Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001185761                                                           20     509
NP_001185763                                                           20     509
NP_055459                                                              20     509
XP_006726410                                                           20     509
XP_006711719                                                           20     509
XP_006711718                                                           20     509
XP_006711717                                                           20     509
XP_006711716                                                           20     509
XP_006711715                                                           20     509
XP_006711714                                                           20     509
XP_006711713                                                           20     509
XP_006711712                                                           20     509
XP_006711711                                                           20     509
XP_006711710                                                           20     509
XP_006711709                                                           20     509
XP_005277503                                                           20     509
XP_005273038                                                           20     509
XP_006711708                                                           20     509
XP_005277500                                                           20     509
XP_006711707                                                           20     509
XP_005277499                                                           20     509
XP_006711706                                                           20     509
XP_006711705                                                           20     509
XP_006711164                                                           20     509
XP_006710143                                                           20     509
XP_006710142                                                           20     509
XP_006710141                                                           20     509
NP_110443                                                              17    3301
XP_006710993                                                           17    3301
XP_005271292                                                           17    3301
XP_005271291                                                           17    3301
XP_006710992                                                           17    3301
NP_056211                                                              16    5631
NP_001269271                                                           16    5631
NP_001253970                                                           16    5631
NP_001253971                                                           16    5631
XP_006713630                                                           16    5631
XP_006713629                                                           16    5631
XP_006713628                                                           16    5631
XP_006713627                                                           16    5631
XP_005268526                                                           16    7355
NP_060176                                                              16    7355
NP_005248                                                              15    9605
NP_690042                                                              15    9605
NP_056456                                                              15    9605
NP_001230182                                                           15    9605
NP_001230579                                                           15    9605
NP_112215                                                              15    9605
NP_055797                                                              15    9605
NP_110446                                                              15    9605
NP_003877                                                              15    9605
XP_005258305                                                           15    9605
XP_005258287                                                           15    9605
XP_006722481                                                           15    9605
XP_005258286                                                           15    9605
XP_006722480                                                           15    9605
XP_005258284                                                           15    9605
XP_005267486                                                           15    9605
XP_006720145                                                           15    9605
XP_005267485                                                           15    9605
XP_005267484                                                           15    9605
XP_005267483                                                           15    9605
XP_005267482                                                           15    9605
XP_005267481                                                           15    9605
XP_005267480                                                           15    9605
XP_005267479                                                           15    9605
XP_005267478                                                           15    9605
XP_005267477                                                           15    9605
XP_005272028                                                           15    9605
XP_005272027                                                           15    9605
XP_005272026                                                           15    9605
XP_005272025                                                           15    9605
XP_005272024                                                           15    9605
NP_001034449                                                           15    9605
NP_001017372                                                           15    9605
NP_054750                                                              15    9605
>NP_001185761
          Length = 2240

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1471 DLLRSNDID 1479
>NP_001185763
          Length = 2362

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1515 DLLRSNDID 1523
>NP_055459
          Length = 2346

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1515 DLLRSNDID 1523
>XP_006726410
          Length = 2087

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1318 DLLRSNDID 1326
>XP_006711719
          Length = 2302

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1471 DLLRSNDID 1479
>XP_006711718
          Length = 1436

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLLMSNDID 9
           DLL SNDID
Sbjct: 522 DLLRSNDID 530
>XP_006711717
          Length = 2385

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1471 DLLRSNDID 1479
>XP_006711716
          Length = 2411

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1580 DLLRSNDID 1588
>XP_006711715
          Length = 2428

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1514 DLLRSNDID 1522
>XP_006711714
          Length = 2429

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1515 DLLRSNDID 1523
>XP_006711713
          Length = 2432

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1518 DLLRSNDID 1526
>XP_006711712
          Length = 2473

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_006711711
          Length = 2482

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1568 DLLRSNDID 1576
>XP_006711710
          Length = 2494

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1580 DLLRSNDID 1588
>XP_006711709
          Length = 2495

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1581 DLLRSNDID 1589
>XP_005277503
          Length = 2498

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_005273038
          Length = 2509

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_006711708
          Length = 2512

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_005277500
          Length = 2525

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_006711707
          Length = 2553

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_005277499
          Length = 2561

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_006711706
          Length = 2591

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1677 DLLRSNDID 1685
>XP_006711705
          Length = 2592

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1678 DLLRSNDID 1686
>XP_006711164
          Length = 2087

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    DLLMSNDID 9
            DLL SNDID
Sbjct: 1318 DLLRSNDID 1326
>XP_006710143
          Length = 562

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLLMSNDID 9
           DLL SNDID
Sbjct: 357 DLLRSNDID 365
>XP_006710142
          Length = 671

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLLMSNDID 9
           DLL SNDID
Sbjct: 466 DLLRSNDID 474
>XP_006710141
          Length = 672

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DLLMSNDID 9
           DLL SNDID
Sbjct: 467 DLLRSNDID 475
>NP_110443
          Length = 541

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DLLMSNDI 8
           D+LMS+DI
Sbjct: 318 DILMSSDI 325
>XP_006710993
          Length = 352

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DLLMSNDI 8
           D+LMS+DI
Sbjct: 318 DILMSSDI 325
>XP_005271292
          Length = 451

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DLLMSNDI 8
           D+LMS+DI
Sbjct: 318 DILMSSDI 325
>XP_005271291
          Length = 484

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DLLMSNDI 8
           D+LMS+DI
Sbjct: 318 DILMSSDI 325
>XP_006710992
          Length = 503

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   DLLMSNDI 8
           D+LMS+DI
Sbjct: 318 DILMSSDI 325
>NP_056211
          Length = 659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 577 DLIMTND 583
>NP_001269271
          Length = 600

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 518 DLIMTND 524
>NP_001253970
          Length = 642

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 560 DLIMTND 566
>NP_001253971
          Length = 606

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 524 DLIMTND 530
>XP_006713630
          Length = 620

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 591 DLIMTND 597
>XP_006713629
          Length = 631

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 549 DLIMTND 555
>XP_006713628
          Length = 659

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 577 DLIMTND 583
>XP_006713627
          Length = 673

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   DLLMSND 7
           DL+M+ND
Sbjct: 591 DLIMTND 597
>XP_005268526
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DLLMSNDID 9
          DLL + D+D
Sbjct: 51 DLLAAGDVD 59
>NP_060176
          Length = 383

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  DLLMSNDID 9
          DLL + D+D
Sbjct: 51 DLLAAGDVD 59
>NP_005248
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLLMSNDID 9
           DLL+  D+D
Sbjct: 111 DLLLFTDLD 119
>NP_690042
          Length = 412

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLLMSNDID 9
          D+L+SN+ D
Sbjct: 43 DVLLSNEED 51
>NP_056456
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  DLLMSNDID 9
          D+L+SN+ D
Sbjct: 52 DVLLSNEED 60
>NP_001230182
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLLMSNDID 9
           DL++S D D
Sbjct: 426 DLIISQDTD 434
>NP_001230579
          Length = 112

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  DLLMSNDID 9
          D+L+  D+D
Sbjct: 59 DILLHQDVD 67
>NP_112215
          Length = 521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLLMSNDID 9
           DL++S D D
Sbjct: 426 DLIISQDTD 434
>NP_055797
          Length = 2341

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 943 DLLEQQDID 951
>NP_110446
          Length = 385

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1  DLLMSNDID 9
          D+L+  D+D
Sbjct: 59 DILLHQDVD 67
>NP_003877
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LMSNDID 9
          +MS+DID
Sbjct: 9  MMSDDID 15
>XP_005258305
          Length = 595

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DLLMSNDID 9
           DLL+  D+D
Sbjct: 111 DLLLFTDLD 119
>XP_005258287
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>XP_006722481
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>XP_005258286
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>XP_006722480
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>XP_005258284
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>XP_005267486
          Length = 1421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  DLLMSNDID 9
          DLL   DID
Sbjct: 15 DLLEQQDID 23
>XP_006720145
          Length = 1423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  DLLMSNDID 9
          DLL   DID
Sbjct: 17 DLLEQQDID 25
>XP_005267485
          Length = 1459

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1  DLLMSNDID 9
          DLL   DID
Sbjct: 53 DLLEQQDID 61
>XP_005267484
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 943 DLLEQQDID 951
>XP_005267483
          Length = 2230

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 832 DLLEQQDID 840
>XP_005267482
          Length = 2243

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 837 DLLEQQDID 845
>XP_005267481
          Length = 2293

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 887 DLLEQQDID 895
>XP_005267480
          Length = 2336

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 938 DLLEQQDID 946
>XP_005267479
          Length = 2344

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 938 DLLEQQDID 946
>XP_005267478
          Length = 2348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 942 DLLEQQDID 950
>XP_005267477
          Length = 2349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   DLLMSNDID 9
           DLL   DID
Sbjct: 943 DLLEQQDID 951
>XP_005272028
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>XP_005272027
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>XP_005272026
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>XP_005272025
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>XP_005272024
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>NP_001034449
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 MSNDID 9
         M+NDID
Sbjct: 1 MANDID 6
>NP_001017372
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
>NP_054750
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   LLMSNDID 9
           LLMSN+ D
Sbjct: 110 LLMSNEPD 117
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.141    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 419,461
Number of extensions: 617
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)

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