Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              18    1135
XP_005266703                                                           18    1135
NP_114429                                                              17    3301
NP_689846                                                              17    4312
NP_001018123                                                           17    4312
NP_073609                                                              16    7355
XP_006718339                                                           16    7355
XP_006718338                                                           16    7355
XP_006718337                                                           16    7355
XP_006718336                                                           16    7355
NP_064627                                                              15    9605
NP_001020029                                                           15    9605
NP_001154976                                                           15    9605
NP_733844                                                              15    9605
NP_008877                                                              15    9605
NP_003739                                                              15    9605
NP_001252541                                                           15    9605
NP_057368                                                              15    9605
NP_996882                                                              15    9605
NP_694539                                                              15    9605
NP_005874                                                              15    9605
NP_066022                                                              15    9605
NP_000338                                                              15    9605
NP_003119                                                              15    9605
NP_001129101                                                           15    9605
XP_006723369                                                           15    9605
XP_006722673                                                           15    9605
XP_006722672                                                           15    9605
XP_006722671                                                           15    9605
XP_006722670                                                           15    9605
XP_005259532                                                           15    9605
XP_006720304                                                           15    9605
XP_005268080                                                           15    9605
XP_005274250                                                           15    9605
XP_006718734                                                           15    9605
XP_006718733                                                           15    9605
XP_005274249                                                           15    9605
XP_006713327                                                           15    9605
XP_006713325                                                           15    9605
XP_006713324                                                           15    9605
XP_005265385                                                           15    9605
XP_006712150                                                           15    9605
XP_005264574                                                           15    9605
>NP_443179
          Length = 2170

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    ESALADRRE 9
            ESALAD RE
Sbjct: 1304 ESALADSRE 1312
>XP_005266703
          Length = 1501

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ESALADRRE 9
           ESALAD RE
Sbjct: 635 ESALADSRE 643
>NP_114429
          Length = 538

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ESALADRRE 9
           E A+AD+RE
Sbjct: 305 EEAIADQRE 313
>NP_689846
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SALADRRE 9
           +AL+DRRE
Sbjct: 800 AALSDRRE 807
>NP_001018123
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   SALADRRE 9
           +AL+DRRE
Sbjct: 800 AALSDRRE 807
>NP_073609
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           ESALAD R
Sbjct: 168 ESALADCR 175
>XP_006718339
          Length = 546

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           ESALAD R
Sbjct: 168 ESALADCR 175
>XP_006718338
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           ESALAD R
Sbjct: 168 ESALADCR 175
>XP_006718337
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           ESALAD R
Sbjct: 168 ESALADCR 175
>XP_006718336
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           ESALAD R
Sbjct: 168 ESALADCR 175
>NP_064627
          Length = 1388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SALADRRE 9
            SAL DR E
Sbjct: 1021 SALVDREE 1028
>NP_001020029
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>NP_001154976
          Length = 503

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  ESALADRRE 9
          E+ALA R+E
Sbjct: 47 EAALAARKE 55
>NP_733844
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ESALADRRE 9
           E+ALA R+E
Sbjct: 107 EAALAARKE 115
>NP_008877
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 50 ALADERE 56
>NP_003739
          Length = 563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ESALADRRE 9
           E+ALA R+E
Sbjct: 107 EAALAARKE 115
>NP_001252541
          Length = 2371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 586 AALASRRE 593
>NP_057368
          Length = 2376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 586 AALASRRE 593
>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 586 AALASRRE 593
>NP_694539
          Length = 769

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           E ALA+RR
Sbjct: 542 EEALANRR 549
>NP_005874
          Length = 2303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 948 AALASRRE 955
>NP_066022
          Length = 2564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 54 ALADERE 60
>NP_000338
          Length = 2137

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>NP_003119
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>NP_001129101
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ESALADRR 8
           E ALA+RR
Sbjct: 542 EEALANRR 549
>XP_006723369
          Length = 2394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 54 ALADERE 60
>XP_006722673
          Length = 2302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 947 AALASRRE 954
>XP_006722672
          Length = 2303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 948 AALASRRE 955
>XP_006722671
          Length = 2303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 948 AALASRRE 955
>XP_006722670
          Length = 2326

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 971 AALASRRE 978
>XP_005259532
          Length = 2327

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SALADRRE 9
           +ALA RRE
Sbjct: 972 AALASRRE 979
>XP_006720304
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>XP_005268080
          Length = 2328

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>XP_005274250
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 50 ALADERE 56
>XP_006718734
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 50 ALADERE 56
>XP_006718733
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 50 ALADERE 56
>XP_005274249
          Length = 2390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 50 ALADERE 56
>XP_006713327
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SALADRRE 9
           SAL DR E
Sbjct: 656 SALVDREE 663
>XP_006713325
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SALADRRE 9
            SAL DR E
Sbjct: 1021 SALVDREE 1028
>XP_006713324
          Length = 1285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    SALADRRE 9
            SAL DR E
Sbjct: 1021 SALVDREE 1028
>XP_005265385
          Length = 1291

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   SALADRRE 9
           SAL DR E
Sbjct: 924 SALVDREE 931
>XP_006712150
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
>XP_005264574
          Length = 2364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  ALADRRE 9
          ALAD RE
Sbjct: 47 ALADERE 53
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.303    0.118    0.278 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 340,246
Number of extensions: 300
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)

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