Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     509
XP_006713721                                                           20     509
NP_001265249                                                           17    3301
NP_001094379                                                           17    3301
NP_001012417                                                           17    3301
NP_004858                                                              17    4312
NP_001165052                                                           17    4312
NP_002477                                                              17    4312
XP_005252054                                                           17    4312
NP_036529                                                              16    5631
XP_005266764                                                           16    5631
XP_005266762                                                           16    5631
NP_001124388                                                           16    7355
NP_940980                                                              16    7355
NP_004603                                                              16    7355
NP_000646                                                              16    7355
XP_005268686                                                           16    7355
XP_005252207                                                           16    7355
NP_006260                                                              15    9605
NP_110400                                                              15    9605
NP_001001671                                                           15    9605
XP_006717224                                                           15    9605
XP_005266150                                                           15    9605
XP_006717221                                                           15    9605
XP_006717220                                                           15    9605
XP_006717219                                                           15    9605
XP_006717216                                                           15    9605
XP_006717215                                                           15    9605
XP_006717214                                                           15    9605

>NP_006209
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  FVSVTEEAE 9
          FVSVT+EAE
Sbjct: 70 FVSVTQEAE 78



>XP_006713721
          Length = 1068

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  FVSVTEEAE 9
          FVSVT+EAE
Sbjct: 70 FVSVTQEAE 78



>NP_001265249
          Length = 93

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3  SVTEEAE 9
          SVTEEAE
Sbjct: 84 SVTEEAE 90



>NP_001094379
          Length = 112

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SVTEEAE 9
           SVTEEAE
Sbjct: 103 SVTEEAE 109



>NP_001012417
          Length = 152

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   SVTEEAE 9
           SVTEEAE
Sbjct: 143 SVTEEAE 149



>NP_004858
          Length = 263

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FVSVTEEAE 9
           F+ VTEEA+
Sbjct: 108 FLPVTEEAD 116



>NP_001165052
          Length = 219

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FVSVTEEAE 9
          F+ VTEEA+
Sbjct: 64 FLPVTEEAD 72



>NP_002477
          Length = 790

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FVSVTEEAE 9
           FVSVT+E +
Sbjct: 152 FVSVTQEED 160



>XP_005252054
          Length = 723

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  FVSVTEEAE 9
          FVSVT+E +
Sbjct: 85 FVSVTQEED 93



>NP_036529
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FVSVTEE 7
           FV+VTEE
Sbjct: 335 FVAVTEE 341



>XP_005266764
          Length = 255

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FVSVTEE 7
           FV+VTEE
Sbjct: 199 FVAVTEE 205



>XP_005266762
          Length = 400

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FVSVTEE 7
           FV+VTEE
Sbjct: 344 FVAVTEE 350



>NP_001124388
          Length = 426

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FVSVTEEAE 9
          FVSVTE  +
Sbjct: 55 FVSVTETTD 63



>NP_940980
          Length = 2527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSVTEEAE 9
            FV+ TEE++
Sbjct: 1487 FVNATEESD 1495



>NP_004603
          Length = 503

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FVSVTEEAE 9
          FVSVTE  +
Sbjct: 55 FVSVTETTD 63



>NP_000646
          Length = 385

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SVTEEAE 9
           S+TEEAE
Sbjct: 119 SLTEEAE 125



>XP_005268686
          Length = 2521

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    FVSVTEEAE 9
            FV+ TEE++
Sbjct: 1487 FVNATEESD 1495



>XP_005252207
          Length = 507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FVSVTEEAE 9
          FVSVTE  +
Sbjct: 55 FVSVTETTD 63



>NP_006260
          Length = 2156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    VSVTEEAE 9
            +++TEEA+
Sbjct: 1170 IAITEEAD 1177



>NP_110400
          Length = 451

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VTEEAE 9
           VTEEAE
Sbjct: 438 VTEEAE 443



>NP_001001671
          Length = 1313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FVSVTEEAE 9
           +VS+  EAE
Sbjct: 544 YVSINNEAE 552



>XP_006717224
          Length = 915

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_005266150
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717221
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717220
          Length = 825

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717219
          Length = 856

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717216
          Length = 916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717215
          Length = 928

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



>XP_006717214
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FVSVTEEA 8
          FVS+ EEA
Sbjct: 90 FVSLREEA 97



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.115    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,346
Number of extensions: 486
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)