Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057666                                                              17    2528
NP_060016                                                              17    3301
XP_006723595                                                           17    3301
XP_005277007                                                           16    5631
XP_005277005                                                           16    5631
XP_005258778                                                           16    5631
XP_005258776                                                           16    5631
XP_005268213                                                           16    5631
NP_001107565                                                           16    5631
NP_056393                                                              16    5631
NP_065750                                                              16    7355
NP_060130                                                              16    7355
NP_055858                                                              16    7355
NP_942568                                                              16    7355
XP_005260019                                                           16    7355
XP_005260018                                                           16    7355
XP_005260017                                                           16    7355
XP_005260016                                                           16    7355
XP_005260015                                                           16    7355
XP_006722844                                                           16    7355
XP_005260014                                                           16    7355
XP_006722843                                                           16    7355
XP_005260013                                                           16    7355
XP_005260011                                                           16    7355
XP_005269123                                                           16    7355
XP_005269118                                                           16    7355
XP_006719607                                                           16    7355
XP_005269117                                                           16    7355
XP_006719606                                                           16    7355
XP_006719605                                                           16    7355
XP_005269116                                                           16    7355
XP_006719604                                                           16    7355
XP_005245421                                                           16    7355
XP_006711532                                                           16    7355
XP_005245420                                                           16    7355
NP_002889                                                              16    7355
NP_056030                                                              15    9605
NP_001138760                                                           15    9605
NP_647480                                                              15    9605
NP_055523                                                              15    9605
NP_775853                                                              15    9605
NP_694539                                                              15    9605
NP_612152                                                              15    9605
NP_001180404                                                           15    9605
NP_001803                                                              15    9605
NP_001193599                                                           15    9605
NP_001129101                                                           15    9605
XP_005267442                                                           15    9605
XP_006719769                                                           15    9605
XP_006715715                                                           15    9605
XP_006714127                                                           15    9605
XP_006710546                                                           15    9605
XP_006710545                                                           15    9605
NP_000963                                                              15    9605
NP_001028260                                                           15    9605
>NP_057666
          Length = 581

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   LPSAAQKM 9
           LPSA QKM
Sbjct: 404 LPSALQKM 411
>NP_060016
          Length = 510

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   LPSAAQKM 9
           LPSAAQ++
Sbjct: 118 LPSAAQRL 125
>XP_006723595
          Length = 678

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   FLPSAAQK 8
           F+PS AQK
Sbjct: 195 FIPSQAQK 202
>XP_005277007
          Length = 426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>XP_005277005
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>XP_005258778
          Length = 426

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>XP_005258776
          Length = 487

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>XP_005268213
          Length = 993

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   FLPSAAQKM 9
           FLP+ A+K+
Sbjct: 270 FLPAVAEKV 278
>NP_001107565
          Length = 463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>NP_056393
          Length = 463

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           LPSA+QK
Sbjct: 223 LPSASQK 229
>NP_065750
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 379 FLPSAA 384
>NP_060130
          Length = 633

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 375 FLPSAA 380
>NP_055858
          Length = 1233

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLPSAAQKM 9
           FLP  ++KM
Sbjct: 649 FLPQLSEKM 657
>NP_942568
          Length = 1250

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLPSAAQKM 9
           FLP  ++KM
Sbjct: 649 FLPQLSEKM 657
>XP_005260019
          Length = 543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 284 FLPSAA 289
>XP_005260018
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 375 FLPSAA 380
>XP_005260017
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 375 FLPSAA 380
>XP_005260016
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 375 FLPSAA 380
>XP_005260015
          Length = 634

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 375 FLPSAA 380
>XP_006722844
          Length = 640

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 406 FLPSAA 411
>XP_005260014
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 394 FLPSAA 399
>XP_006722843
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 394 FLPSAA 399
>XP_005260013
          Length = 653

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 394 FLPSAA 399
>XP_005260011
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 406 FLPSAA 411
>XP_005269123
          Length = 214

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_005269118
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_006719607
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_005269117
          Length = 419

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_006719606
          Length = 429

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_006719605
          Length = 448

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_005269116
          Length = 448

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_006719604
          Length = 477

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>XP_005245421
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 379 FLPSAA 384
>XP_006711532
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 379 FLPSAA 384
>XP_005245420
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   FLPSAA 6
           FLPSAA
Sbjct: 385 FLPSAA 390
>NP_002889
          Length = 407

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   PSAAQK 8
           PSAAQK
Sbjct: 107 PSAAQK 112
>NP_056030
          Length = 1673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LPSAAQ 7
            LPSAAQ
Sbjct: 1450 LPSAAQ 1455
>NP_001138760
          Length = 539

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FLPSAAQ 7
           F+PSAA+
Sbjct: 173 FIPSAAR 179
>NP_647480
          Length = 589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FLPSAAQ 7
           F+PSAA+
Sbjct: 173 FIPSAAR 179
>NP_055523
          Length = 2209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LPSAAQ 7
            LPSAAQ
Sbjct: 1583 LPSAAQ 1588
>NP_775853
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLPSAAQKM 9
           F+P A QK+
Sbjct: 156 FVPYAVQKL 164
>NP_694539
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LPSAAQKM 9
           LP+A QK+
Sbjct: 455 LPTATQKL 462
>NP_612152
          Length = 2849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    FLPSAAQK 8
            ++P+A+QK
Sbjct: 1646 YIPAASQK 1653
>NP_001180404
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LPSAAQKM 9
           LP+A QK+
Sbjct: 455 LPTATQKL 462
>NP_001803
          Length = 943

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           +PS+AQK
Sbjct: 174 IPSSAQK 180
>NP_001193599
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   FLPSAAQK 8
           +LP+A+QK
Sbjct: 96  WLPTASQK 103
>NP_001129101
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   LPSAAQKM 9
           LP+A QK+
Sbjct: 455 LPTATQKL 462
>XP_005267442
          Length = 650

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   LPSAAQK 8
           LPS AQK
Sbjct: 244 LPSEAQK 250
>XP_006719769
          Length = 2209

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    LPSAAQ 7
            LPSAAQ
Sbjct: 1583 LPSAAQ 1588
>XP_006715715
          Length = 2023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1    FLPSAAQK 8
            ++P+A+QK
Sbjct: 1598 YIPAASQK 1605
>XP_006714127
          Length = 935

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LPSAAQK 8
           +PS+AQK
Sbjct: 174 IPSSAQK 180
>XP_006710546
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LPSAAQ 7
           LPSAAQ
Sbjct: 474 LPSAAQ 479
>XP_006710545
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   LPSAAQ 7
           LPSAAQ
Sbjct: 474 LPSAAQ 479
>NP_000963
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLPSAAQKM 9
           FLP+  +KM
Sbjct: 169 FLPALCRKM 177
>NP_001028260
          Length = 291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FLPSAAQKM 9
           FLP+  QK+
Sbjct: 126 FLPTLEQKL 134
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.324    0.125    0.344 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 673,881
Number of extensions: 924
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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