Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005836                                                              16    5631
NP_002027                                                              16    5631
NP_001116423                                                           16    5631
XP_005254082                                                           16    5631
XP_006719977                                                           16    5631
NP_006280                                                              16    7355
NP_003310                                                              16    7355
NP_597676                                                              16    7355
NP_001243779                                                           16    7355
NP_596869                                                              16    7355
NP_689733                                                              16    7355
NP_001106869                                                           16    7355
NP_001106868                                                           16    7355
NP_001269654                                                           16    7355
XP_006716915                                                           16    7355
XP_005251621                                                           16    7355
XP_005246888                                                           16    7355
XP_006712788                                                           16    7355
XP_006712787                                                           16    7355
XP_006712786                                                           16    7355
XP_006712387                                                           16    7355
XP_006712386                                                           16    7355
XP_006712385                                                           16    7355
XP_006712384                                                           16    7355
NP_001254479                                                           16    7355
NP_597681                                                              16    7355
NP_060250                                                              15    9605
NP_006174                                                              15    9605
NP_644814                                                              15    9605
XP_006721859                                                           15    9605
XP_006716523                                                           15    9605
XP_006716522                                                           15    9605
XP_005251324                                                           15    9605
XP_005251323                                                           15    9605
>NP_005836
          Length = 1325

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EVKALEATT 9
           +VK LE+TT
Sbjct: 888 DVKRLESTT 896
>NP_002027
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           E+KAL+AT
Sbjct: 202 ELKALQAT 209
>NP_001116423
          Length = 338

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           E+KAL+AT
Sbjct: 204 ELKALQAT 211
>XP_005254082
          Length = 1282

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EVKALEATT 9
           +VK LE+TT
Sbjct: 845 DVKRLESTT 853
>XP_006719977
          Length = 1295

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EVKALEATT 9
           +VK LE+TT
Sbjct: 858 DVKRLESTT 866
>NP_006280
          Length = 2541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    KALEATT 9
            +ALEATT
Sbjct: 2144 RALEATT 2150
>NP_003310
          Length = 26926

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 18850 QVKTLEAT 18857
>NP_597676
          Length = 27051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 18975 QVKTLEAT 18982
>NP_001243779
          Length = 34350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 26274 QVKTLEAT 26281
>NP_596869
          Length = 33423

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 25347 QVKTLEAT 25354
>NP_689733
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 184 KVKALDAT 191
>NP_001106869
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  EVKALEAT 8
          +VKAL+AT
Sbjct: 82 KVKALDAT 89
>NP_001106868
          Length = 395

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 108 KVKALDAT 115
>NP_001269654
          Length = 369

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  EVKALEAT 8
          +VKAL+AT
Sbjct: 82 KVKALDAT 89
>XP_006716915
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    KALEATT 9
            +ALEATT
Sbjct: 2144 RALEATT 2150
>XP_005251621
          Length = 2542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3    KALEATT 9
            +ALEATT
Sbjct: 2144 RALEATT 2150
>XP_005246888
          Length = 22663

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 14587 QVKTLEAT 14594
>XP_006712788
          Length = 24022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 15946 QVKTLEAT 15953
>XP_006712787
          Length = 33978

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 25902 QVKTLEAT 25909
>XP_006712786
          Length = 35484

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 27408 QVKTLEAT 27415
>XP_006712387
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 95  KVKALDAT 102
>XP_006712386
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 95  KVKALDAT 102
>XP_006712385
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 95  KVKALDAT 102
>XP_006712384
          Length = 382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EVKALEAT 8
           +VKAL+AT
Sbjct: 95  KVKALDAT 102
>NP_001254479
          Length = 35991

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 27915 QVKTLEAT 27922
>NP_597681
          Length = 27118

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1     EVKALEAT 8
             +VK LEAT
Sbjct: 19042 QVKTLEAT 19049
>NP_060250
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    EVKALEA 7
            EV+ALEA
Sbjct: 1947 EVRALEA 1953
>NP_006174
          Length = 170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  EVKALEA 7
          E+KALEA
Sbjct: 29 EMKALEA 35
>NP_644814
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VKALEATT 9
           ++A+EATT
Sbjct: 440 LRAIEATT 447
>XP_006721859
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   VKALEATT 9
           ++A+EATT
Sbjct: 404 LRAIEATT 411
>XP_006716523
          Length = 1912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   EVKALEA 7
           EV+ALEA
Sbjct: 862 EVRALEA 868
>XP_006716522
          Length = 2192

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    EVKALEA 7
            EV+ALEA
Sbjct: 1142 EVRALEA 1148
>XP_005251324
          Length = 2996

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    EVKALEA 7
            EV+ALEA
Sbjct: 1947 EVRALEA 1953
>XP_005251323
          Length = 2997

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1    EVKALEA 7
            EV+ALEA
Sbjct: 1947 EVRALEA 1953
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.106    0.236 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 261,498
Number of extensions: 236
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)

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