Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              19     665
XP_005251512                                                           19     665
NP_057431                                                              17    4312
NP_065908                                                              16    5631
NP_060502                                                              16    7355
NP_958815                                                              16    7355
NP_689596                                                              16    7355
NP_001231514                                                           16    7355
NP_079019                                                              16    7355
NP_001231513                                                           16    7355
NP_001193856                                                           16    7355
NP_001257572                                                           16    7355
NP_057103                                                              16    7355
XP_006720967                                                           16    7355
XP_005255484                                                           16    7355
XP_006720966                                                           16    7355
XP_005255483                                                           16    7355
XP_006715067                                                           16    7355
NP_002326                                                              15    9605
NP_001017975                                                           15    9605
NP_057346                                                              15    9605
NP_008849                                                              15    9605
XP_005274051                                                           15    9605
XP_006710458                                                           15    9605
NP_001020127                                                           15    9605
>NP_004963
          Length = 1132

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FMDLALRVD 9
            F DLALRVD
Sbjct: 1116 FRDLALRVD 1124
>XP_005251512
          Length = 1132

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    FMDLALRVD 9
            F DLALRVD
Sbjct: 1116 FRDLALRVD 1124
>NP_057431
          Length = 227

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  FMDLALRVD 9
          ++D+ALR D
Sbjct: 85 YVDIALRTD 93
>NP_065908
          Length = 973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MDLALRVD 9
           +DLAL+VD
Sbjct: 727 VDLALQVD 734
>NP_060502
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 64 DLALRAD 70
>NP_958815
          Length = 371

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 64 DLALRAD 70
>NP_689596
          Length = 885

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   FMDLALRVD 9
           F+D ALR+D
Sbjct: 544 FVDGALRLD 552
>NP_001231514
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 61 DLALRAD 67
>NP_079019
          Length = 527

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  FMDLALRV 8
          F+DLAL V
Sbjct: 90 FVDLALNV 97
>NP_001231513
          Length = 458

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   DLALRVD 9
           DLALR D
Sbjct: 130 DLALRAD 136
>NP_001193856
          Length = 4707

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FMDLALRVD 9
            F+DL  R+D
Sbjct: 1549 FLDLKKRID 1557
>NP_001257572
          Length = 391

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 63 DLALRAD 69
>NP_057103
          Length = 392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 64 DLALRAD 70
>XP_006720967
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 11 DLALRAD 17
>XP_005255484
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 11 DLALRAD 17
>XP_006720966
          Length = 318

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 11 DLALRAD 17
>XP_005255483
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  DLALRVD 9
          DLALR D
Sbjct: 64 DLALRAD 70
>XP_006715067
          Length = 4707

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FMDLALRVD 9
            F+DL  R+D
Sbjct: 1549 FLDLKKRID 1557
>NP_002326
          Length = 1615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMDLALRVD 9
           F D+ L+VD
Sbjct: 362 FTDIVLQVD 370
>NP_001017975
          Length = 1435

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FMDLALRVD 9
           F+D+ LR++
Sbjct: 819 FLDIQLRIN 827
>NP_057346
          Length = 475

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  FMDLALR 7
          F DLALR
Sbjct: 20 FWDLALR 26
>NP_008849
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2 MDLALRV 8
         MDL LRV
Sbjct: 1 MDLVLRV 7
>XP_005274051
          Length = 1653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FMDLALRVD 9
           F D+ L+VD
Sbjct: 362 FTDIVLQVD 370
>XP_006710458
          Length = 846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   FMDLALRVD 9
           F+D+ LR++
Sbjct: 230 FLDIQLRIN 238
>NP_001020127
          Length = 299

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2 MDLALRV 8
         MDL LRV
Sbjct: 1 MDLVLRV 7
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.155    0.439 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 356,364
Number of extensions: 539
Number of successful extensions: 25
Number of sequences better than 10000.0: 25
Number of HSP's gapped: 25
Number of HSP's successfully gapped: 25
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)

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