Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              20     390
XP_006718908                                                           20     390
XP_005271621                                                           20     390
XP_006718907                                                           20     390
XP_006718906                                                           20     390
XP_005271619                                                           20     390
XP_005271618                                                           20     390
NP_001193856                                                           17    4312
XP_006715067                                                           17    4312
NP_055134                                                              16    7355
NP_004810                                                              16    7355
NP_525124                                                              16    7355
NP_001161086                                                           16    7355
NP_057417                                                              16    7355
XP_005259343                                                           16    7355
XP_005255284                                                           16    7355
XP_005255283                                                           16    7355
XP_006720936                                                           16    7355
XP_006720935                                                           16    7355
XP_005255282                                                           16    7355
XP_005250769                                                           16    7355
XP_005250768                                                           16    7355
XP_005250767                                                           16    7355
NP_001120913                                                           16    7355
NP_001175                                                              15    9605
NP_510868                                                              15    9605
>NP_000042
          Length = 3056

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 2221 EPIMALRTV 2229
>XP_006718908
          Length = 1708

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EPMMALRTV 9
           EP+MALRTV
Sbjct: 873 EPIMALRTV 881
>XP_005271621
          Length = 2708

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 1873 EPIMALRTV 1881
>XP_006718907
          Length = 3050

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 2215 EPIMALRTV 2223
>XP_006718906
          Length = 3056

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 2221 EPIMALRTV 2229
>XP_005271619
          Length = 3056

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 2221 EPIMALRTV 2229
>XP_005271618
          Length = 3056

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    EPMMALRTV 9
            EP+MALRTV
Sbjct: 2221 EPIMALRTV 2229
>NP_001193856
          Length = 4707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EPMMALRTV 9
            EPMM  RT+
Sbjct: 2707 EPMMFQRTI 2715
>XP_006715067
          Length = 4707

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    EPMMALRTV 9
            EPMM  RT+
Sbjct: 2707 EPMMFQRTI 2715
>NP_055134
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           EP+ A RTV
Sbjct: 236 EPLWASRTV 244
>NP_004810
          Length = 1274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    PMMALRTV 9
            PM+ +RTV
Sbjct: 995  PMLLMRTV 1002
>NP_525124
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           E  MALRT+
Sbjct: 419 ETWMALRTI 427
>NP_001161086
          Length = 910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           EP+ A RTV
Sbjct: 236 EPLWASRTV 244
>NP_057417
          Length = 2752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2216 PLMSLRT 2222
>XP_005259343
          Length = 1274

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    PMMALRTV 9
            PM+ +RTV
Sbjct: 995  PMLLMRTV 1002
>XP_005255284
          Length = 2581

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2216 PLMSLRT 2222
>XP_005255283
          Length = 2751

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2216 PLMSLRT 2222
>XP_006720936
          Length = 2751

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2215 PLMSLRT 2221
>XP_006720935
          Length = 2752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2216 PLMSLRT 2222
>XP_005255282
          Length = 2752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    PMMALRT 8
            P+M+LRT
Sbjct: 2216 PLMSLRT 2222
>XP_005250769
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           E  MALRT+
Sbjct: 419 ETWMALRTI 427
>XP_005250768
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           E  MALRT+
Sbjct: 419 ETWMALRTI 427
>XP_005250767
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           E  MALRT+
Sbjct: 419 ETWMALRTI 427
>NP_001120913
          Length = 436

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EPMMALRTV 9
           E  MALRT+
Sbjct: 419 ETWMALRTI 427
>NP_001175
          Length = 2644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EPMMALR 7
            EP++ALR
Sbjct: 1898 EPILALR 1904
>NP_510868
          Length = 314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   PMMALRTV 9
           PM  LRTV
Sbjct: 286 PMTPLRTV 293
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.130    0.354 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 376,751
Number of extensions: 438
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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