Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     665
NP_061836                                                              17    4312
NP_115997                                                              17    4312
NP_387505                                                              17    4312
XP_006724404                                                           17    4312
XP_006724403                                                           17    4312
XP_005261845                                                           17    4312
XP_005261844                                                           17    4312
XP_005261843                                                           17    4312
XP_005261051                                                           17    4312
NP_079107                                                              16    5631
XP_006716549                                                           16    5631
XP_006716548                                                           16    5631
XP_005251385                                                           16    5631
NP_002370                                                              16    7355
NP_001275896                                                           16    7355
NP_001275894                                                           16    7355
NP_077817                                                              16    7355
NP_001275893                                                           16    7355
XP_005247863                                                           16    7355
NP_001156333                                                           15    9605
NP_001073379                                                           15    9605
NP_057646                                                              15    9605
XP_006719697                                                           15    9605
XP_006719696                                                           15    9605
XP_006719695                                                           15    9605
XP_005269238                                                           15    9605
>NP_005219
          Length = 1210

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EFKKIKVL 8
           EFKKIKVL
Sbjct: 711 EFKKIKVL 718
>NP_061836
          Length = 2320

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK K+LS
Sbjct: 1813 FKKTKILS 1820
>NP_115997
          Length = 2567

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1355 EFKKLKI 1361
>NP_387505
          Length = 2269

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK K+LS
Sbjct: 1813 FKKTKILS 1820
>XP_006724404
          Length = 1442

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1356 EFKKLKI 1362
>XP_006724403
          Length = 2449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1237 EFKKLKI 1243
>XP_005261845
          Length = 2568

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1356 EFKKLKI 1362
>XP_005261844
          Length = 2568

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1356 EFKKLKI 1362
>XP_005261843
          Length = 2568

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    EFKKIKV 7
            EFKK+K+
Sbjct: 1356 EFKKLKI 1362
>XP_005261051
          Length = 2242

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK K+LS
Sbjct: 1735 FKKTKILS 1742
>NP_079107
          Length = 853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKKIKVLS 9
           FKK K+LS
Sbjct: 506 FKKSKILS 513
>XP_006716549
          Length = 646

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKKIKVLS 9
           FKK K+LS
Sbjct: 413 FKKSKILS 420
>XP_006716548
          Length = 739

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKKIKVLS 9
           FKK K+LS
Sbjct: 506 FKKSKILS 513
>XP_005251385
          Length = 760

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKKIKVLS 9
           FKK K+LS
Sbjct: 413 FKKSKILS 420
>NP_002370
          Length = 496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  EFKKIKV 7
          EF+K+KV
Sbjct: 56 EFEKVKV 62
>NP_001275896
          Length = 445

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKIKVLS 9
           KK+KVLS
Sbjct: 438 KKLKVLS 444
>NP_001275894
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKIKVLS 9
           KK+KVLS
Sbjct: 570 KKLKVLS 576
>NP_077817
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKIKVLS 9
           KK+KVLS
Sbjct: 590 KKLKVLS 596
>NP_001275893
          Length = 579

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKIKVLS 9
           KK+KVLS
Sbjct: 572 KKLKVLS 578
>XP_005247863
          Length = 597

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KKIKVLS 9
           KK+KVLS
Sbjct: 590 KKLKVLS 596
>NP_001156333
          Length = 230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   FKKIKVLS 9
           F KI+VLS
Sbjct: 212 FNKIQVLS 219
>NP_001073379
          Length = 1722

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK+ +LS
Sbjct: 1201 FKKLMILS 1208
>NP_057646
          Length = 1049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   EFKKIKVL 8
           +FK++KV+
Sbjct: 419 QFKRLKVI 426
>XP_006719697
          Length = 1658

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK+ +LS
Sbjct: 1137 FKKLMILS 1144
>XP_006719696
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK+ +LS
Sbjct: 1137 FKKLMILS 1144
>XP_006719695
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK+ +LS
Sbjct: 1137 FKKLMILS 1144
>XP_005269238
          Length = 1696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    FKKIKVLS 9
            FKK+ +LS
Sbjct: 1137 FKKLMILS 1144
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.137    0.331 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 384,500
Number of extensions: 490
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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