Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           20     509
NP_000240                                                              20     509
XP_005265218                                                           20     509
NP_055196                                                              18    1135
NP_001035197                                                           18    1135
XP_005267591                                                           18    1135
XP_005267590                                                           18    1135
XP_005267589                                                           18    1135
XP_005267588                                                           18    1135
XP_006720179                                                           18    1135
NP_078966                                                              17    3301
NP_000258                                                              17    4312
NP_001035957                                                           17    4312
NP_031394                                                              17    4312
XP_006721991                                                           17    4312
XP_006721990                                                           17    4312
XP_006721989                                                           17    4312
XP_005258041                                                           17    4312
XP_006721988                                                           17    4312
XP_006721987                                                           17    4312
XP_006721986                                                           17    4312
XP_005258040                                                           17    4312
XP_006721985                                                           17    4312
NP_004949                                                              16    7355
NP_001264044                                                           16    7355
NP_065875                                                              16    7355
NP_006169                                                              16    7355
XP_006725689                                                           16    7355
XP_006725339                                                           16    7355
XP_006721992                                                           16    7355
XP_005252604                                                           16    7355
XP_005252603                                                           16    7355
XP_005252601                                                           16    7355
XP_006717551                                                           16    7355
XP_005252600                                                           16    7355
XP_005252599                                                           16    7355
XP_006717550                                                           16    7355
XP_005263496                                                           16    7355
XP_005263495                                                           16    7355
NP_068798                                                              15    9605
NP_003927                                                              15    9605
NP_060198                                                              15    9605
XP_006722026                                                           15    9605
>NP_001245200
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALASI
Sbjct: 99  FRGEALASI 107
>NP_000240
          Length = 756

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALASI
Sbjct: 99  FRGEALASI 107
>XP_005265218
          Length = 687

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALASI
Sbjct: 99  FRGEALASI 107
>NP_055196
          Length = 1429

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>NP_001035197
          Length = 1453

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>XP_005267591
          Length = 1275

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>XP_005267590
          Length = 1394

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>XP_005267589
          Length = 1418

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>XP_005267588
          Length = 1453

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>XP_006720179
          Length = 1453

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   FRGDALASI 9
           FRG+ALA+I
Sbjct: 92  FRGEALANI 100
>NP_078966
          Length = 14507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    GDALASI 9
            GDALASI
Sbjct: 4575 GDALASI 4581
>NP_000258
          Length = 2818

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1275 FRGNSLAS 1282
>NP_001035957
          Length = 2839

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1275 FRGNSLAS 1282
>NP_031394
          Length = 834

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   FRGDALAS 8
           FRG++LAS
Sbjct: 370 FRGNSLAS 377
>XP_006721991
          Length = 1632

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>XP_006721990
          Length = 2823

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>XP_006721989
          Length = 2823

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>XP_005258041
          Length = 2836

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1275 FRGNSLAS 1282
>XP_006721988
          Length = 2846

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>XP_006721987
          Length = 2849

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>XP_006721986
          Length = 2854

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1272 FRGNSLAS 1279
>XP_005258040
          Length = 2857

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1275 FRGNSLAS 1282
>XP_006721985
          Length = 2867

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1    FRGDALAS 8
            FRG++LAS
Sbjct: 1285 FRGNSLAS 1292
>NP_004949
          Length = 2549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    RGDALAS 8
            +GDALAS
Sbjct: 1241 QGDALAS 1247
>NP_001264044
          Length = 4516

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    FRGDALASI 9
            F GD LAS+
Sbjct: 2519 FVGDTLASL 2527
>NP_065875
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>NP_006169
          Length = 744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGDALASI 9
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_006725689
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGDALASI 9
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_006725339
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGDALASI 9
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_006721992
          Length = 769

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RGDALASI 9
           RGD LAS+
Sbjct: 477 RGDFLASL 484
>XP_005252604
          Length = 1386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252603
          Length = 1457

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252601
          Length = 1948

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 788 GDSLASI 794
>XP_006717551
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252600
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005252599
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_006717550
          Length = 1958

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   GDALASI 9
           GD+LASI
Sbjct: 798 GDSLASI 804
>XP_005263496
          Length = 2322

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    RGDALAS 8
            +GDALAS
Sbjct: 1014 QGDALAS 1020
>XP_005263495
          Length = 2549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2    RGDALAS 8
            +GDALAS
Sbjct: 1241 QGDALAS 1247
>NP_068798
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   FRGDALAS 8
           F GDAL S
Sbjct: 408 FSGDALES 415
>NP_003927
          Length = 367

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   RGDALAS 8
           RGD LAS
Sbjct: 248 RGDELAS 254
>NP_060198
          Length = 496

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGDAL 6
           FRGD+L
Sbjct: 329 FRGDSL 334
>XP_006722026
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   FRGDAL 6
           FRGD+L
Sbjct: 328 FRGDSL 333
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.144    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 492,274
Number of extensions: 563
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package