Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              20     509
NP_671714                                                              20     509
XP_006715890                                                           20     509
XP_005250160                                                           20     509
XP_005250159                                                           20     509
NP_001242904                                                           17    4312
NP_001008496                                                           17    4312
NP_001360                                                              16    5631
XP_005248319                                                           16    5631
NP_001138766                                                           16    7355
NP_055786                                                              16    7355
NP_060767                                                              16    7355
NP_001138765                                                           16    7355
NP_055952                                                              16    7355
XP_005250906                                                           16    7355
XP_005265373                                                           16    7355
NP_004374                                                              15    9605
NP_001120662                                                           15    9605
NP_055950                                                              15    9605
NP_001001998                                                           15    9605
NP_062535                                                              15    9605
XP_005254222                                                           15    9605
XP_005267917                                                           15    9605
XP_006716564                                                           15    9605
XP_005250826                                                           15    9605
XP_005250292                                                           15    9605
XP_005263533                                                           15    9605
XP_005263532                                                           15    9605
NP_001106                                                              15    9605
NP_002676                                                              15    9605
>NP_005742
          Length = 3907

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FIVRQSIND 9
            FIVRQSI+D
Sbjct: 1559 FIVRQSIHD 1567
>NP_671714
          Length = 3899

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FIVRQSIND 9
            FIVRQSI+D
Sbjct: 1559 FIVRQSIHD 1567
>XP_006715890
          Length = 3860

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FIVRQSIND 9
            FIVRQSI+D
Sbjct: 1559 FIVRQSIHD 1567
>XP_005250160
          Length = 3939

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FIVRQSIND 9
            FIVRQSI+D
Sbjct: 1559 FIVRQSIHD 1567
>XP_005250159
          Length = 3952

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    FIVRQSIND 9
            FIVRQSI+D
Sbjct: 1559 FIVRQSIHD 1567
>NP_001242904
          Length = 873

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FIVRQSIND 9
           FIV QS+ D
Sbjct: 790 FIVSQSVQD 798
>NP_001008496
          Length = 882

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FIVRQSIND 9
           FIV QS+ D
Sbjct: 799 FIVSQSVQD 807
>NP_001360
          Length = 4624

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FIVRQSIN 8
            F++ QSIN
Sbjct: 2453 FVITQSIN 2460
>XP_005248319
          Length = 4609

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    FIVRQSIN 8
            F++ QSIN
Sbjct: 2438 FVITQSIN 2445
>NP_001138766
          Length = 612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           + RQS+ND
Sbjct: 590 LFRQSLND 597
>NP_055786
          Length = 817

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVRQSIND 9
           +VR+++ND
Sbjct: 174 VVRKTVND 181
>NP_060767
          Length = 654

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           + RQS+ND
Sbjct: 632 LFRQSLND 639
>NP_001138765
          Length = 636

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           + RQS+ND
Sbjct: 614 LFRQSLND 621
>NP_055952
          Length = 821

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVRQSIND 9
           +VR+++ND
Sbjct: 175 VVRKTVND 182
>XP_005250906
          Length = 785

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   IVRQSIND 9
           +VR+++ND
Sbjct: 139 VVRKTVND 146
>XP_005265373
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           + RQS+ND
Sbjct: 577 LFRQSLND 584
>NP_004374
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FIVRQSIN 8
           F+VR+S N
Sbjct: 104 FLVRESTN 111
>NP_001120662
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FIVRQSIN 8
           F+VR+S N
Sbjct: 104 FLVRESTN 111
>NP_055950
          Length = 2012

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    FIVRQSIND 9
            ++++QS ND
Sbjct: 1817 YLLKQSAND 1825
>NP_001001998
          Length = 885

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           +VRQ IN+
Sbjct: 561 LVRQQINE 568
>NP_062535
          Length = 2146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FIVRQSIND 9
            FI+  +IND
Sbjct: 1928 FIILDAIND 1936
>XP_005254222
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   FIVRQSIN 8
           F+VR+S N
Sbjct: 104 FLVRESTN 111
>XP_005267917
          Length = 2061

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    FIVRQSIND 9
            FI+  +IND
Sbjct: 1928 FIILDAIND 1936
>XP_006716564
          Length = 1185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FIVRQSIND 9
           F+V + IND
Sbjct: 381 FVVLEMIND 389
>XP_005250826
          Length = 1221

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FIVRQSIND 9
           F+V + IND
Sbjct: 381 FVVLEMIND 389
>XP_005250292
          Length = 1654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    FIVRQSIND 9
            ++++QS ND
Sbjct: 1459 YLLKQSAND 1467
>XP_005263533
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           +VRQ IN+
Sbjct: 129 LVRQQINE 136
>XP_005263532
          Length = 914

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           +VRQ IN+
Sbjct: 585 LVRQQINE 592
>NP_001106
          Length = 1251

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FIVRQSIND 9
           F+V + IND
Sbjct: 381 FVVLEMIND 389
>NP_002676
          Length = 860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IVRQSIND 9
           +VRQ IN+
Sbjct: 561 LVRQQINE 568
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.333    0.146    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 314,305
Number of extensions: 509
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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