Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           18    1135
NP_001020014                                                           18    1135
NP_055903                                                              18    1135
NP_001275648                                                           17    3301
NP_001275647                                                           17    3301
NP_001275649                                                           17    3301
NP_003143                                                              17    3301
NP_001191111                                                           17    3301
XP_005257681                                                           17    3301
NP_002438                                                              17    4312
NP_001231866                                                           17    4312
XP_005265227                                                           17    4312
NP_277045                                                              16    5631
NP_001137440                                                           16    5631
NP_114431                                                              16    7355
NP_001156889                                                           16    7355
NP_001156890                                                           16    7355
NP_001167554                                                           16    7355
NP_001167553                                                           16    7355
NP_060322                                                              16    7355
NP_001167552                                                           16    7355
XP_005272167                                                           16    7355
XP_005272166                                                           16    7355
XP_006714782                                                           16    7355
XP_006714781                                                           16    7355
XP_005272165                                                           16    7355
XP_005272164                                                           16    7355
XP_005272163                                                           16    7355
XP_005272162                                                           16    7355
XP_005272161                                                           16    7355
XP_005272160                                                           16    7355
XP_006714780                                                           16    7355
NP_000042                                                              15    9605
NP_055737                                                              15    9605
NP_001028225                                                           15    9605
NP_127497                                                              15    9605
NP_127500                                                              15    9605
NP_127499                                                              15    9605
NP_775867                                                              15    9605
NP_001128667                                                           15    9605
NP_777603                                                              15    9605
NP_001167449                                                           15    9605
XP_005268860                                                           15    9605
XP_006718908                                                           15    9605
XP_005271621                                                           15    9605
XP_006718907                                                           15    9605
XP_006718906                                                           15    9605
XP_005271619                                                           15    9605
XP_005271618                                                           15    9605
>NP_001155973
          Length = 1833

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EVSHFLAQA 9
            EVS FLAQA
Sbjct: 1722 EVSRFLAQA 1730
>NP_001020014
          Length = 1029

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EVSHFLAQA 9
           EVS FLAQA
Sbjct: 918 EVSRFLAQA 926
>NP_055903
          Length = 1723

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EVSHFLAQA 9
            EVS FLAQA
Sbjct: 1612 EVSRFLAQA 1620
>NP_001275648
          Length = 764

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          EV H+LAQ
Sbjct: 29 EVRHYLAQ 36
>NP_001275647
          Length = 794

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          EV H+LAQ
Sbjct: 29 EVRHYLAQ 36
>NP_001275649
          Length = 763

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          EV H+LAQ
Sbjct: 29 EVRHYLAQ 36
>NP_003143
          Length = 794

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          EV H+LAQ
Sbjct: 29 EVRHYLAQ 36
>NP_001191111
          Length = 388

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SHFLAQA 9
           SHF+AQA
Sbjct: 372 SHFVAQA 378
>XP_005257681
          Length = 761

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          EV H+LAQ
Sbjct: 29 EVRHYLAQ 36
>NP_002438
          Length = 1400

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EVSHFLAQ 8
            +++HFLAQ
Sbjct: 1295 DLTHFLAQ 1302
>NP_001231866
          Length = 1351

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EVSHFLAQ 8
            +++HFLAQ
Sbjct: 1246 DLTHFLAQ 1253
>XP_005265227
          Length = 1401

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    EVSHFLAQ 8
            +++HFLAQ
Sbjct: 1296 DLTHFLAQ 1303
>NP_277045
          Length = 1401

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EVSHFLA 7
           EVSH+LA
Sbjct: 926 EVSHWLA 932
>NP_001137440
          Length = 179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EVSHFL 6
           E+SHFL
Sbjct: 137 EISHFL 142
>NP_114431
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 277 HFIAQA 282
>NP_001156889
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 231 HFIAQA 236
>NP_001156890
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 167 HFIAQA 172
>NP_001167554
          Length = 1039

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSHFLAQA 9
           ++ H LAQA
Sbjct: 249 DIPHILAQA 257
>NP_001167553
          Length = 1040

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSHFLAQA 9
           ++ H LAQA
Sbjct: 250 DIPHILAQA 258
>NP_060322
          Length = 1062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSHFLAQA 9
           ++ H LAQA
Sbjct: 272 DIPHILAQA 280
>NP_001167552
          Length = 1062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   EVSHFLAQA 9
           ++ H LAQA
Sbjct: 272 DIPHILAQA 280
>XP_005272167
          Length = 297

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 158 HFIAQA 163
>XP_005272166
          Length = 325

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 277 HFIAQA 282
>XP_006714782
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 194 HFIAQA 199
>XP_006714781
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 194 HFIAQA 199
>XP_005272165
          Length = 333

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 194 HFIAQA 199
>XP_005272164
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 277 HFIAQA 282
>XP_005272163
          Length = 343

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 204 HFIAQA 209
>XP_005272162
          Length = 352

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 213 HFIAQA 218
>XP_005272161
          Length = 370

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 231 HFIAQA 236
>XP_005272160
          Length = 388

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 277 HFIAQA 282
>XP_006714780
          Length = 416

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           HF+AQA
Sbjct: 277 HFIAQA 282
>NP_000042
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1593 EINHFLS 1599
>NP_055737
          Length = 1429

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           H+LAQA
Sbjct: 553 HYLAQA 558
>NP_001028225
          Length = 1375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           H+LAQA
Sbjct: 553 HYLAQA 558
>NP_127497
          Length = 1473

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           H+LAQA
Sbjct: 553 HYLAQA 558
>NP_127500
          Length = 1399

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           H+LAQA
Sbjct: 553 HYLAQA 558
>NP_127499
          Length = 1443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   HFLAQA 9
           H+LAQA
Sbjct: 553 HYLAQA 558
>NP_775867
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          E +H+LAQ
Sbjct: 35 EQNHYLAQ 42
>NP_001128667
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          E +H+LAQ
Sbjct: 35 EQNHYLAQ 42
>NP_777603
          Length = 122

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  SHFLAQA 9
          SH+ AQA
Sbjct: 33 SHYFAQA 39
>NP_001167449
          Length = 92

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3 SHFLAQA 9
         SH+ AQA
Sbjct: 3 SHYFAQA 9
>XP_005268860
          Length = 540

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EVSHFLAQ 8
          E +H+LAQ
Sbjct: 35 EQNHYLAQ 42
>XP_006718908
          Length = 1708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   EVSHFLA 7
           E++HFL+
Sbjct: 245 EINHFLS 251
>XP_005271621
          Length = 2708

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1245 EINHFLS 1251
>XP_006718907
          Length = 3050

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1587 EINHFLS 1593
>XP_006718906
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1593 EINHFLS 1599
>XP_005271619
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1593 EINHFLS 1599
>XP_005271618
          Length = 3056

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1    EVSHFLA 7
            E++HFL+
Sbjct: 1593 EINHFLS 1599
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.124    0.334 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 422,453
Number of extensions: 280
Number of successful extensions: 49
Number of sequences better than 10000.0: 49
Number of HSP's gapped: 49
Number of HSP's successfully gapped: 49
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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