Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              18    1135
NP_671714                                                              18    1135
XP_006715890                                                           18    1135
XP_005250160                                                           18    1135
XP_005250159                                                           18    1135
NP_009041                                                              17    2528
NP_060289                                                              17    2528
NP_001158058                                                           17    2528
XP_005268084                                                           17    2528
XP_006711681                                                           17    2528
XP_006711680                                                           17    2528
XP_005245637                                                           17    2528
NP_689914                                                              17    3301
XP_005248296                                                           17    3301
XP_005248295                                                           17    3301
NP_009208                                                              17    3301
NP_056144                                                              17    4312
XP_006722774                                                           17    4312
XP_005259895                                                           17    4312
XP_005259894                                                           17    4312
NP_000129                                                              16    5631
NP_060440                                                              16    5631
XP_006720249                                                           16    5631
NP_060444                                                              16    7355
NP_110430                                                              16    7355
NP_001177195                                                           16    7355
NP_001177196                                                           16    7355
NP_005692                                                              16    7355
NP_001036053                                                           16    7355
NP_001036046                                                           16    7355
XP_005246139                                                           16    7355
NP_001990                                                              15    9605
NP_001269419                                                           15    9605
NP_001269423                                                           15    9605
NP_001269397                                                           15    9605
NP_001269422                                                           15    9605
NP_001269413                                                           15    9605
NP_001269401                                                           15    9605
NP_001264237                                                           15    9605
NP_005038                                                              15    9605
NP_001034589                                                           15    9605
NP_064522                                                              15    9605
NP_001257356                                                           15    9605
XP_006725625                                                           15    9605
XP_006725624                                                           15    9605
XP_006725615                                                           15    9605
XP_006725614                                                           15    9605
XP_006725613                                                           15    9605
XP_006725612                                                           15    9605
XP_006725611                                                           15    9605
XP_005272685                                                           15    9605
XP_006720735                                                           15    9605
XP_006720734                                                           15    9605
XP_006720733                                                           15    9605
XP_006720718                                                           15    9605
XP_006720717                                                           15    9605
XP_005268342                                                           15    9605
XP_005268341                                                           15    9605
XP_006720714                                                           15    9605
XP_006720696                                                           15    9605
XP_005270009                                                           15    9605
XP_006710423                                                           15    9605
NP_612144                                                              15    9605
>NP_005742
          Length = 3907

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    INTISSLK 9
            INTISSLK
Sbjct: 2988 INTISSLK 2995
>NP_671714
          Length = 3899

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    INTISSLK 9
            INTISSLK
Sbjct: 2980 INTISSLK 2987
>XP_006715890
          Length = 3860

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    INTISSLK 9
            INTISSLK
Sbjct: 2941 INTISSLK 2948
>XP_005250160
          Length = 3939

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    INTISSLK 9
            INTISSLK
Sbjct: 3020 INTISSLK 3027
>XP_005250159
          Length = 3952

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    INTISSLK 9
            INTISSLK
Sbjct: 3033 INTISSLK 3040
>NP_009041
          Length = 2627

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   CINTISSLK 9
           C+ T+SSLK
Sbjct: 111 CLATLSSLK 119
>NP_060289
          Length = 403

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  CINTISSLK 9
          C++TISS K
Sbjct: 78 CVSTISSSK 86
>NP_001158058
          Length = 3708

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   INTISSLK 9
           INTISSL+
Sbjct: 213 INTISSLR 220
>XP_005268084
          Length = 2627

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   CINTISSLK 9
           C+ T+SSLK
Sbjct: 111 CLATLSSLK 119
>XP_006711681
          Length = 3448

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   INTISSLK 9
           INTISSL+
Sbjct: 213 INTISSLR 220
>XP_006711680
          Length = 3617

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   INTISSLK 9
           INTISSL+
Sbjct: 213 INTISSLR 220
>XP_005245637
          Length = 3683

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   INTISSLK 9
           INTISSL+
Sbjct: 213 INTISSLR 220
>NP_689914
          Length = 5058

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3    NTISSLK 9
            NTISSLK
Sbjct: 1386 NTISSLK 1392
>XP_005248296
          Length = 630

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  INTISSLK 9
          INTI SLK
Sbjct: 90 INTIESLK 97
>XP_005248295
          Length = 745

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  INTISSLK 9
          INTI SLK
Sbjct: 90 INTIESLK 97
>NP_009208
          Length = 745

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2  INTISSLK 9
          INTI SLK
Sbjct: 90 INTIESLK 97
>NP_056144
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           CI TIS+L
Sbjct: 259 CIQTISTL 266
>XP_006722774
          Length = 531

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           CI TIS+L
Sbjct: 298 CIQTISTL 305
>XP_005259895
          Length = 624

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           CI TIS+L
Sbjct: 270 CIQTISTL 277
>XP_005259894
          Length = 651

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           CI TIS+L
Sbjct: 298 CIQTISTL 305
>NP_000129
          Length = 2871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   CINTISS 7
           C NT+SS
Sbjct: 304 CTNTVSS 310
>NP_060440
          Length = 1164

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   CINTISSLK 9
           C++T+S++K
Sbjct: 444 CLSTLSAVK 452
>XP_006720249
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   CINTISSLK 9
           C++T+S++K
Sbjct: 444 CLSTLSAVK 452
>NP_060444
          Length = 588

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CINTISSLK 9
           C+ TIS L+
Sbjct: 134 CLQTISDLE 142
>NP_110430
          Length = 607

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CINTISSLK 9
           C+ TIS L+
Sbjct: 134 CLQTISDLE 142
>NP_001177195
          Length = 523

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CINTISSLK 9
          C+ TIS L+
Sbjct: 50 CLQTISDLE 58
>NP_001177196
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  CINTISSLK 9
          C+ TIS L+
Sbjct: 18 CLQTISDLE 26
>NP_005692
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CINTISSL 8
           C+N  SSL
Sbjct: 148 CVNRFSSL 155
>NP_001036053
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CINTISSL 8
           C+N  SSL
Sbjct: 148 CVNRFSSL 155
>NP_001036046
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   CINTISSL 8
           C+N  SSL
Sbjct: 148 CVNRFSSL 155
>XP_005246139
          Length = 624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CINTISSLK 9
           C+ TIS L+
Sbjct: 134 CLQTISDLE 142
>NP_001990
          Length = 2912

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   CINTISSLK 9
           CI T+SS +
Sbjct: 510 CIPTVSSYR 518
>NP_001269419
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001269423
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001269397
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001269422
          Length = 630

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001269413
          Length = 631

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001269401
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_001264237
          Length = 632

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>NP_005038
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           C++ ISSL
Sbjct: 361 CLDAISSL 368
>NP_001034589
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTISSLK 9
           NT+SS+K
Sbjct: 993 NTLSSMK 999
>NP_064522
          Length = 841

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   INTISSLK 9
           + TISSLK
Sbjct: 583 LKTISSLK 590
>NP_001257356
          Length = 461

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   CINTISSL 8
           C++ ISSL
Sbjct: 318 CLDAISSL 325
>XP_006725625
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 85 NTIKSLK 91
>XP_006725624
          Length = 628

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 117 NTIKSLK 123
>XP_006725615
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 83 NTIKSLK 89
>XP_006725614
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 85 NTIKSLK 91
>XP_006725613
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 144 NTIKSLK 150
>XP_006725612
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 144 NTIKSLK 150
>XP_006725611
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>XP_005272685
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   INTISSLK 9
           + TISSLK
Sbjct: 557 LKTISSLK 564
>XP_006720735
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 85 NTIKSLK 91
>XP_006720734
          Length = 594

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 83 NTIKSLK 89
>XP_006720733
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 83 NTIKSLK 89
>XP_006720718
          Length = 617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 83 NTIKSLK 89
>XP_006720717
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 85 NTIKSLK 91
>XP_005268342
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 144 NTIKSLK 150
>XP_005268341
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 144 NTIKSLK 150
>XP_006720714
          Length = 619

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3  NTISSLK 9
          NTI SLK
Sbjct: 85 NTIKSLK 91
>XP_006720696
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   NTISSLK 9
           NTI SLK
Sbjct: 98  NTIKSLK 104
>XP_005270009
          Length = 640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   INTISSL 8
           +NT+SSL
Sbjct: 251 LNTVSSL 257
>XP_006710423
          Length = 932

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   NTISSLK 9
           NT+SS+K
Sbjct: 710 NTLSSMK 716
>NP_612144
          Length = 740

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   CINTISSLK 9
           C++TIS+ K
Sbjct: 564 CMDTISNKK 572
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.128    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 367,194
Number of extensions: 656
Number of successful extensions: 63
Number of sequences better than 10000.0: 63
Number of HSP's gapped: 63
Number of HSP's successfully gapped: 63
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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