Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              20     390
NP_066124                                                              20     390
XP_006717999                                                           20     390
NP_006747                                                              17    2528
NP_958845                                                              17    2528
XP_006716530                                                           17    2528
NP_653240                                                              17    3301
NP_001159446                                                           17    3301
NP_001252506                                                           17    4312
NP_001251503                                                           17    4312
XP_005257134                                                           17    4312
XP_005255536                                                           17    4312
XP_005255535                                                           17    4312
XP_005255532                                                           17    4312
NP_775113                                                              15    9605
NP_077320                                                              15    9605
NP_775111                                                              15    9605
NP_775110                                                              15    9605
NP_775112                                                              15    9605
NP_000061                                                              15    9605
NP_112494                                                              15    9605
NP_065729                                                              15    9605
XP_005265394                                                           15    9605
>NP_065681
          Length = 1072

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   CRTMIAAAV 9
           CRT+IAAAV
Sbjct: 634 CRTVIAAAV 642
>NP_066124
          Length = 1114

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   CRTMIAAAV 9
           CRT+IAAAV
Sbjct: 634 CRTVIAAAV 642
>XP_006717999
          Length = 1207

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   CRTMIAAAV 9
           CRT+IAAAV
Sbjct: 727 CRTVIAAAV 735
>NP_006747
          Length = 301

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CR M+AAA+
Sbjct: 144 CREMLAAAL 152
>NP_958845
          Length = 280

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CR M+AAA+
Sbjct: 123 CREMLAAAL 131
>XP_006716530
          Length = 254

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CR M+AAA+
Sbjct: 97  CREMLAAAL 105
>NP_653240
          Length = 676

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           C+T +AAA+
Sbjct: 95  CQTKVAAAI 103
>NP_001159446
          Length = 736

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           C+T +AAA+
Sbjct: 95  CQTKVAAAI 103
>NP_001252506
          Length = 852

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CRT++ AA+
Sbjct: 320 CRTVMIAAI 328
>NP_001251503
          Length = 833

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CRT++ AA+
Sbjct: 320 CRTVMIAAI 328
>XP_005257134
          Length = 842

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           CRT++ AA+
Sbjct: 329 CRTVMIAAI 337
>XP_005255536
          Length = 332

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  RTMIAAAV 9
          R+MIAAAV
Sbjct: 54 RSMIAAAV 61
>XP_005255535
          Length = 332

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2  RTMIAAAV 9
          R+MIAAAV
Sbjct: 54 RSMIAAAV 61
>XP_005255532
          Length = 403

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   RTMIAAAV 9
           R+MIAAAV
Sbjct: 125 RSMIAAAV 132
>NP_775113
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CRTMIA 6
          CR+MIA
Sbjct: 32 CRSMIA 37
>NP_077320
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CRTMIA 6
           CR+MIA
Sbjct: 691 CRSMIA 696
>NP_775111
          Length = 309

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CRTMIA 6
           CR+MIA
Sbjct: 185 CRSMIA 190
>NP_775110
          Length = 729

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CRTMIA 6
           CR+MIA
Sbjct: 605 CRSMIA 610
>NP_775112
          Length = 156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CRTMIA 6
          CR+MIA
Sbjct: 32 CRSMIA 37
>NP_000061
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   CRTMIA 6
           CR+MIA
Sbjct: 697 CRSMIA 702
>NP_112494
          Length = 898

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CRTMIAAAV 9
           C+T++ AAV
Sbjct: 324 CQTIMIAAV 332
>NP_065729
          Length = 345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRTMIAAAV 9
           C  M+AAA+
Sbjct: 300 CLMMLAAAI 308
>XP_005265394
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CRTMIAAAV 9
           C  M+AAA+
Sbjct: 224 CLMMLAAAI 232
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.344    0.128    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 292,816
Number of extensions: 275
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)

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