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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1935
NP_671714                                                              18    1935
XP_006715890                                                           18    1935
XP_005250160                                                           18    1935
XP_005250159                                                           18    1935
NP_076975                                                              16    5631
NP_001243888                                                           16    5631
NP_001277                                                              16    5631
XP_006716188                                                           16    5631
NP_078966                                                              15    9605
NP_001724                                                              15    9605
XP_006723004                                                           15    9605

>NP_005742
          Length = 3907

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            +SQSSASLI
Sbjct: 1762 KSQSSASLI 1770



>NP_671714
          Length = 3899

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            +SQSSASLI
Sbjct: 1762 KSQSSASLI 1770



>XP_006715890
          Length = 3860

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            +SQSSASLI
Sbjct: 1715 KSQSSASLI 1723



>XP_005250160
          Length = 3939

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            +SQSSASLI
Sbjct: 1794 KSQSSASLI 1802



>XP_005250159
          Length = 3952

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            +SQSSASLI
Sbjct: 1794 KSQSSASLI 1802



>NP_076975
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ESQSSASLI 9
           E+QSS SLI
Sbjct: 109 ETQSSISLI 117



>NP_001243888
          Length = 847

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   ESQSSAS 7
           ESQSSAS
Sbjct: 740 ESQSSAS 746



>NP_001277
          Length = 869

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   ESQSSAS 7
           ESQSSAS
Sbjct: 762 ESQSSAS 768



>XP_006716188
          Length = 326

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ESQSSASLI 9
           E+QSS SLI
Sbjct: 109 ETQSSISLI 117



>NP_078966
          Length = 14507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1     ESQSSASLI 9
             ES+++ASL+
Sbjct: 10564 ESETTASLV 10572



>NP_001724
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   ESQSSASL 8
           E+QS+ASL
Sbjct: 510 EAQSNASL 517



>XP_006723004
          Length = 6443

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1    ESQSSASLI 9
            ES+++ASL+
Sbjct: 2478 ESETTASLV 2486



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.279   0.0942    0.185 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 226,547
Number of extensions: 338
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)