Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              20     299
XP_005266703                                                           20     299
XP_005265088                                                           18    1135
NP_056281                                                              18    1135
NP_056036                                                              17    4312
XP_005261543                                                           17    4312
XP_005261542                                                           17    4312
XP_006717799                                                           17    4312
XP_006717798                                                           17    4312
NP_777592                                                              17    4312
NP_060119                                                              16    5631
NP_951058                                                              16    5631
NP_006651                                                              16    5631
NP_001242941                                                           16    5631
NP_001139281                                                           16    5631
NP_116115                                                              16    5631
NP_005955                                                              16    5631
NP_001070654                                                           16    5631
NP_079005                                                              16    5631
NP_001243024                                                           16    5631
XP_006723449                                                           16    5631
XP_005256710                                                           16    5631
XP_005256709                                                           16    5631
XP_005256708                                                           16    5631
XP_005246972                                                           16    5631
XP_006712867                                                           16    5631
XP_005246971                                                           16    5631
XP_006712866                                                           16    5631
XP_005246970                                                           16    5631
NP_065954                                                              16    5631
NP_056481                                                              16    7355
NP_115609                                                              16    7355
NP_036551                                                              16    7355
XP_006723838                                                           16    7355
XP_005260450                                                           16    7355
XP_006723837                                                           16    7355
XP_006723836                                                           16    7355
XP_006723835                                                           16    7355
XP_005268184                                                           16    7355
XP_005268183                                                           16    7355
XP_006716233                                                           16    7355
XP_006715523                                                           16    7355
NP_443085                                                              16    7355
NP_000892                                                              15    9605
NP_001159576                                                           15    9605
NP_064581                                                              15    9605
NP_001244025                                                           15    9605
NP_006183                                                              15    9605
XP_006714285                                                           15    9605
XP_005263071                                                           15    9605
>NP_443179
          Length = 2170

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   EDLKYEQS 8
           EDLKYEQS
Sbjct: 735 EDLKYEQS 742
>XP_005266703
          Length = 1501

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1  EDLKYEQS 8
          EDLKYEQS
Sbjct: 66 EDLKYEQS 73
>XP_005265088
          Length = 1694

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EDLKYEQS 8
           ED+KYEQ+
Sbjct: 176 EDIKYEQA 183
>NP_056281
          Length = 1636

 Score = 18.5 bits (36), Expect =  1135
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   EDLKYEQS 8
           ED+KYEQ+
Sbjct: 108 EDIKYEQA 115
>NP_056036
          Length = 1577

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           EDLK+ +SN
Sbjct: 733 EDLKFCESN 741
>XP_005261543
          Length = 231

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DLKYEQSN 9
           DLKYE S+
Sbjct: 199 DLKYETSS 206
>XP_005261542
          Length = 264

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DLKYEQSN 9
           DLKYE S+
Sbjct: 232 DLKYETSS 239
>XP_006717799
          Length = 976

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           EDLK+ +SN
Sbjct: 733 EDLKFCESN 741
>XP_006717798
          Length = 1534

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           EDLK+ +SN
Sbjct: 733 EDLKFCESN 741
>NP_777592
          Length = 507

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   DLKYEQSN 9
           DLKYE S+
Sbjct: 475 DLKYETSS 482
>NP_060119
          Length = 875

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           ++LKY++ N
Sbjct: 660 QELKYDEKN 668
>NP_951058
          Length = 853

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           ++LKY++ N
Sbjct: 660 QELKYDEKN 668
>NP_006651
          Length = 633

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EDLKYEQSN 9
           +D+K E+SN
Sbjct: 280 DDIKLEKSN 288
>NP_001242941
          Length = 2007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1774 EELEEEQSN 1782
>NP_001139281
          Length = 2036

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1801 EELEEEQSN 1809
>NP_116115
          Length = 762

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 441 EDLEYEE 447
>NP_005955
          Length = 1976

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1743 EELEEEQSN 1751
>NP_001070654
          Length = 2003

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1768 EELEEEQSN 1776
>NP_079005
          Length = 1995

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1760 EELEEEQSN 1768
>NP_001243024
          Length = 1985

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1752 EELEEEQSN 1760
>XP_006723449
          Length = 2003

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1768 EELEEEQSN 1776
>XP_005256710
          Length = 1986

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1753 EELEEEQSN 1761
>XP_005256709
          Length = 1997

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1764 EELEEEQSN 1772
>XP_005256708
          Length = 2007

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    EDLKYEQSN 9
            E+L+ EQSN
Sbjct: 1774 EELEEEQSN 1782
>XP_005246972
          Length = 494

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 441 EDLEYEE 447
>XP_006712867
          Length = 643

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 296 EDLEYEE 302
>XP_005246971
          Length = 785

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 438 EDLEYEE 444
>XP_006712866
          Length = 788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 441 EDLEYEE 447
>XP_005246970
          Length = 788

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   EDLKYEQ 7
           EDL+YE+
Sbjct: 441 EDLEYEE 447
>NP_065954
          Length = 922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   EDLKYEQSN 9
           + LKY QSN
Sbjct: 198 DSLKYIQSN 206
>NP_056481
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 322 DDLKYE 327
>NP_115609
          Length = 594

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KYEQSN 9
           KYE+SN
Sbjct: 501 KYEESN 506
>NP_036551
          Length = 210

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDLKYEQS 8
           EDLK EQ+
Sbjct: 118 EDLKKEQN 125
>XP_006723838
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 322 DDLKYE 327
>XP_005260450
          Length = 406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 322 DDLKYE 327
>XP_006723837
          Length = 422

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 338 DDLKYE 343
>XP_006723836
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 350 DDLKYE 355
>XP_006723835
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   EDLKYE 6
           +DLKYE
Sbjct: 350 DDLKYE 355
>XP_005268184
          Length = 495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KYEQSN 9
           KYE+SN
Sbjct: 402 KYEESN 407
>XP_005268183
          Length = 536

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   KYEQSN 9
           KYE+SN
Sbjct: 443 KYEESN 448
>XP_006716233
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DLKYEQSN 9
           DL+YE  N
Sbjct: 362 DLRYEAQN 369
>XP_006715523
          Length = 149

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   EDLKYEQS 8
           EDLK EQ+
Sbjct: 118 EDLKKEQN 125
>NP_443085
          Length = 626

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   DLKYEQSN 9
           DL+YE  N
Sbjct: 362 DLRYEAQN 369
>NP_000892
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   DLKYEQSN 9
           D  YEQ N
Sbjct: 116 DYSYEQQN 123
>NP_001159576
          Length = 867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   DLKYEQSN 9
           D  YEQ N
Sbjct: 116 DYSYEQQN 123
>NP_064581
          Length = 855

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  EDLKYEQ 7
          EDL YE+
Sbjct: 20 EDLPYEE 26
>NP_001244025
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   DLKYEQS 8
           D+KY QS
Sbjct: 348 DIKYTQS 354
>NP_006183
          Length = 534

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   DLKYEQS 8
           D+KY QS
Sbjct: 348 DIKYTQS 354
>XP_006714285
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   DLKYEQSN 9
           D  YEQ N
Sbjct: 116 DYSYEQQN 123
>XP_005263071
          Length = 984

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 2   DLKYEQSN 9
           D  YEQ N
Sbjct: 116 DYSYEQQN 123
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.290    0.116    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 640,601
Number of extensions: 1139
Number of successful extensions: 50
Number of sequences better than 10000.0: 50
Number of HSP's gapped: 50
Number of HSP's successfully gapped: 50
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.8 bits)
S2: 28 (15.4 bits)

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