Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001257310                                                           17    3301
XP_005273823                                                           17    3301
NP_006736                                                              16    5631
XP_005263233                                                           16    5631
NP_036320                                                              15    9605
NP_001184033                                                           15    9605
NP_005924                                                              15    9605
NP_001139114                                                           15    9605
NP_055502                                                              15    9605
NP_112558                                                              15    9605
NP_002604                                                              15    9605
NP_001248745                                                           15    9605
XP_005255413                                                           15    9605
XP_006713894                                                           15    9605
XP_006713893                                                           15    9605
XP_006713892                                                           15    9605
XP_005269431                                                           15    9605
XP_006713891                                                           15    9605
XP_006713890                                                           15    9605
XP_005275825                                                           15    9605
XP_006710061                                                           15    9605
XP_003960932                                                           15    9605
XP_006710060                                                           15    9605
>NP_001257310
          Length = 517

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSTASLAKE 9
           FST+ +AKE
Sbjct: 103 FSTSEIAKE 111
>XP_005273823
          Length = 758

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FSTASLAKE 9
           FST+ +AKE
Sbjct: 344 FSTSEIAKE 352
>NP_006736
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  FSTASLAKE 9
          FS+ASLA E
Sbjct: 10 FSSASLAVE 18
>XP_005263233
          Length = 293

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1  FSTASLAKE 9
          FS+ASLA E
Sbjct: 10 FSSASLAVE 18
>NP_036320
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   STASLAKE 9
           STASLA E
Sbjct: 225 STASLALE 232
>NP_001184033
          Length = 3972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSTASLAKE 9
           FS+A  AKE
Sbjct: 632 FSSAKYAKE 640
>NP_005924
          Length = 3969

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSTASLAKE 9
           FS+A  AKE
Sbjct: 632 FSSAKYAKE 640
>NP_001139114
          Length = 927

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 778 FKTCRLAKE 786
>NP_055502
          Length = 972

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 823 FKTCRLAKE 831
>NP_112558
          Length = 429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSTASLAKE 9
           FST  LA+E
Sbjct: 93  FSTVVLARE 101
>NP_002604
          Length = 556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSTASLAKE 9
           FST  LA+E
Sbjct: 93  FSTVVLARE 101
>NP_001248745
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FSTASLAKE 9
           FST  LA+E
Sbjct: 93  FSTVVLARE 101
>XP_005255413
          Length = 529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FSTASLAKE 9
          FST  LA+E
Sbjct: 66 FSTVVLARE 74
>XP_006713894
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 696 FKTCRLAKE 704
>XP_006713893
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 696 FKTCRLAKE 704
>XP_006713892
          Length = 952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 803 FKTCRLAKE 811
>XP_005269431
          Length = 987

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 838 FKTCRLAKE 846
>XP_006713891
          Length = 997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 848 FKTCRLAKE 856
>XP_006713890
          Length = 1012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   FSTASLAKE 9
           F T  LAKE
Sbjct: 863 FKTCRLAKE 871
>XP_005275825
          Length = 218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FSTASLAKE 9
          FST  LA+E
Sbjct: 66 FSTVVLARE 74
>XP_006710061
          Length = 298

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FSTASLAKE 9
          FST  LA+E
Sbjct: 66 FSTVVLARE 74
>XP_003960932
          Length = 396

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FSTASLAKE 9
          FST  LA+E
Sbjct: 66 FSTVVLARE 74
>XP_006710060
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  FSTASLAKE 9
          FST  LA+E
Sbjct: 66 FSTVVLARE 74
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.108    0.248 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 242,242
Number of extensions: 323
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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