Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001167558                                                           18    1135
NP_858045                                                              18    1135
NP_006525                                                              18    1135
NP_001167559                                                           18    1135
NP_005302                                                              16    5631
NP_001001549                                                           16    5631
XP_006715767                                                           16    5631
XP_006715766                                                           16    5631
XP_005271818                                                           16    5631
XP_005271817                                                           16    5631
NP_001171989                                                           16    7355
NP_001171991                                                           16    7355
NP_001171990                                                           16    7355
NP_932766                                                              16    7355
NP_689939                                                              16    7355
>NP_001167558
          Length = 1423

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DINAS EMR
Sbjct: 210 DINASPEMR 218
>NP_858045
          Length = 1424

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DINAS EMR
Sbjct: 210 DINASPEMR 218
>NP_006525
          Length = 1420

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DINAS EMR
Sbjct: 210 DINASPEMR 218
>NP_001167559
          Length = 1415

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DINAS EMR
Sbjct: 210 DINASPEMR 218
>NP_005302
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 58 DMNASLE 64
>NP_001001549
          Length = 548

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 58 DMNASLE 64
>XP_006715767
          Length = 588

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 52 DMNASLE 58
>XP_006715766
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 58 DMNASLE 64
>XP_005271818
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 58 DMNASLE 64
>XP_005271817
          Length = 594

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  DINASLE 7
          D+NASLE
Sbjct: 58 DMNASLE 64
>NP_001171989
          Length = 186

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DIN+ +EM+
Sbjct: 176 DINSRVEMK 184
>NP_001171991
          Length = 154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DIN+ +EM+
Sbjct: 144 DINSRVEMK 152
>NP_001171990
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DIN+ +EM+
Sbjct: 208 DINSRVEMK 216
>NP_932766
          Length = 269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           DIN+ +EM+
Sbjct: 259 DINSRVEMK 267
>NP_689939
          Length = 434

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   DINASLEMR 9
           +IN +L+MR
Sbjct: 229 EINTTLQMR 237
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.127    0.313 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,506
Number of extensions: 377
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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