Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     665
NP_000240                                                              19     665
NP_001161089                                                           19     665
XP_005265218                                                           19     665
NP_002079                                                              17    3301
XP_006723233                                                           17    3301
XP_006723232                                                           17    3301
XP_005258879                                                           17    3301
XP_005258878                                                           17    3301
NP_001123608                                                           17    4312
NP_065902                                                              17    4312
NP_958934                                                              17    4312
NP_001123610                                                           17    4312
NP_001123609                                                           17    4312
NP_005536                                                              17    4312
NP_078966                                                              16    5631
NP_061910                                                              16    5631
NP_004915                                                              16    7355
NP_001129494                                                           16    7355
NP_001185896                                                           16    7355
XP_005259339                                                           16    7355
XP_005259338                                                           16    7355
XP_006723469                                                           16    7355
NP_001245210                                                           15    9605
NP_000242                                                              15    9605
NP_003208                                                              15    9605
NP_001092046                                                           15    9605
NP_001277403                                                           15    9605
NP_055622                                                              15    9605
NP_004845                                                              15    9605
NP_073745                                                              15    9605
NP_001252541                                                           15    9605
NP_057368                                                              15    9605
NP_996882                                                              15    9605
XP_005269183                                                           15    9605
XP_005271799                                                           15    9605
XP_005271798                                                           15    9605
XP_005271797                                                           15    9605
XP_005271796                                                           15    9605
XP_006719011                                                           15    9605
XP_005264389                                                           15    9605
NP_001073957                                                           15    9605
>NP_001245200
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           +ALASISHV
Sbjct: 102 EALASISHV 110
>NP_000240
          Length = 756

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           +ALASISHV
Sbjct: 102 EALASISHV 110
>NP_001161089
          Length = 658

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  DALASISHV 9
          +ALASISHV
Sbjct: 4  EALASISHV 12
>XP_005265218
          Length = 687

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           +ALASISHV
Sbjct: 102 EALASISHV 110
>NP_002079
          Length = 980

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DALASISHV 9
           DA ASISH+
Sbjct: 217 DANASISHL 225
>XP_006723233
          Length = 912

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DALASISHV 9
           DA ASISH+
Sbjct: 149 DANASISHL 157
>XP_006723232
          Length = 981

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DALASISHV 9
           DA ASISH+
Sbjct: 217 DANASISHL 225
>XP_005258879
          Length = 942

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DALASISHV 9
           DA ASISH+
Sbjct: 217 DANASISHL 225
>XP_005258878
          Length = 980

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   DALASISHV 9
           DA ASISH+
Sbjct: 217 DANASISHL 225
>NP_001123608
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           DAL+++SH+
Sbjct: 168 DALSALSHL 176
>NP_065902
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           DAL+++SH+
Sbjct: 168 DALSALSHL 176
>NP_958934
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALASISHV 9
           ALAS+SH+
Sbjct: 93  ALASLSHL 100
>NP_001123610
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           DAL+++SH+
Sbjct: 168 DALSALSHL 176
>NP_001123609
          Length = 745

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   DALASISHV 9
           DAL+++SH+
Sbjct: 168 DALSALSHL 176
>NP_005536
          Length = 428

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2   ALASISHV 9
           ALAS+SH+
Sbjct: 93  ALASLSHL 100
>NP_078966
          Length = 14507

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1    DALASIS 7
            DALASIS
Sbjct: 4576 DALASIS 4582
>NP_061910
          Length = 245

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   ALASISHV 9
           ALA +SHV
Sbjct: 169 ALADLSHV 176
>NP_004915
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DALASISH 8
           DAL S++H
Sbjct: 503 DALGSLTH 510
>NP_001129494
          Length = 964

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          +LASISH
Sbjct: 12 SLASISH 18
>NP_001185896
          Length = 857

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  ALASISH 8
          +LASISH
Sbjct: 12 SLASISH 18
>XP_005259339
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DALASISH 8
           DAL S++H
Sbjct: 503 DALGSLTH 510
>XP_005259338
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DALASISH 8
           DAL S++H
Sbjct: 503 DALGSLTH 510
>XP_006723469
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DALASISH 8
           DAL S++H
Sbjct: 503 DALGSLTH 510
>NP_001245210
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DALASISHV 9
           DA+ S +HV
Sbjct: 537 DAVVSFAHV 545
>NP_000242
          Length = 934

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DALASISHV 9
           DA+ S +HV
Sbjct: 603 DAVVSFAHV 611
>NP_003208
          Length = 237

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DALASISHV 9
          DAL   SH+
Sbjct: 11 DALLKFSHI 19
>NP_001092046
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DALASISHV 9
          DAL   SH+
Sbjct: 69 DALLKFSHI 77
>NP_001277403
          Length = 706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>NP_055622
          Length = 707

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>NP_004845
          Length = 356

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   DALASISH 8
           DAL ++SH
Sbjct: 95  DALKNLSH 102
>NP_073745
          Length = 445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  DALASISH 8
          D+ AS+SH
Sbjct: 40 DSSASVSH 47
>NP_001252541
          Length = 2371

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 970 LASISH 975
>NP_057368
          Length = 2376

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 975 LASISH 980
>NP_996882
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LASISH 8
           LASISH
Sbjct: 975 LASISH 980
>XP_005269183
          Length = 295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  DALASISHV 9
          DAL   SH+
Sbjct: 69 DALLKFSHI 77
>XP_005271799
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>XP_005271798
          Length = 474

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>XP_005271797
          Length = 643

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>XP_005271796
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>XP_006719011
          Length = 670

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ASISHV 9
           ASISHV
Sbjct: 135 ASISHV 140
>XP_005264389
          Length = 921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   DALASISHV 9
           DA+ S +HV
Sbjct: 603 DAVVSFAHV 611
>NP_001073957
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   ALASISHV 9
           ALAS+ H+
Sbjct: 177 ALASVGHL 184
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.316    0.122    0.311 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 407,912
Number of extensions: 581
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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