Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              20     299
NP_066124                                                              20     299
XP_006717999                                                           20     299
NP_001171940                                                           17    2528
NP_689512                                                              17    2528
XP_005261969                                                           17    2528
NP_775113                                                              17    4312
NP_077320                                                              17    4312
NP_775111                                                              17    4312
NP_775110                                                              17    4312
NP_775112                                                              17    4312
NP_000061                                                              17    4312
NP_055945                                                              16    5631
NP_036330                                                              16    5631
XP_005246503                                                           16    5631
XP_006712464                                                           16    5631
XP_006712463                                                           16    5631
XP_006712462                                                           16    5631
XP_006712461                                                           16    5631
NP_036252                                                              16    7355
NP_057209                                                              16    7355
NP_001107033                                                           16    7355
XP_005249034                                                           16    7355
XP_005249033                                                           16    7355
XP_006714654                                                           16    7355
XP_006714653                                                           16    7355
XP_006714652                                                           16    7355
NP_001155899                                                           15    9605
NP_000359                                                              15    9605
NP_001155898                                                           15    9605
NP_006747                                                              15    9605
NP_001269848                                                           15    9605
NP_001740                                                              15    9605
NP_958845                                                              15    9605
XP_005259354                                                           15    9605
XP_005259353                                                           15    9605
XP_005259352                                                           15    9605
XP_006717334                                                           15    9605
XP_005272268                                                           15    9605
XP_006716530                                                           15    9605
XP_005251248                                                           15    9605
NP_001003962                                                           15    9605
>NP_065681
          Length = 1072

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           ELCRT+IAA
Sbjct: 632 ELCRTVIAA 640
>NP_066124
          Length = 1114

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           ELCRT+IAA
Sbjct: 632 ELCRTVIAA 640
>XP_006717999
          Length = 1207

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           ELCRT+IAA
Sbjct: 725 ELCRTVIAA 733
>NP_001171940
          Length = 606

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           E+CR+M+A+
Sbjct: 549 EVCRSMLAS 557
>NP_689512
          Length = 605

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           E+CR+M+A+
Sbjct: 548 EVCRSMLAS 556
>XP_005261969
          Length = 579

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   ELCRTMIAA 9
           E+CR+M+A+
Sbjct: 522 EVCRSMLAS 530
>NP_775113
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ELCRTMIA 8
          E CR+MIA
Sbjct: 30 ESCRSMIA 37
>NP_077320
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           E CR+MIA
Sbjct: 689 ESCRSMIA 696
>NP_775111
          Length = 309

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           E CR+MIA
Sbjct: 183 ESCRSMIA 190
>NP_775110
          Length = 729

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           E CR+MIA
Sbjct: 603 ESCRSMIA 610
>NP_775112
          Length = 156

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  ELCRTMIA 8
          E CR+MIA
Sbjct: 30 ESCRSMIA 37
>NP_000061
          Length = 821

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           E CR+MIA
Sbjct: 695 ESCRSMIA 702
>NP_055945
          Length = 1266

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    ELCRTM 6
            ELC+TM
Sbjct: 1031 ELCKTM 1036
>NP_036330
          Length = 217

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  ELCRTMIA 8
          E CR MIA
Sbjct: 92 ETCRIMIA 99
>XP_005246503
          Length = 233

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  ELCRTMIA 8
          E CR MIA
Sbjct: 92 ETCRIMIA 99
>XP_006712464
          Length = 243

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 118 ETCRIMIA 125
>XP_006712463
          Length = 248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 107 ETCRIMIA 114
>XP_006712462
          Length = 249

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 118 ETCRIMIA 125
>XP_006712461
          Length = 259

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 118 ETCRIMIA 125
>NP_036252
          Length = 639

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CRT+ A
Sbjct: 272 EYCRTLFA 279
>NP_057209
          Length = 435

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LCRTMIA 8
           LCRT+I+
Sbjct: 411 LCRTVIS 417
>NP_001107033
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LCRTMIA 8
           LCRT+I+
Sbjct: 472 LCRTIIS 478
>XP_005249034
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CRT+ A
Sbjct: 272 EYCRTLFA 279
>XP_005249033
          Length = 635

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CRT+ A
Sbjct: 268 EYCRTLFA 275
>XP_006714654
          Length = 537

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LCRTMIA 8
           LCRT+I+
Sbjct: 472 LCRTIIS 478
>XP_006714653
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LCRTMIA 8
           LCRT+I+
Sbjct: 472 LCRTIIS 478
>XP_006714652
          Length = 592

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   LCRTMIA 8
           LCRT+I+
Sbjct: 472 LCRTIIS 478
>NP_001155899
          Length = 1113

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 750 EDCRNMIA 757
>NP_000359
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 801 EDCRNMIA 808
>NP_001155898
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 800 EDCRNMIA 807
>NP_006747
          Length = 301

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   CRTMIAA 9
           CR M+AA
Sbjct: 144 CREMLAA 150
>NP_001269848
          Length = 876

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LCRTMIAA 9
           LCRT+  A
Sbjct: 852 LCRTLAVA 859
>NP_001740
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           + CR+M+A
Sbjct: 142 DTCRSMVA 149
>NP_958845
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   CRTMIAA 9
           CR M+AA
Sbjct: 123 CREMLAA 129
>XP_005259354
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  ELCRTMIA 8
          + CR+M+A
Sbjct: 30 DTCRSMVA 37
>XP_005259353
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           + CR+M+A
Sbjct: 142 DTCRSMVA 149
>XP_005259352
          Length = 322

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           + CR+M+A
Sbjct: 142 DTCRSMVA 149
>XP_006717334
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 801 EDCRNMIA 808
>XP_005272268
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   ELCRTMIA 8
           E CR MIA
Sbjct: 801 EDCRNMIA 808
>XP_006716530
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   CRTMIAA 9
           CR M+AA
Sbjct: 97  CREMLAA 103
>XP_005251248
          Length = 848

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   LCRTMIAA 9
           LCRT+  A
Sbjct: 824 LCRTLAVA 831
>NP_001003962
          Length = 268

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   ELCRTMIA 8
           + CR+M+A
Sbjct: 142 DTCRSMVA 149
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.132    0.399 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 522,306
Number of extensions: 308
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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