Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004295                                                              18    1135
NP_001129157                                                           16    5631
NP_996844                                                              16    5631
XP_005254439                                                           16    5631
XP_006720568                                                           16    5631
XP_005254438                                                           16    5631
NP_002335                                                              16    5631
NP_056190                                                              16    7355
NP_955749                                                              16    7355
NP_598328                                                              15    9605
NP_003169                                                              15    9605
NP_443076                                                              15    9605
NP_008905                                                              15    9605
NP_008886                                                              15    9605
NP_001265108                                                           15    9605
NP_001265106                                                           15    9605
NP_001265105                                                           15    9605
NP_001265103                                                           15    9605
NP_001265102                                                           15    9605
NP_001265100                                                           15    9605
NP_001265099                                                           15    9605
NP_065904                                                              15    9605
NP_056081                                                              15    9605
NP_001247420                                                           15    9605
NP_001265104                                                           15    9605
NP_001265107                                                           15    9605
NP_006409                                                              15    9605
XP_006723819                                                           15    9605
XP_006718031                                                           15    9605
XP_006718030                                                           15    9605
XP_006718029                                                           15    9605
XP_005271882                                                           15    9605
XP_006718028                                                           15    9605
XP_006718027                                                           15    9605
XP_006713376                                                           15    9605
XP_006713375                                                           15    9605
XP_006713374                                                           15    9605
NP_008885                                                              15    9605
>NP_004295
          Length = 1620

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    EALIISKLN 9
            EALIISK N
Sbjct: 1167 EALIISKFN 1175
>NP_001129157
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 431 EALIISK 437
>NP_996844
          Length = 803

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 500 EALIISK 506
>XP_005254439
          Length = 475

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 172 EALIISK 178
>XP_006720568
          Length = 771

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 468 EALIISK 474
>XP_005254438
          Length = 819

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 516 EALIISK 522
>NP_002335
          Length = 864

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   EALIISK 7
           EALIISK
Sbjct: 561 EALIISK 567
>NP_056190
          Length = 929

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           + LIIS+LN
Sbjct: 465 QELIISRLN 473
>NP_955749
          Length = 884

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           + LIIS+LN
Sbjct: 465 QELIISRLN 473
>NP_598328
          Length = 582

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 409 LVISKMN 415
>NP_003169
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 409 LVISKMN 415
>NP_443076
          Length = 536

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           EA++I +LN
Sbjct: 116 EAMVIRELN 124
>NP_008905
          Length = 810

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 154 LVISKIN 160
>NP_008886
          Length = 778

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 155 LVISKIN 161
>NP_001265108
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>NP_001265106
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 176 LVISKIN 182
>NP_001265105
          Length = 768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 112 LVISKIN 118
>NP_001265103
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>NP_001265102
          Length = 829

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 173 LVISKIN 179
>NP_001265100
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>NP_001265099
          Length = 817

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 161 LVISKIN 167
>NP_065904
          Length = 622

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   ALIISKLN 9
           AL++SKL+
Sbjct: 574 ALVVSKLS 581
>NP_056081
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           +A +ISKLN
Sbjct: 178 QADVISKLN 186
>NP_001247420
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           +A +ISKLN
Sbjct: 194 QADVISKLN 202
>NP_001265104
          Length = 733

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 77 LVISKIN 83
>NP_001265107
          Length = 699

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>NP_006409
          Length = 510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           ++ISKLN
Sbjct: 405 MVISKLN 411
>XP_006723819
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   EALIISKLN 9
           +A +ISKLN
Sbjct: 178 QADVISKLN 186
>XP_006718031
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>XP_006718030
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>XP_006718029
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>XP_005271882
          Length = 666

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LIISKLN 9
          L+ISK+N
Sbjct: 43 LVISKIN 49
>XP_006718028
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 168 LVISKIN 174
>XP_006718027
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 169 LVISKIN 175
>XP_006713376
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 152 LVISKMN 158
>XP_006713375
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 248 LVISKMN 254
>XP_006713374
          Length = 511

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 409 LVISKMN 415
>NP_008885
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LIISKLN 9
           L+ISK+N
Sbjct: 155 LVISKIN 161
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.134    0.307 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 246,240
Number of extensions: 232
Number of successful extensions: 38
Number of sequences better than 10000.0: 38
Number of HSP's gapped: 38
Number of HSP's successfully gapped: 38
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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