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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     390
NP_009297                                                              20     390
NP_659491                                                              20     509
NP_851322                                                              17    4312
NP_001026                                                              16    5631
NP_001693                                                              16    5631
XP_005259184                                                           16    5631
XP_005251072                                                           16    5631
XP_006711873                                                           16    5631
XP_006711872                                                           16    5631
XP_006711871                                                           16    5631
XP_006711870                                                           16    5631
XP_006711869                                                           16    5631
XP_006711868                                                           16    5631
XP_006711867                                                           16    5631
XP_006711866                                                           16    5631
XP_006711865                                                           16    5631
NP_009049                                                              16    7355
NP_001139                                                              16    7355
NP_543007                                                              16    7355
NP_848652                                                              16    7355
XP_006714558                                                           16    7355
XP_006714257                                                           16    7355
XP_005263002                                                           16    7355
XP_005262999                                                           16    7355
XP_006714252                                                           16    7355
XP_006714251                                                           16    7355
XP_006714250                                                           16    7355
XP_006710219                                                           16    7355
NP_062545                                                              16    7355
NP_060128                                                              15    9605
XP_006720237                                                           15    9605
XP_005267827                                                           15    9605
XP_005267826                                                           15    9605

>NP_005148
          Length = 1130

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    CLAISRSSE 9
            CLAISR+SE
Sbjct: 1040 CLAISRNSE 1048



>NP_009297
          Length = 1149

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    CLAISRSSE 9
            CLAISR+SE
Sbjct: 1059 CLAISRNSE 1067



>NP_659491
          Length = 620

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  CLAISRSSE 9
          CLAISRS E
Sbjct: 69 CLAISRSGE 77



>NP_851322
          Length = 724

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   CLAISRSSE 9
           CL +S SSE
Sbjct: 386 CLVLSTSSE 394



>NP_001026
          Length = 4967

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>NP_001693
          Length = 1584

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CLA SRSS
Sbjct: 196 CLAGSRSS 203



>XP_005259184
          Length = 576

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  LAISRSSE 9
          LA+SRS+E
Sbjct: 45 LAVSRSTE 52



>XP_005251072
          Length = 1586

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CLA SRSS
Sbjct: 196 CLAGSRSS 203



>XP_006711873
          Length = 4954

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711872
          Length = 2735

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711871
          Length = 4906

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711870
          Length = 4965

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711869
          Length = 4973

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711868
          Length = 4975

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711867
          Length = 4977

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711866
          Length = 4984

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>XP_006711865
          Length = 4985

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CL+ SRSS
Sbjct: 132 CLSTSRSS 139



>NP_009049
          Length = 3097

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    CLAISRSS 8
            C+A SRSS
Sbjct: 1717 CIAHSRSS 1724



>NP_001139
          Length = 3957

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1669 LAVGRSSE 1676



>NP_543007
          Length = 336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CLAISRSS 8
           CLA +RSS
Sbjct: 296 CLASARSS 303



>NP_848652
          Length = 302

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CLAISRS 7
           C A+SRS
Sbjct: 128 CFAVSRS 134



>XP_006714558
          Length = 2612

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    CLAISRSS 8
            C+A SRSS
Sbjct: 1232 CIAHSRSS 1239



>XP_006714257
          Length = 4145

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1678 LAVGRSSE 1685



>XP_005263002
          Length = 4072

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1636 LAVGRSSE 1643



>XP_005262999
          Length = 4147

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1680 LAVGRSSE 1687



>XP_006714252
          Length = 4153

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1686 LAVGRSSE 1693



>XP_006714251
          Length = 4165

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1698 LAVGRSSE 1705



>XP_006714250
          Length = 4190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    LAISRSSE 9
            LA+ RSSE
Sbjct: 1723 LAVGRSSE 1730



>XP_006710219
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  CLAISRS 7
          C A+SRS
Sbjct: 44 CFAVSRS 50



>NP_062545
          Length = 299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1  CLAISR 6
          CLA+SR
Sbjct: 50 CLAVSR 55



>NP_060128
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CLAISRSSE 9
           C+ ISR +E
Sbjct: 137 CIGISRFAE 145



>XP_006720237
          Length = 332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CLAISRSSE 9
           C+ ISR +E
Sbjct: 151 CIGISRFAE 159



>XP_005267827
          Length = 571

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CLAISRSSE 9
           C+ ISR +E
Sbjct: 137 CIGISRFAE 145



>XP_005267826
          Length = 585

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CLAISRSSE 9
           C+ ISR +E
Sbjct: 151 CIGISRFAE 159



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.119    0.320 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 330,623
Number of extensions: 416
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)