Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000258                                                              23      60
NP_001035957                                                           23      60
XP_006721991                                                           23      60
XP_006721990                                                           23      60
XP_006721989                                                           23      60
XP_005258041                                                           23      60
XP_006721988                                                           23      60
XP_006721987                                                           23      60
XP_006721986                                                           23      60
XP_005258040                                                           23      60
XP_006721985                                                           23      60
XP_006720951                                                           17    2528
XP_006720950                                                           17    2528
NP_036500                                                              17    2528
NP_055637                                                              17    3301
XP_005263436                                                           17    3301
XP_005263435                                                           17    3301
NP_005898                                                              17    4312
NP_057340                                                              17    4312
XP_005267043                                                           17    4312
NP_008851                                                              16    7355
NP_001189364                                                           16    7355
NP_001159436                                                           16    7355
NP_001159435                                                           16    7355
NP_705839                                                              16    7355
NP_001154926                                                           16    7355
NP_001154924                                                           16    7355
NP_001269399                                                           16    7355
NP_001241657                                                           16    7355
NP_001241658                                                           16    7355
NP_009008                                                              16    7355
NP_001241656                                                           16    7355
NP_001241655                                                           16    7355
NP_001121598                                                           16    7355
NP_055434                                                              16    7355
NP_757374                                                              16    7355
NP_001171618                                                           16    7355
NP_001254                                                              16    7355
NP_000750                                                              16    7355
NP_757373                                                              16    7355
XP_005267350                                                           16    7355
XP_006718529                                                           16    7355
XP_006718528                                                           16    7355
XP_006718527                                                           16    7355
XP_005262744                                                           16    7355
NP_112165                                                              15    9605
NP_001170977                                                           15    9605
NP_056377                                                              15    9605
NP_006032                                                              15    9605
NP_001170978                                                           15    9605
NP_001628                                                              15    9605
NP_078928                                                              15    9605
NP_001186464                                                           15    9605
NP_061328                                                              15    9605
XP_005255036                                                           15    9605
NP_277040                                                              15    9605
YP_003024026                                                           15    9605
>NP_000258
          Length = 2818

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 688 EVALYMFLW 696
>NP_001035957
          Length = 2839

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 688 EVALYMFLW 696
>XP_006721991
          Length = 1632

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_006721990
          Length = 2823

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_006721989
          Length = 2823

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_005258041
          Length = 2836

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 688 EVALYMFLW 696
>XP_006721988
          Length = 2846

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_006721987
          Length = 2849

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_006721986
          Length = 2854

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 685 EVALYMFLW 693
>XP_005258040
          Length = 2857

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 688 EVALYMFLW 696
>XP_006721985
          Length = 2867

 Score = 22.7 bits (47), Expect =    60
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   EMALYMFLW 9
           E+ALYMFLW
Sbjct: 698 EVALYMFLW 706
>XP_006720951
          Length = 312

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           LY+FLW
Sbjct: 101 LYVFLW 106
>XP_006720950
          Length = 312

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           LY+FLW
Sbjct: 101 LYVFLW 106
>NP_036500
          Length = 312

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           LY+FLW
Sbjct: 101 LYVFLW 106
>NP_055637
          Length = 1032

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           L+MFLW
Sbjct: 925 LHMFLW 930
>XP_005263436
          Length = 1033

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           L+MFLW
Sbjct: 926 LHMFLW 931
>XP_005263435
          Length = 1033

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           L+MFLW
Sbjct: 926 LHMFLW 931
>NP_005898
          Length = 653

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MALYMFLW 9
           M  YM++W
Sbjct: 549 METYMYMW 556
>NP_057340
          Length = 515

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  MALYMFLW 9
          +AL+ FLW
Sbjct: 13 LALFGFLW 20
>XP_005267043
          Length = 736

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MALYMFLW 9
           M  YM++W
Sbjct: 632 METYMYMW 639
>NP_008851
          Length = 1998

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMALYMFLW 9
            E +LYM+L+
Sbjct: 1443 EESLYMYLY 1451
>NP_001189364
          Length = 2009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMALYMFLW 9
            E +LYM+L+
Sbjct: 1454 EESLYMYLY 1462
>NP_001159436
          Length = 1981

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMALYMFLW 9
            E +LYM+L+
Sbjct: 1426 EESLYMYLY 1434
>NP_001159435
          Length = 2009

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    EMALYMFLW 9
            E +LYM+L+
Sbjct: 1454 EESLYMYLY 1462
>NP_705839
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 40 SIYMFNW 46
>NP_001154926
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 57 SIYMFNW 63
>NP_001154924
          Length = 242

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 40 SIYMFNW 46
>NP_001269399
          Length = 956

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   MALYMFLW 9
           MA+  FLW
Sbjct: 822 MAMLEFLW 829
>NP_001241657
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241658
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_009008
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241656
          Length = 149

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001241655
          Length = 135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_001121598
          Length = 144

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>NP_055434
          Length = 956

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   MALYMFLW 9
           MA+  FLW
Sbjct: 822 MAMLEFLW 829
>NP_757374
          Length = 171

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  MALYMFLW 9
          MAL + LW
Sbjct: 13 MALQLLLW 20
>NP_001171618
          Length = 168

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  MALYMFLW 9
          MAL + LW
Sbjct: 13 MALQLLLW 20
>NP_001254
          Length = 461

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2   MALYMFLW 9
           +  YMF W
Sbjct: 209 LQFYMFAW 216
>NP_000750
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  MALYMFLW 9
          MAL + LW
Sbjct: 13 MALQLLLW 20
>NP_757373
          Length = 204

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  MALYMFLW 9
          MAL + LW
Sbjct: 13 MALQLLLW 20
>XP_005267350
          Length = 159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+Y F+
Sbjct: 77 EMAIYEFI 84
>XP_006718529
          Length = 135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 40 SIYMFNW 46
>XP_006718528
          Length = 161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 40 SIYMFNW 46
>XP_006718527
          Length = 263

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3  ALYMFLW 9
          ++YMF W
Sbjct: 40 SIYMFNW 46
>XP_005262744
          Length = 421

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 5/8 (62%)

Query: 2   MALYMFLW 9
           +  YMF W
Sbjct: 209 LQFYMFAW 216
>NP_112165
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/6 (50%), Positives = 6/6 (100%)

Query: 4   LYMFLW 9
           LY+++W
Sbjct: 101 LYVYMW 106
>NP_001170977
          Length = 688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EMALYMFLW 9
           E ++Y+ LW
Sbjct: 316 EKSIYLRLW 324
>NP_056377
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  EMALYMFL 8
          EMA+ MFL
Sbjct: 43 EMAVTMFL 50
>NP_006032
          Length = 390

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  LYMFLW 9
          LYMFL+
Sbjct: 44 LYMFLY 49
>NP_001170978
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EMALYMFLW 9
           E ++Y+ LW
Sbjct: 284 EKSIYLRLW 292
>NP_001628
          Length = 575

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EMALYMFLW 9
           E ++Y+ LW
Sbjct: 316 EKSIYLRLW 324
>NP_078928
          Length = 2015

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   EMALYMFLW 9
           + ALY+ +W
Sbjct: 716 DKALYVVVW 724
>NP_001186464
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   ALYMFLW 9
           AL+M +W
Sbjct: 139 ALFMVIW 145
>NP_061328
          Length = 129

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 3   ALYMFLW 9
           AL+M +W
Sbjct: 113 ALFMVIW 119
>XP_005255036
          Length = 1310

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  EMALYMFLW 9
          + ALY+ +W
Sbjct: 11 DKALYVVVW 19
>NP_277040
          Length = 397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   LYMFLW 9
           LY+ LW
Sbjct: 156 LYLLLW 161
>YP_003024026
          Length = 318

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 2   MALYMFLW 9
           +A+Y  LW
Sbjct: 111 LAVYSILW 118
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.343    0.142    0.569 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 387,224
Number of extensions: 690
Number of successful extensions: 57
Number of sequences better than 10000.0: 57
Number of HSP's gapped: 57
Number of HSP's successfully gapped: 57
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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