Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001155973                                                           21     229
NP_055903                                                              21     229
NP_001166923                                                           18    1935
NP_001166924                                                           18    1935
NP_005644                                                              18    1935
NP_002433                                                              17    2528
XP_006719467                                                           17    2528
XP_006719466                                                           17    2528
NP_001070993                                                           17    4312
NP_071930                                                              17    4312
XP_005265457                                                           17    4312
NP_001070994                                                           17    4312
NP_511042                                                              16    7355
NP_004927                                                              16    7355
NP_001263397                                                           16    7355
NP_001469                                                              16    7355
XP_006719394                                                           16    7355
XP_005268830                                                           16    7355
NP_001243143                                                           15    9605
NP_689509                                                              15    9605
NP_001243142                                                           15    9605
NP_689957                                                              15    9605
NP_001367                                                              15    9605
NP_000693                                                              15    9605
NP_001006658                                                           15    9605
NP_065830                                                              15    9605
NP_001153705                                                           15    9605
NP_000692                                                              15    9605
NP_001153706                                                           15    9605
NP_001035262                                                           15    9605
NP_001035263                                                           15    9605
NP_000861                                                              15    9605
NP_001035259                                                           15    9605
NP_055645                                                              15    9605
NP_955525                                                              15    9605
NP_001273339                                                           15    9605
NP_001271272                                                           15    9605
NP_001271270                                                           15    9605
NP_072174                                                              15    9605
NP_001890                                                              15    9605
NP_009201                                                              15    9605
XP_005259010                                                           15    9605
XP_006712784                                                           15    9605
XP_006710718                                                           15    9605
>NP_001155973
          Length = 1833

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           ENK MPFGM
Sbjct: 390 ENKGMPFGM 398
>NP_055903
          Length = 1723

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           ENK MPFGM
Sbjct: 390 ENKGMPFGM 398
>NP_001166923
          Length = 501

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  ENKVMPF 7
          ENK++PF
Sbjct: 61 ENKILPF 67
>NP_001166924
          Length = 450

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  ENKVMPF 7
          ENK++PF
Sbjct: 61 ENKILPF 67
>NP_005644
          Length = 502

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  ENKVMPF 7
          ENK++PF
Sbjct: 61 ENKILPF 67
>NP_002433
          Length = 362

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NKVMPFGM 9
           ++VMP+GM
Sbjct: 200 SRVMPYGM 207
>XP_006719467
          Length = 224

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  NKVMPFGM 9
          ++VMP+GM
Sbjct: 62 SRVMPYGM 69
>XP_006719466
          Length = 351

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   NKVMPFGM 9
           ++VMP+GM
Sbjct: 200 SRVMPYGM 207
>NP_001070993
          Length = 650

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENKVMPFGM 9
           ENK + FGM
Sbjct: 217 ENKKVKFGM 225
>NP_071930
          Length = 538

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENKVMPFGM 9
           ENK + FGM
Sbjct: 217 ENKKVKFGM 225
>XP_005265457
          Length = 625

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENKVMPFGM 9
           ENK + FGM
Sbjct: 217 ENKKVKFGM 225
>NP_001070994
          Length = 598

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ENKVMPFGM 9
           ENK + FGM
Sbjct: 217 ENKKVKFGM 225
>NP_511042
          Length = 78

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  ENKVMPFG 8
          ENK MP G
Sbjct: 4  ENKGMPSG 11
>NP_004927
          Length = 138

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  ENKVMPFG 8
          ENK MP G
Sbjct: 4  ENKGMPSG 11
>NP_001263397
          Length = 478

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ENKVMPFG 8
           E+ +MPFG
Sbjct: 271 EHYLMPFG 278
>NP_001469
          Length = 533

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ENKVMPFG 8
           E+ +MPFG
Sbjct: 326 EHYLMPFG 333
>XP_006719394
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ENKVMPFG 8
           E+ +MPFG
Sbjct: 326 EHYLMPFG 333
>XP_005268830
          Length = 544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   ENKVMPFG 8
           E+ +MPFG
Sbjct: 326 EHYLMPFG 333
>NP_001243143
          Length = 1026

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 953 KNKILIFGL 961
>NP_689509
          Length = 1013

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 940 KNKILIFGL 948
>NP_001243142
          Length = 1024

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 951 KNKILIFGL 959
>NP_689957
          Length = 2213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1   ENKVMPF 7
           ENK  PF
Sbjct: 270 ENKTSPF 276
>NP_001367
          Length = 4646

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2    NKVMPF 7
            NK++PF
Sbjct: 2067 NKIVPF 2072
>NP_000693
          Length = 1020

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 947 KNKILIFGL 955
>NP_001006658
          Length = 1181

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           ++KV+ FGM
Sbjct: 167 DSKVLLFGM 175
>NP_065830
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           ++KV+ FGM
Sbjct: 167 DSKVLLFGM 175
>NP_001153705
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 950 KNKILIFGL 958
>NP_000692
          Length = 1023

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 950 KNKILIFGL 958
>NP_001153706
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 919 KNKILIFGL 927
>NP_001035262
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_001035263
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_000861
          Length = 388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_001035259
          Length = 387

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_055645
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   ENKVMP 6
           ENK MP
Sbjct: 339 ENKAMP 344
>NP_955525
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_001273339
          Length = 411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4  VMPFG 8
          VMPFG
Sbjct: 73 VMPFG 77
>NP_001271272
          Length = 338

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  ENKVMP 6
          ENKV+P
Sbjct: 31 ENKVVP 36
>NP_001271270
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ENKVMP 6
           ENKV+P
Sbjct: 316 ENKVVP 321
>NP_072174
          Length = 1735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ENKVMPFG 8
           E+K++P G
Sbjct: 151 EDKIVPIG 158
>NP_001890
          Length = 141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  ENKVMPFGM 9
          EN+++P G+
Sbjct: 26 ENRIIPGGI 34
>NP_009201
          Length = 570

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   ENKVMP 6
           ENKV+P
Sbjct: 263 ENKVVP 268
>XP_005259010
          Length = 1261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 940 KNKILIFGL 948
>XP_006712784
          Length = 1784

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  ENKVMPFG 8
          E+K++P G
Sbjct: 55 EDKIVPIG 62
>XP_006710718
          Length = 992

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   ENKVMPFGM 9
           +NK++ FG+
Sbjct: 919 KNKILIFGL 927
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.139    0.424 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 663,771
Number of extensions: 1462
Number of successful extensions: 44
Number of sequences better than 10000.0: 44
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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