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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              19     869
XP_006710739                                                           17    2528
XP_005270979                                                           17    2528
NP_056972                                                              17    2528
NP_001191354                                                           17    4312
NP_060163                                                              17    4312
NP_001153068                                                           17    4312
NP_079212                                                              16    5631
NP_150094                                                              15    9605

>NP_005219
          Length = 1210

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   EATSSKANK 9
           EATS KANK
Sbjct: 749 EATSPKANK 757



>XP_006710739
          Length = 560

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ATSSKANK 9
           ATS KANK
Sbjct: 311 ATSDKANK 318



>XP_005270979
          Length = 728

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ATSSKANK 9
           ATS KANK
Sbjct: 311 ATSDKANK 318



>NP_056972
          Length = 728

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   ATSSKANK 9
           ATS KANK
Sbjct: 311 ATSDKANK 318



>NP_001191354
          Length = 1640

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EATSSKANK 9
           EAT  KANK
Sbjct: 334 EATPVKANK 342



>NP_060163
          Length = 503

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EATSSKANK 9
           EAT  KANK
Sbjct: 334 EATPVKANK 342



>NP_001153068
          Length = 281

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   EATSSKANK 9
           EAT  KANK
Sbjct: 105 EATPVKANK 113



>NP_079212
          Length = 513

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ATSSKANK 9
           ATS +ANK
Sbjct: 462 ATSKRANK 469



>NP_150094
          Length = 3564

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    ATSSKAN 8
            ATSSK N
Sbjct: 3380 ATSSKVN 3386



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.266   0.0829    0.175 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 204,989
Number of extensions: 147
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 19 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.3 bits)
S2: 28 (15.4 bits)