Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_056262                                                              17    2528
NP_001269470                                                           17    2528
NP_003167                                                              17    2528
NP_001269471                                                           17    2528
XP_005263454                                                           17    2528
XP_005263453                                                           17    2528
XP_005271213                                                           17    2528
XP_005271212                                                           17    2528
XP_005271211                                                           17    2528
XP_006710922                                                           17    2528
NP_004027                                                              17    3301
XP_006711990                                                           17    3301
XP_005264162                                                           17    3301
XP_005264161                                                           17    3301
XP_005264159                                                           17    3301
XP_006711989                                                           17    3301
XP_006711988                                                           17    3301
NP_005276                                                              17    4312
NP_005597                                                              16    5631
NP_001008530                                                           16    5631
NP_001254711                                                           16    5631
NP_001254712                                                           16    5631
NP_060219                                                              16    5631
NP_001129631                                                           16    5631
NP_001129630                                                           16    5631
NP_001129629                                                           16    5631
NP_060072                                                              16    5631
XP_005278099                                                           16    5631
XP_005272679                                                           16    5631
XP_006724600                                                           16    5631
XP_005278096                                                           16    5631
XP_005267920                                                           16    5631
XP_005267919                                                           16    5631
XP_006718323                                                           16    5631
XP_005253071                                                           16    5631
XP_005253069                                                           16    5631
XP_005253068                                                           16    5631
XP_006718322                                                           16    5631
XP_005253066                                                           16    5631
NP_001139669                                                           16    7355
NP_000294                                                              15    9605
NP_758953                                                              15    9605
NP_002667                                                              15    9605
XP_005261699                                                           15    9605
XP_005261698                                                           15    9605
XP_005261694                                                           15    9605
XP_005255431                                                           15    9605
XP_005255430                                                           15    9605
XP_005255429                                                           15    9605
XP_005267412                                                           15    9605
XP_005267411                                                           15    9605
>NP_056262
          Length = 1602

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    KKRATFLL 9
            KKRA FLL
Sbjct: 1285 KKRAAFLL 1292
>NP_001269470
          Length = 976

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 793 DKKTQTFLL 801
>NP_003167
          Length = 976

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 793 DKKTQTFLL 801
>NP_001269471
          Length = 951

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 768 DKKTQTFLL 776
>XP_005263454
          Length = 1324

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    KKRATFLL 9
            KKRA FLL
Sbjct: 1007 KKRAAFLL 1014
>XP_005263453
          Length = 1613

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2    KKRATFLL 9
            KKRA FLL
Sbjct: 1296 KKRAAFLL 1303
>XP_005271213
          Length = 921

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 738 DKKTQTFLL 746
>XP_005271212
          Length = 958

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 775 DKKTQTFLL 783
>XP_005271211
          Length = 973

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 790 DKKTQTFLL 798
>XP_006710922
          Length = 976

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   DKKRATFLL 9
           DKK  TFLL
Sbjct: 793 DKKTQTFLL 801
>NP_004027
          Length = 1144

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 604 KKRNTFLL 611
>XP_006711990
          Length = 1131

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 626 KKRNTFLL 633
>XP_005264162
          Length = 1144

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 604 KKRNTFLL 611
>XP_005264161
          Length = 1145

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 604 KKRNTFLL 611
>XP_005264159
          Length = 1166

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 626 KKRNTFLL 633
>XP_006711989
          Length = 1167

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 626 KKRNTFLL 633
>XP_006711988
          Length = 1167

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFLL
Sbjct: 626 KKRNTFLL 633
>NP_005276
          Length = 328

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   KKRATFLL 9
           KKR TFL+
Sbjct: 246 KKRVTFLV 253
>NP_005597
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DKKRATFL 8
           D+KR+T+L
Sbjct: 222 DEKRSTYL 229
>NP_001008530
          Length = 433

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DKKRATFL 8
           D+KR+T+L
Sbjct: 222 DEKRSTYL 229
>NP_001254711
          Length = 1301

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>NP_001254712
          Length = 1179

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>NP_060219
          Length = 1208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>NP_001129631
          Length = 349

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 162 DKKRAT 167
>NP_001129630
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 379 DKKRAT 384
>NP_001129629
          Length = 566

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 379 DKKRAT 384
>NP_060072
          Length = 571

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 384 DKKRAT 389
>XP_005278099
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 167 DKKRAT 172
>XP_005272679
          Length = 567

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 379 DKKRAT 384
>XP_006724600
          Length = 571

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 384 DKKRAT 389
>XP_005278096
          Length = 572

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   DKKRAT 6
           DKKRAT
Sbjct: 384 DKKRAT 389
>XP_005267920
          Length = 372

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DKKRATFL 8
           D+KR+T+L
Sbjct: 222 DEKRSTYL 229
>XP_005267919
          Length = 376

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   DKKRATFL 8
           D+KR+T+L
Sbjct: 222 DEKRSTYL 229
>XP_006718323
          Length = 1119

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>XP_005253071
          Length = 1208

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>XP_005253069
          Length = 1238

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>XP_005253068
          Length = 1269

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>XP_006718322
          Length = 1298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>XP_005253066
          Length = 1298

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  DKKRATFLL 9
          D  R+TFLL
Sbjct: 51 DSPRSTFLL 59
>NP_001139669
          Length = 7081

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2    KKRATFLL 9
            KKR TF L
Sbjct: 1350 KKRVTFAL 1357
>NP_000294
          Length = 246

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KKRATFL 8
           KKR TF+
Sbjct: 114 KKRGTFI 120
>NP_758953
          Length = 422

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DKKRATFL 8
           +KKR TF+
Sbjct: 287 EKKRMTFV 294
>NP_002667
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KKRATFL 8
           KKR TF+
Sbjct: 123 KKRGTFI 129
>XP_005261699
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  KKRATFL 8
          KKR TF+
Sbjct: 8  KKRGTFI 14
>XP_005261698
          Length = 147

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  KKRATFL 8
          KKR TF+
Sbjct: 8  KKRGTFI 14
>XP_005261694
          Length = 288

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KKRATFL 8
           KKR TF+
Sbjct: 149 KKRGTFI 155
>XP_005255431
          Length = 163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  KKRATFL 8
          KKR TF+
Sbjct: 31 KKRGTFI 37
>XP_005255430
          Length = 187

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  KKRATFL 8
          KKR TF+
Sbjct: 31 KKRGTFI 37
>XP_005255429
          Length = 270

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   KKRATFL 8
           KKR TF+
Sbjct: 114 KKRGTFI 120
>XP_005267412
          Length = 378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DKKRATFL 8
           +KKR TF+
Sbjct: 243 EKKRMTFV 250
>XP_005267411
          Length = 410

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DKKRATFL 8
           +KKR TF+
Sbjct: 275 EKKRMTFV 282
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.142    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 456,015
Number of extensions: 687
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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