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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_937791                                                              17    3301
XP_005269585                                                           17    3301
NP_149985                                                              17    4312
NP_116115                                                              16    5631
XP_006712867                                                           16    5631
XP_005246971                                                           16    5631
XP_006712866                                                           16    5631
XP_005246970                                                           16    5631
NP_000264                                                              15    9605
NP_056475                                                              15    9605
NP_000892                                                              15    9605
NP_001159576                                                           15    9605
XP_005274599                                                           15    9605
XP_005274598                                                           15    9605
XP_006714285                                                           15    9605
XP_005263071                                                           15    9605

>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESLVIYMES 9
            E+LVIYMES
Sbjct: 1102 EALVIYMES 1110



>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    ESLVIYMES 9
            E+LVIYMES
Sbjct: 1102 EALVIYMES 1110



>NP_937791
          Length = 756

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ESLVIYME 8
           ESL+++ME
Sbjct: 631 ESLIVFME 638



>XP_005269585
          Length = 704

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   ESLVIYME 8
           ESL+++ME
Sbjct: 631 ESLIVFME 638



>NP_149985
          Length = 549

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           ESLVI+ ES
Sbjct: 349 ESLVIFEES 357



>NP_116115
          Length = 762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLVIYMES 9
           SLVIY++S
Sbjct: 495 SLVIYLKS 502



>XP_006712867
          Length = 643

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLVIYMES 9
           SLVIY++S
Sbjct: 350 SLVIYLKS 357



>XP_005246971
          Length = 785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLVIYMES 9
           SLVIY++S
Sbjct: 492 SLVIYLKS 499



>XP_006712866
          Length = 788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLVIYMES 9
           SLVIY++S
Sbjct: 495 SLVIYLKS 502



>XP_005246970
          Length = 788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SLVIYMES 9
           SLVIY++S
Sbjct: 495 SLVIYLKS 502



>NP_000264
          Length = 404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ESLVIYME 8
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271



>NP_056475
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           +SL+ YME+
Sbjct: 241 QSLIKYMET 249



>NP_000892
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           ES+ +YM+S
Sbjct: 103 ESMGLYMDS 111



>NP_001159576
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           ES+ +YM+S
Sbjct: 103 ESMGLYMDS 111



>XP_005274599
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ESLVIYME 8
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271



>XP_005274598
          Length = 386

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ESLVIYME 8
           ESL+ Y+E
Sbjct: 264 ESLLFYLE 271



>XP_006714285
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           ES+ +YM+S
Sbjct: 103 ESMGLYMDS 111



>XP_005263071
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ESLVIYMES 9
           ES+ +YM+S
Sbjct: 103 ESMGLYMDS 111



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.126    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 353,907
Number of extensions: 436
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)