Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              19     869
NP_066124                                                              19     869
XP_006717999                                                           19     869
NP_003256                                                              17    4312
NP_001258523                                                           17    4312
NP_001186862                                                           17    4312
NP_055269                                                              17    4312
NP_653266                                                              17    4312
NP_001186863                                                           17    4312
XP_006714357                                                           17    4312
NP_001158238                                                           16    7355
NP_207646                                                              16    7355
NP_078997                                                              16    7355
NP_008816                                                              16    7355
XP_005256014                                                           16    7355
XP_006720523                                                           16    7355
XP_006720345                                                           16    7355
XP_005268192                                                           16    7355
XP_005268191                                                           16    7355
NP_005735                                                              16    7355
NP_055672                                                              15    9605
NP_001273670                                                           15    9605
NP_116296                                                              15    9605
NP_001273668                                                           15    9605
NP_001273671                                                           15    9605
NP_001273669                                                           15    9605
NP_957717                                                              15    9605
NP_036329                                                              15    9605
NP_065756                                                              15    9605
NP_001186036                                                           15    9605
NP_001122062                                                           15    9605
NP_000491                                                              15    9605
NP_001273283                                                           15    9605
NP_683766                                                              15    9605
NP_001273284                                                           15    9605
NP_001182209                                                           15    9605
NP_003239                                                              15    9605
XP_006726140                                                           15    9605
XP_006726139                                                           15    9605
XP_006725960                                                           15    9605
XP_006725959                                                           15    9605
XP_006725536                                                           15    9605
XP_006725535                                                           15    9605
XP_005255506                                                           15    9605
XP_006720970                                                           15    9605
XP_006717095                                                           15    9605
XP_006717094                                                           15    9605
XP_005251923                                                           15    9605
XP_006715065                                                           15    9605
XP_006715064                                                           15    9605
XP_006711756                                                           15    9605
XP_005245738                                                           15    9605
XP_006711755                                                           15    9605
XP_005245737                                                           15    9605
>NP_065681
          Length = 1072

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           A VLLLHFN
Sbjct: 386 AGVLLLHFN 394
>NP_066124
          Length = 1114

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           A VLLLHFN
Sbjct: 386 AGVLLLHFN 394
>XP_006717999
          Length = 1207

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           A VLLLHFN
Sbjct: 479 AGVLLLHFN 487
>NP_003256
          Length = 904

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VVLLLHF 8
           +VLL+HF
Sbjct: 719 IVLLIHF 725
>NP_001258523
          Length = 353

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AVVLLLHFN 9
          AVVLL H++
Sbjct: 54 AVVLLAHYH 62
>NP_001186862
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           AVVLL H++
Sbjct: 202 AVVLLTHYH 210
>NP_055269
          Length = 551

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           AVVLL H++
Sbjct: 261 AVVLLTHYH 269
>NP_653266
          Length = 492

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           AVVLL H++
Sbjct: 193 AVVLLAHYH 201
>NP_001186863
          Length = 426

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AVVLLLHFN 9
           AVVLL H++
Sbjct: 136 AVVLLTHYH 144
>XP_006714357
          Length = 904

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   VVLLLHF 8
           +VLL+HF
Sbjct: 719 IVLLIHF 725
>NP_001158238
          Length = 2789

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VLLLHFN 9
           +LL+H+N
Sbjct: 645 ILLVHYN 651
>NP_207646
          Length = 2572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    VLLLHFN 9
            +LL+H+N
Sbjct: 1205 ILLVHYN 1211
>NP_078997
          Length = 3616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    VLLLHFN 9
            +LL+H+N
Sbjct: 1555 ILLVHYN 1561
>NP_008816
          Length = 3703

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    VLLLHFN 9
            +LL+H+N
Sbjct: 1559 ILLVHYN 1565
>XP_005256014
          Length = 3702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    VLLLHFN 9
            +LL+H+N
Sbjct: 1558 ILLVHYN 1564
>XP_006720523
          Length = 519

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLLHFN 9
           +LL HFN
Sbjct: 133 ILLSHFN 139
>XP_006720345
          Length = 1522

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VLLLHFN 9
           +LL+H+N
Sbjct: 155 ILLVHYN 161
>XP_005268192
          Length = 1602

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   VLLLHFN 9
           +LL+H+N
Sbjct: 235 ILLVHYN 241
>XP_005268191
          Length = 2572

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3    VLLLHFN 9
            +LL+H+N
Sbjct: 1205 ILLVHYN 1211
>NP_005735
          Length = 557

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLLHFN 9
           +LL HFN
Sbjct: 133 ILLSHFN 139
>NP_055672
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 627 AAVLLLH 633
>NP_001273670
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 228 AIVLVLH 234
>NP_116296
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 188 AIVLVLH 194
>NP_001273668
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 188 AIVLVLH 194
>NP_001273671
          Length = 420

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 228 AIVLVLH 234
>NP_001273669
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 188 AIVLVLH 194
>NP_957717
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   AVVLLLH 7
           A+VL+LH
Sbjct: 188 AIVLVLH 194
>NP_036329
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 655 AAVLLLH 661
>NP_065756
          Length = 553

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VVLLLHFN 9
           V LLLH++
Sbjct: 138 VALLLHYS 145
>NP_001186036
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VVLLLHFN 9
           V LLLH++
Sbjct: 138 VALLLHYS 145
>NP_001122062
          Length = 465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 274 AVVFLLH 280
>NP_000491
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 304 AVVFLLH 310
>NP_001273283
          Length = 751

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VVLLLHF 8
           V+LL HF
Sbjct: 460 VILLCHF 466
>NP_683766
          Length = 926

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VVLLLHF 8
           V+LL HF
Sbjct: 635 VILLCHF 641
>NP_001273284
          Length = 855

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VVLLLHF 8
           V+LL HF
Sbjct: 564 VILLCHF 570
>NP_001182209
          Length = 287

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   VLLLHFN 9
           +LLLH N
Sbjct: 115 ILLLHHN 121
>NP_003239
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  AVVLLLH 7
          A VLLLH
Sbjct: 7  AAVLLLH 13
>XP_006726140
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 106 AVVFLLH 112
>XP_006726139
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 263 AVVFLLH 269
>XP_006725960
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 106 AVVFLLH 112
>XP_006725959
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 263 AVVFLLH 269
>XP_006725536
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 106 AVVFLLH 112
>XP_006725535
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 263 AVVFLLH 269
>XP_005255506
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VVLLLHFN 9
           V LLLH++
Sbjct: 138 VALLLHYS 145
>XP_006720970
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VVLLLHFN 9
           V LLLH++
Sbjct: 138 VALLLHYS 145
>XP_006717095
          Length = 900

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 486 AAVLLLH 492
>XP_006717094
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 655 AAVLLLH 661
>XP_005251923
          Length = 1069

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 655 AAVLLLH 661
>XP_006715065
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 106 AVVFLLH 112
>XP_006715064
          Length = 454

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           AVV LLH
Sbjct: 263 AVVFLLH 269
>XP_006711756
          Length = 985

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 627 AAVLLLH 633
>XP_005245738
          Length = 1014

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 590 AAVLLLH 596
>XP_006711755
          Length = 1051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 627 AAVLLLH 633
>XP_005245737
          Length = 1051

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   AVVLLLH 7
           A VLLLH
Sbjct: 627 AAVLLLH 633
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.346    0.153    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 507,269
Number of extensions: 741
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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