sbjct: 232 EHFTLRVK 239
>NP_066000
          Length = 771

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YFTLRIR 9
           Y+T+RIR
Sbjct: 541 YYTMRIR 547
>NP_002579
          Length = 934

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EYF LR++
Sbjct: 178 EYFALRVQ 185
>NP_115778
          Length = 863

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EYF LR++
Sbjct: 178 EYFALRVQ 185
>XP_006713781
          Length = 771

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   YFTLRIR 9
           Y+T+RIR
Sbjct: 541 YYTMRIR 547
>NP_004645
          Length = 2555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GEYFTL 6
            G+YFTL
Sbjct: 1375 GDYFTL 1380
>NP_001269649
          Length = 277

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFTL++
Sbjct: 93  GSYFTLQM 100
>NP_001269650
          Length = 195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GEYFTLRI 8
          G YFTL++
Sbjct: 11 GSYFTLQM 18
>NP_001269648
          Length = 283

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFTL++
Sbjct: 99  GSYFTLQM 106
>NP_068744
          Length = 325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFTL++
Sbjct: 141 GSYFTLQM 148
>NP_001269651
          Length = 195

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  GEYFTLRI 8
          G YFTL++
Sbjct: 11 GSYFTLQM 18
>NP_001269652
          Length = 166

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFTL++
Sbjct: 141 GSYFTLQM 148
>NP_001269653
          Length = 124

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFTL++
Sbjct: 99  GSYFTLQM 106
>NP_001034680
          Length = 2554

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GEYFTL 6
            G+YFTL
Sbjct: 1373 GDYFTL 1378
>NP_001034679
          Length = 2570

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GEYFTL 6
            G+YFTL
Sbjct: 1373 GDYFTL 1378
>NP_008849
          Length = 299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GEYFTL 6
           G+YFTL
Sbjct: 241 GQYFTL 246
>NP_848928
          Length = 197

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   GEYFTLRI 8
           G YFT+++
Sbjct: 134 GTYFTMKV 141
>XP_005272733
          Length = 2559

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GEYFTL 6
            G+YFTL
Sbjct: 1378 GDYFTL 1383
>XP_005272732
          Length = 2575

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    GEYFTL 6
            G+YFTL
Sbjct: 1378 GDYFTL 1383
>NP_001020127
          Length = 299

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   GEYFTL 6
           G+YFTL
Sbjct: 241 GQYFTL 246
>NP_001037729
          Length = 139

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  GEYFTL 6
          G+YFTL
Sbjct: 50 GKYFTL 55
>NP_573403
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  EYFTLR 7
          EY+TLR
Sbjct: 47 EYYTLR 52
>NP_877496
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  EYFTLR 7
          EY+TLR
Sbjct: 47 EYYTLR 52
>XP_006723917
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  EYFTLR 7
          EY+TLR
Sbjct: 47 EYYTLR 52
>XP_005260553
          Length = 720

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2  EYFTLR 7
          EY+TLR
Sbjct: 47 EYYTLR 52
>NP_001003787
          Length = 431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GEYFTLR 7
           GEY T+R
Sbjct: 94  GEYVTVR 100
>NP_699166
          Length = 348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 57 GEYVTVR 63
>NP_115515
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EY T++IR
Sbjct: 913 EYVTVKIR 920
>NP_001035100
          Length = 235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FTLRIR 9
          FTLR+R
Sbjct: 45 FTLRVR 50
>NP_001159442
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 50 GEYVTVR 56
>NP_001159441
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 65 GEYVTVR 71
>NP_001003788
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 36 GEYVTVR 42
>NP_001003786
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 57 GEYVTVR 63
>XP_005259367
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EY T++IR
Sbjct: 717 EYVTVKIR 724
>XP_006723476
          Length = 979

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EY T++IR
Sbjct: 913 EYVTVKIR 920
>XP_006723475
          Length = 1050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   EYFTLRIR 9
           EY T++IR
Sbjct: 913 EYVTVKIR 920
>XP_005257860
          Length = 234

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GEYFTLR 7
           GEY T+R
Sbjct: 94  GEYVTVR 100
>XP_005257858
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GEYFTLR 7
           GEY T+R
Sbjct: 94  GEYVTVR 100
>XP_005257857
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   GEYFTLR 7
           GEY T+R
Sbjct: 94  GEYVTVR 100
>XP_005257856
          Length = 373

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 36 GEYVTVR 42
>XP_005257855
          Length = 402

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 65 GEYVTVR 71
>XP_005257854
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  GEYFTLR 7
          GEY T+R
Sbjct: 86 GEYVTVR 92
>XP_006720934
          Length = 296

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  FTLRIR 9
          FTLR+R
Sbjct: 29 FTLRVR 34
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.158    0.477 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 464,123
Number of extensions: 549
Number of successful extensions: 59
Number of sequences better than 10000.0: 59
Number of HSP's gapped: 59
Number of HSP's successfully gapped: 59
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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