Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     299
NP_004739                                                              18    1935
NP_001191379                                                           18    1935
NP_065790                                                              18    1935
NP_001540                                                              17    2528
NP_001276688                                                           17    2528
NP_001276687                                                           17    2528
NP_001026853                                                           17    2528
NP_001268695                                                           17    3301
NP_872272                                                              17    3301
NP_001191380                                                           17    3301
NP_060787                                                              17    3301
NP_004430                                                              17    3301
NP_001268694                                                           17    3301
NP_001268696                                                           17    3301
XP_005265710                                                           17    3301
XP_005248229                                                           17    3301
XP_006714030                                                           17    3301
NP_001538                                                              17    3301
NP_000928                                                              16    5631
NP_060650                                                              16    5631
NP_065822                                                              16    7355
NP_001127854                                                           16    7355
NP_055684                                                              16    7355
NP_005179                                                              16    7355
NP_001137485                                                           16    7355
XP_005273659                                                           16    7355
XP_006716448                                                           16    7355
XP_006716447                                                           16    7355
XP_006716446                                                           16    7355
XP_006716445                                                           16    7355
XP_006716444                                                           16    7355
XP_005267130                                                           16    7355
XP_006715593                                                           16    7355
XP_005267128                                                           16    7355
XP_006715592                                                           16    7355
XP_006715591                                                           16    7355
XP_006712771                                                           16    7355
NP_073586                                                              16    7355
NP_001273886                                                           15    9605
NP_149115                                                              15    9605
NP_002030                                                              15    9605
NP_997006                                                              15    9605
NP_002058                                                              15    9605
NP_055279                                                              15    9605
NP_071940                                                              15    9605
NP_001240795                                                           15    9605
NP_940999                                                              15    9605
XP_005268066                                                           15    9605
XP_006715127                                                           15    9605
XP_006715125                                                           15    9605
XP_006715124                                                           15    9605
XP_005262939                                                           15    9605
XP_005262938                                                           15    9605
NP_004913                                                              15    9605
>NP_443179
          Length = 2170

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  FENQYIRVL 9
          FENQYI VL
Sbjct: 64 FENQYIHVL 72
>NP_004739
          Length = 757

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQY+RV
Sbjct: 306 ENQYLRV 312
>NP_001191379
          Length = 807

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQY+RV
Sbjct: 306 ENQYLRV 312
>NP_065790
          Length = 757

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQY+RV
Sbjct: 306 ENQYLRV 312
>NP_001540
          Length = 490

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           +NQY++VL
Sbjct: 205 DNQYVKVL 212
>NP_001276688
          Length = 438

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           +NQY++VL
Sbjct: 153 DNQYVKVL 160
>NP_001276687
          Length = 438

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           +NQY++VL
Sbjct: 153 DNQYVKVL 160
>NP_001026853
          Length = 490

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           +NQY++VL
Sbjct: 205 DNQYVKVL 212
>NP_001268695
          Length = 1016

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>NP_872272
          Length = 1015

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>NP_001191380
          Length = 424

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ENQYIRVL 9
           ENQY+R L
Sbjct: 306 ENQYLRKL 313
>NP_060787
          Length = 526

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FENQYIRV 8
           FEN+++R+
Sbjct: 224 FENEHVRI 231
>NP_004430
          Length = 1037

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>NP_001268694
          Length = 1038

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>NP_001268696
          Length = 1004

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>XP_005265710
          Length = 874

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   ENQYIRV 8
           ENQYI++
Sbjct: 168 ENQYIKI 174
>XP_005248229
          Length = 461

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FENQYIRV 8
           FEN+++R+
Sbjct: 159 FENEHVRI 166
>XP_006714030
          Length = 495

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   FENQYIRV 8
           FEN+++R+
Sbjct: 193 FENEHVRI 200
>NP_001538
          Length = 472

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           +NQY++VL
Sbjct: 207 DNQYLKVL 214
>NP_000928
          Length = 1970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FENQ  R+L
Sbjct: 737 FENQVNRIL 745
>NP_060650
          Length = 743

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ENQYIRV 8
           EN YIR+
Sbjct: 674 ENNYIRI 680
>NP_065822
          Length = 909

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 285 ESQYLKIL 292
>NP_001127854
          Length = 1114

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   FENQY 5
           FENQY
Sbjct: 802 FENQY 806
>NP_055684
          Length = 963

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 1   FENQY 5
           FENQY
Sbjct: 816 FENQY 820
>NP_005179
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   ENQYIRV 8
           EN+Y RV
Sbjct: 111 ENEYFRV 117
>NP_001137485
          Length = 1758

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1    FENQYIRVL 9
            ++N+++R+L
Sbjct: 1388 YDNKHVRIL 1396
>XP_005273659
          Length = 629

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 599 FEGHFLRVL 607
>XP_006716448
          Length = 650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 496 FEGHFLRVL 504
>XP_006716447
          Length = 650

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 496 FEGHFLRVL 504
>XP_006716446
          Length = 695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 541 FEGHFLRVL 549
>XP_006716445
          Length = 695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 541 FEGHFLRVL 549
>XP_006716444
          Length = 753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 599 FEGHFLRVL 607
>XP_005267130
          Length = 573

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 285 ESQYLKIL 292
>XP_006715593
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 117 ESQYLKIL 124
>XP_005267128
          Length = 741

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 117 ESQYLKIL 124
>XP_006715592
          Length = 865

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 241 ESQYLKIL 248
>XP_006715591
          Length = 875

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   ENQYIRVL 9
           E+QY+++L
Sbjct: 251 ESQYLKIL 258
>XP_006712771
          Length = 1766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/9 (33%), Positives = 9/9 (100%)

Query: 1    FENQYIRVL 9
            ++N+++R+L
Sbjct: 1388 YDNKHVRIL 1396
>NP_073586
          Length = 743

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE  ++RVL
Sbjct: 599 FEGHFLRVL 607
>NP_001273886
          Length = 341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ENQYIR 7
           EN+YIR
Sbjct: 146 ENRYIR 151
>NP_149115
          Length = 484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   ENQYIR 7
           EN+YIR
Sbjct: 289 ENRYIR 294
>NP_002030
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  FENQYI 6
          FEN YI
Sbjct: 79 FENSYI 84
>NP_997006
          Length = 724

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  FENQYI 6
          FEN YI
Sbjct: 79 FENSYI 84
>NP_002058
          Length = 359

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   FENQYIRVL 9
           FE+QY+  +
Sbjct: 124 FEHQYVSAI 132
>NP_055279
          Length = 476

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ENQYIRVL 9
           EN Y R+L
Sbjct: 444 ENGYFRIL 451
>NP_071940
          Length = 938

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FENQYIRV 8
           FE +Y RV
Sbjct: 473 FEQEYTRV 480
>NP_001240795
          Length = 683

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   FENQYI 6
           FEN+Y+
Sbjct: 106 FENKYV 111
>NP_940999
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FENQYIRV 8
           F NQY+ V
Sbjct: 699 FPNQYVDV 706
>XP_005268066
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FENQYIRV 8
           FE +Y RV
Sbjct: 707 FEQEYTRV 714
>XP_006715127
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ENQYIRVL 9
           EN Y R+L
Sbjct: 263 ENGYFRIL 270
>XP_006715125
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ENQYIRVL 9
           EN Y R+L
Sbjct: 426 ENGYFRIL 433
>XP_006715124
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   ENQYIRVL 9
           EN Y R+L
Sbjct: 447 ENGYFRIL 454
>XP_005262939
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  FENQYI 6
          FEN YI
Sbjct: 79 FENSYI 84
>XP_005262938
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1  FENQYI 6
          FEN YI
Sbjct: 79 FENSYI 84
>NP_004913
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   FENQYIRV 8
           F NQY+ V
Sbjct: 699 FPNQYVDV 706
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.152    0.430 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 548,187
Number of extensions: 1079
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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