Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_057502                                                              17    3301
NP_861969                                                              17    3301
NP_000259                                                              17    3301
NP_861968                                                              17    3301
NP_861966                                                              17    3301
NP_861970                                                              17    3301
NP_861546                                                              17    3301
NP_861967                                                              17    3301
NP_001135973                                                           16    7355
NP_115731                                                              16    7355
NP_071735                                                              16    7355
NP_001230131                                                           16    7355
NP_036548                                                              16    7355
XP_005265443                                                           16    7355
XP_005265442                                                           16    7355
XP_005265441                                                           16    7355
XP_005265440                                                           16    7355
NP_002821                                                              15    9605
NP_005898                                                              15    9605
XP_005267043                                                           15    9605
>NP_057502
          Length = 590

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 222 FAIRNKK 228
>NP_861969
          Length = 507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 139 FAIRNKK 145
>NP_000259
          Length = 595

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 222 FAIRNKK 228
>NP_861968
          Length = 507

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 139 FAIRNKK 145
>NP_861966
          Length = 548

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 180 FAIRNKK 186
>NP_861970
          Length = 590

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 222 FAIRNKK 228
>NP_861546
          Length = 590

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 222 FAIRNKK 228
>NP_861967
          Length = 549

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           FAIRNK+
Sbjct: 181 FAIRNKK 187
>NP_001135973
          Length = 490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IRNKRAQ 9
          +RN+RAQ
Sbjct: 90 LRNRRAQ 96
>NP_115731
          Length = 652

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  IRNKRAQ 9
          +RN+RAQ
Sbjct: 90 LRNRRAQ 96
>NP_071735
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           F+IRN+R
Sbjct: 170 FSIRNRR 176
>NP_001230131
          Length = 753

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AIRNKRA 8
          A+RN+RA
Sbjct: 56 AVRNRRA 62
>NP_036548
          Length = 1105

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  AIRNKRA 8
          A+RN+RA
Sbjct: 56 AVRNRRA 62
>XP_005265443
          Length = 695

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  FAIRNKR 7
          F+IRN+R
Sbjct: 81 FSIRNRR 87
>XP_005265442
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           F+IRN+R
Sbjct: 170 FSIRNRR 176
>XP_005265441
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           F+IRN+R
Sbjct: 170 FSIRNRR 176
>XP_005265440
          Length = 784

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   FAIRNKR 7
           F+IRN+R
Sbjct: 170 FSIRNRR 176
>NP_002821
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   IRNKRA 8
           +RNKRA
Sbjct: 837 VRNKRA 842
>NP_005898
          Length = 653

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIRNKRAQ 9
           AIR KRA+
Sbjct: 191 AIREKRAK 198
>XP_005267043
          Length = 736

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   AIRNKRAQ 9
           AIR KRA+
Sbjct: 274 AIREKRAK 281
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.135    0.364 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 359,350
Number of extensions: 307
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)

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