Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_071319                                                              18    1482
NP_061123                                                              18    1482
XP_005257571                                                           18    1482
XP_005257570                                                           18    1482
XP_005257569                                                           18    1482
NP_060051                                                              17    3301
NP_000360                                                              17    3301
XP_005268094                                                           17    3301
NP_001248770                                                           16    5631
XP_005255642                                                           16    5631
NP_001098718                                                           16    5631
NP_079056                                                              16    5631
NP_536724                                                              16    7355
NP_001026851                                                           15    9605
NP_004928                                                              15    9605
NP_071351                                                              15    9605
NP_061722                                                              15    9605
XP_006721527                                                           15    9605
XP_006721526                                                           15    9605
XP_005256542                                                           15    9605
NP_114089                                                              15    9605
>NP_071319
          Length = 486

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           FVLT+I+VA
Sbjct: 110 FVLTIILVA 118
>NP_061123
          Length = 453

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  FVLTLIIVA 9
          FVLT+I+VA
Sbjct: 77 FVLTIILVA 85
>XP_005257571
          Length = 496

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           FVLT+I+VA
Sbjct: 110 FVLTIILVA 118
>XP_005257570
          Length = 542

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           FVLT+I+VA
Sbjct: 165 FVLTIILVA 173
>XP_005257569
          Length = 551

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           FVLT+I+VA
Sbjct: 165 FVLTIILVA 173
>NP_060051
          Length = 479

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           FV+T++I+A
Sbjct: 468 FVMTILIIA 476
>NP_000360
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FVLTLIIVA 9
           FVLTL IVA
Sbjct: 585 FVLTLNIVA 593
>XP_005268094
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   FVLTLIIVA 9
           FVLTL IVA
Sbjct: 585 FVLTLNIVA 593
>NP_001248770
          Length = 1006

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FVLTLIIV 8
           F+LTLI++
Sbjct: 909 FILTLIVL 916
>XP_005255642
          Length = 647

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FVLTLIIV 8
           F+LTLI++
Sbjct: 550 FILTLIVL 557
>NP_001098718
          Length = 1006

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FVLTLIIV 8
           F+LTLI++
Sbjct: 909 FILTLIVL 916
>NP_079056
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FVLTLIIV 8
           F+LTLI++
Sbjct: 663 FILTLIVL 670
>NP_536724
          Length = 435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FVLTLIIV 8
           FVLT II+
Sbjct: 356 FVLTCIII 363
>NP_001026851
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLTLIIV 8
           VLTLII+
Sbjct: 214 VLTLIII 220
>NP_004928
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLTLIIV 8
           VLTLII+
Sbjct: 214 VLTLIII 220
>NP_071351
          Length = 2752

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FVLTLIIV 8
            FVLT+I +
Sbjct: 1300 FVLTIIFI 1307
>NP_061722
          Length = 1007

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           F+LT+II++
Sbjct: 719 FLLTIIILS 727
>XP_006721527
          Length = 253

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  VLTLIIV 8
          VLTLII+
Sbjct: 67 VLTLIII 73
>XP_006721526
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLTLIIV 8
           VLTLII+
Sbjct: 214 VLTLIII 220
>XP_005256542
          Length = 400

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLTLIIV 8
           VLTLII+
Sbjct: 214 VLTLIII 220
>NP_114089
          Length = 884

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   FVLTLIIVA 9
           F+LT+II++
Sbjct: 719 FLLTIIILS 727
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.356    0.166    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 272,172
Number of extensions: 464
Number of successful extensions: 21
Number of sequences better than 10000.0: 21
Number of HSP's gapped: 21
Number of HSP's successfully gapped: 21
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (17.0 bits)
S2: 28 (15.4 bits)

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