Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              20     390
XP_006713721                                                           20     390
NP_003045                                                              18    1482
NP_062818                                                              17    4312
NP_001182212                                                           17    4312
NP_001136277                                                           16    5631
NP_005620                                                              16    5631
NP_068566                                                              16    5631
NP_722580                                                              16    5631
NP_001010898                                                           16    5631
XP_005244867                                                           16    5631
XP_006711056                                                           16    5631
XP_006710706                                                           16    5631
XP_006710057                                                           16    5631
NP_008914                                                              16    5631
NP_001001323                                                           16    7355
NP_001673                                                              16    7355
NP_001001344                                                           16    7355
NP_068768                                                              16    7355
NP_001675                                                              16    7355
NP_001001396                                                           16    7355
NP_001001331                                                           16    7355
NP_071394                                                              16    7355
NP_060527                                                              16    7355
NP_877499                                                              16    7355
NP_001177410                                                           16    7355
NP_001093591                                                           16    7355
NP_001093590                                                           16    7355
NP_001674                                                              16    7355
XP_005274750                                                           16    7355
XP_005274749                                                           16    7355
XP_005274748                                                           16    7355
XP_005274747                                                           16    7355
XP_006724886                                                           16    7355
XP_005274746                                                           16    7355
XP_005268976                                                           16    7355
XP_006713245                                                           16    7355
XP_006713244                                                           16    7355
XP_006713243                                                           16    7355
XP_006713242                                                           16    7355
XP_006713241                                                           16    7355
XP_006713240                                                           16    7355
XP_006713239                                                           16    7355
XP_005265236                                                           16    7355
XP_006713238                                                           16    7355
NP_001032401                                                           16    7355
NP_055508                                                              16    7355
NP_004512                                                              15    9605
NP_009162                                                              15    9605
NP_003033                                                              15    9605
NP_775735                                                              15    9605
NP_059965                                                              15    9605
XP_005260502                                                           15    9605
XP_006722429                                                           15    9605
XP_006722428                                                           15    9605
XP_006722427                                                           15    9605
XP_005258225                                                           15    9605
XP_006722425                                                           15    9605
XP_005258223                                                           15    9605
XP_006713369                                                           15    9605
XP_005265468                                                           15    9605
XP_005265467                                                           15    9605
NP_001185498                                                           15    9605
NP_001685                                                              15    9605
>NP_006209
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           EIGFAIGM
Sbjct: 116 EIGFAIGM 123
>XP_006713721
          Length = 1068

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           EIGFAIGM
Sbjct: 116 EIGFAIGM 123
>NP_003045
          Length = 514

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   IGFAIGML 9
           +GFAIGM+
Sbjct: 391 VGFAIGMV 398
>NP_062818
          Length = 702

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IGFAIGML 9
           IGFA+G L
Sbjct: 222 IGFALGSL 229
>NP_001182212
          Length = 321

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  EIGFAIGML 9
          E+G AIG L
Sbjct: 18 ELGIAIGFL 26
>NP_001136277
          Length = 625

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +GFA+G+
Sbjct: 66 VGFAVGL 72
>NP_005620
          Length = 635

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +GFA+G+
Sbjct: 66 VGFAVGL 72
>NP_068566
          Length = 790

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EIGFAIGM 8
           E+G A+GM
Sbjct: 339 ELGHAVGM 346
>NP_722580
          Length = 695

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   IGFAIG 7
           IGFAIG
Sbjct: 517 IGFAIG 522
>NP_001010898
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  EIGFAIGM 8
          +IGF++G+
Sbjct: 73 QIGFSVGL 80
>XP_005244867
          Length = 332

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIGFAIGML 9
           EIG A+G++
Sbjct: 212 EIGHALGLM 220
>XP_006711056
          Length = 389

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIGFAIGML 9
           EIG A+G++
Sbjct: 212 EIGHALGLM 220
>XP_006710706
          Length = 727

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1  EIGFAIGM 8
          +IGF++G+
Sbjct: 73 QIGFSVGL 80
>XP_006710057
          Length = 99

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +GFA+G+
Sbjct: 8  VGFAVGL 14
>NP_008914
          Length = 390

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EIGFAIGML 9
           EIG A+G++
Sbjct: 212 EIGHALGLM 220
>NP_001001323
          Length = 1176

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 809 DVGFAMGI 816
>NP_001673
          Length = 1220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 809 DVGFAMGI 816
>NP_001001344
          Length = 1220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 806 DVGFAMGI 813
>NP_068768
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 806 DVGFAMGI 813
>NP_001675
          Length = 1205

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 797 DVGFAMGI 804
>NP_001001396
          Length = 1170

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 797 DVGFAMGI 804
>NP_001001331
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>NP_071394
          Length = 1339

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EIGFAIGML 9
           EIG A+G L
Sbjct: 705 EIGKAVGFL 713
>NP_060527
          Length = 289

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  EIGFAIGM 8
          ++GF++G+
Sbjct: 74 QVGFSVGL 81
>NP_877499
          Length = 730

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 1  EIGFAIGM 8
          ++GF++G+
Sbjct: 74 QVGFSVGL 81
>NP_001177410
          Length = 1275

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EIGFAIGML 9
           EIG A+G L
Sbjct: 641 EIGKAVGFL 649
>NP_001093591
          Length = 765

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IGFAIGML 9
           +GFA+G L
Sbjct: 438 LGFALGSL 445
>NP_001093590
          Length = 811

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IGFAIGML 9
           +GFA+G L
Sbjct: 484 LGFALGSL 491
>NP_001674
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 787 DVGFAMGI 794
>XP_005274750
          Length = 1159

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 792 DVGFAMGI 799
>XP_005274749
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 806 DVGFAMGI 813
>XP_005274748
          Length = 1206

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 792 DVGFAMGI 799
>XP_005274747
          Length = 1220

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 806 DVGFAMGI 813
>XP_006724886
          Length = 1235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 792 DVGFAMGI 799
>XP_005274746
          Length = 1249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 806 DVGFAMGI 813
>XP_005268976
          Length = 1249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 809 DVGFAMGI 816
>XP_006713245
          Length = 1174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 801 DVGFAMGI 808
>XP_006713244
          Length = 1198

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 787 DVGFAMGI 794
>XP_006713243
          Length = 1199

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>XP_006713242
          Length = 1205

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>XP_006713241
          Length = 1212

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 801 DVGFAMGI 808
>XP_006713240
          Length = 1232

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 821 DVGFAMGI 828
>XP_006713239
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>XP_005265236
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>XP_006713238
          Length = 1243

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EIGFAIGM 8
           ++GFA+G+
Sbjct: 832 DVGFAMGI 839
>NP_001032401
          Length = 736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IGFAIGML 9
           +GFA+G L
Sbjct: 409 LGFALGSL 416
>NP_055508
          Length = 883

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   IGFAIGML 9
           +GFA+G L
Sbjct: 556 LGFALGSL 563
>NP_004512
          Length = 963

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   EIGFAIG 7
           EIG A+G
Sbjct: 564 EIGIAVG 570
>NP_009162
          Length = 642

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          IG+A+G+
Sbjct: 50 IGYAVGL 56
>NP_003033
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +G+AIG+
Sbjct: 58 VGYAIGL 64
>NP_775735
          Length = 623

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>NP_059965
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EIGFAIGM 8
           + GFAIG+
Sbjct: 119 QTGFAIGV 126
>XP_005260502
          Length = 271

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   EIGFAIGM 8
           + GFAIG+
Sbjct: 151 QTGFAIGV 158
>XP_006722429
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_006722428
          Length = 370

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_006722427
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_005258225
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_006722425
          Length = 593

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_005258223
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  EIGFAIGM 8
          E GF IGM
Sbjct: 81 ENGFQIGM 88
>XP_006713369
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +G+AIG+
Sbjct: 58 VGYAIGL 64
>XP_005265468
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +G+AIG+
Sbjct: 58 VGYAIGL 64
>XP_005265467
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 2  IGFAIGM 8
          +G+AIG+
Sbjct: 58 VGYAIGL 64
>NP_001185498
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GFAIGML 9
           GFAIG++
Sbjct: 107 GFAIGIV 113
>NP_001685
          Length = 155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   GFAIGML 9
           GFAIG++
Sbjct: 107 GFAIGIV 113
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.160    0.458 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,089
Number of extensions: 1019
Number of successful extensions: 64
Number of sequences better than 10000.0: 64
Number of HSP's gapped: 64
Number of HSP's successfully gapped: 64
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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