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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1135
XP_005262424                                                           18    1135
NP_001274685                                                           17    2528
NP_001274679                                                           17    2528
NP_001274678                                                           17    2528
NP_001274680                                                           17    2528
NP_001274672                                                           17    2528
NP_001274673                                                           17    2528
NP_001274674                                                           17    2528
NP_004453                                                              17    2528
NP_001274677                                                           17    2528
NP_001274671                                                           17    2528
NP_001274676                                                           17    2528
XP_006716310                                                           17    2528
NP_001278383                                                           16    5631
NP_055676                                                              16    5631
NP_001273456                                                           16    5631
XP_006721418                                                           16    5631
NP_001155059                                                           16    7355
NP_002094                                                              16    7355
NP_004361                                                              16    7355
XP_006715397                                                           16    7355
NP_055861                                                              15    9605
NP_001018865                                                           15    9605
NP_001018864                                                           15    9605
XP_005272230                                                           15    9605
XP_005272229                                                           15    9605
XP_005272228                                                           15    9605
XP_005247970                                                           15    9605
XP_005247968                                                           15    9605

>NP_722576
          Length = 3080

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DMTTLSQIT 9
           DMTT SQIT
Sbjct: 248 DMTTPSQIT 256



>XP_005262424
          Length = 2875

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  DMTTLSQIT 9
          DMTT SQIT
Sbjct: 43 DMTTPSQIT 51



>NP_001274685
          Length = 250

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 234 LTTLSQVT 241



>NP_001274679
          Length = 476

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 460 LTTLSQVT 467



>NP_001274678
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 337 LTTLSQVT 344



>NP_001274680
          Length = 332

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 316 LTTLSQVT 323



>NP_001274672
          Length = 417

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 401 LTTLSQVT 408



>NP_001274673
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 337 LTTLSQVT 344



>NP_001274674
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 337 LTTLSQVT 344



>NP_004453
          Length = 417

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 401 LTTLSQVT 408



>NP_001274677
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 337 LTTLSQVT 344



>NP_001274671
          Length = 417

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 401 LTTLSQVT 408



>NP_001274676
          Length = 353

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 337 LTTLSQVT 344



>XP_006716310
          Length = 358

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   MTTLSQIT 9
           +TTLSQ+T
Sbjct: 342 LTTLSQVT 349



>NP_001278383
          Length = 795

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  DMTTLSQI 8
          D+TTLS I
Sbjct: 87 DLTTLSNI 94



>NP_055676
          Length = 946

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DMTTLSQI 8
           D+TTLS I
Sbjct: 238 DLTTLSNI 245



>NP_001273456
          Length = 975

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DMTTLSQI 8
           D+TTLS I
Sbjct: 238 DLTTLSNI 245



>XP_006721418
          Length = 972

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   DMTTLSQI 8
           D+TTLS I
Sbjct: 238 DLTTLSNI 245



>NP_001155059
          Length = 673

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTLSQIT 9
           TT+SQIT
Sbjct: 192 TTVSQIT 198



>NP_002094
          Length = 737

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   TTLSQIT 9
           TT+SQIT
Sbjct: 256 TTVSQIT 262



>NP_004361
          Length = 3063

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DMTTLSQIT 9
            +MTTLS  T
Sbjct: 1173 EMTTLSDTT 1181



>XP_006715397
          Length = 2972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    DMTTLSQIT 9
            +MTTLS  T
Sbjct: 1082 EMTTLSDTT 1090



>NP_055861
          Length = 2677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DMTTLSQI 8
           DM T SQI
Sbjct: 348 DMVTCSQI 355



>NP_001018865
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  TTLSQIT 9
          TT+SQ+T
Sbjct: 78 TTISQLT 84



>NP_001018864
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  TTLSQIT 9
          TT+SQ+T
Sbjct: 78 TTISQLT 84



>XP_005272230
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DMTTLSQI 8
           DM T SQI
Sbjct: 348 DMVTCSQI 355



>XP_005272229
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DMTTLSQI 8
           DM T SQI
Sbjct: 348 DMVTCSQI 355



>XP_005272228
          Length = 2706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   DMTTLSQI 8
           DM T SQI
Sbjct: 348 DMVTCSQI 355



>XP_005247970
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  TTLSQIT 9
          TT+SQ+T
Sbjct: 78 TTISQLT 84



>XP_005247968
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  TTLSQIT 9
          TT+SQ+T
Sbjct: 78 TTISQLT 84



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.116    0.280 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 262,438
Number of extensions: 368
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)