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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000042                                                              18    1482
XP_005271621                                                           18    1482
XP_006718907                                                           18    1482
XP_006718906                                                           18    1482
XP_005271619                                                           18    1482
XP_005271618                                                           18    1482
NP_000284                                                              17    2528
NP_001027005                                                           17    2528
XP_005256041                                                           17    2528
XP_005256040                                                           17    2528
NP_001278261                                                           15    9605
NP_001034553                                                           15    9605

>NP_000042
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 1342 EIVVELLM 1349



>XP_005271621
          Length = 2708

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 994  EIVVELLM 1001



>XP_006718907
          Length = 3050

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 1336 EIVVELLM 1343



>XP_006718906
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 1342 EIVVELLM 1349



>XP_005271619
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 1342 EIVVELLM 1349



>XP_005271618
          Length = 3056

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    EIVVELLM 8
            EIVVELLM
Sbjct: 1342 EIVVELLM 1349



>NP_000284
          Length = 1093

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    EIVVELLMM 9
            +IVVELLM+
Sbjct: 997  QIVVELLMV 1005



>NP_001027005
          Length = 1086

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EIVVELLMM 9
           +IVVELLM+
Sbjct: 990 QIVVELLMV 998



>XP_005256041
          Length = 1086

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   EIVVELLMM 9
           +IVVELLM+
Sbjct: 990 QIVVELLMV 998



>XP_005256040
          Length = 1093

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    EIVVELLMM 9
            +IVVELLM+
Sbjct: 997  QIVVELLMV 1005



>NP_001278261
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EIVVELLM 8
           E+VV LLM
Sbjct: 298 EVVVALLM 305



>NP_001034553
          Length = 1323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EIVVELLM 8
           E+VV LLM
Sbjct: 780 EVVVALLM 787



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.150    0.346 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 266,702
Number of extensions: 318
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)