Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              20     390
NP_009297                                                              20     390
NP_689777                                                              18    1482
NP_060360                                                              18    1482
XP_005250858                                                           18    1482
XP_005250857                                                           18    1482
NP_997046                                                              17    4312
NP_951038                                                              16    5631
NP_001159817                                                           16    5631
NP_001127812                                                           16    5631
NP_115678                                                              16    5631
XP_006715855                                                           16    5631
NP_001070671                                                           16    7355
NP_001269732                                                           16    7355
NP_001269731                                                           16    7355
NP_001269730                                                           16    7355
NP_065831                                                              16    7355
NP_065705                                                              16    7355
NP_065825                                                              16    7355
XP_005247719                                                           16    7355
NP_065391                                                              15    9605
NP_976328                                                              15    9605
NP_055999                                                              15    9605
NP_001137854                                                           15    9605
NP_061874                                                              15    9605
NP_001269786                                                           15    9605
NP_065868                                                              15    9605
XP_005261680                                                           15    9605
XP_005247738                                                           15    9605
XP_005247737                                                           15    9605
XP_006713778                                                           15    9605
XP_005247601                                                           15    9605
XP_006713735                                                           15    9605
XP_006713734                                                           15    9605
XP_006713136                                                           15    9605
>NP_005148
          Length = 1130

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVNSDVAKP 9
           AVNSD AKP
Sbjct: 940 AVNSDAAKP 948
>NP_009297
          Length = 1149

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVNSDVAKP 9
           AVNSD AKP
Sbjct: 959 AVNSDAAKP 967
>NP_689777
          Length = 3997

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    VNSDVAKP 9
            V+SDVAKP
Sbjct: 1855 VDSDVAKP 1862
>NP_060360
          Length = 4022

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    VNSDVAKP 9
            V+SDVAKP
Sbjct: 1880 VDSDVAKP 1887
>XP_005250858
          Length = 4022

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    VNSDVAKP 9
            V+SDVAKP
Sbjct: 1880 VDSDVAKP 1887
>XP_005250857
          Length = 4022

 Score = 18.1 bits (35), Expect =  1482
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    VNSDVAKP 9
            V+SDVAKP
Sbjct: 1880 VDSDVAKP 1887
>NP_997046
          Length = 547

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   AVNSDVAKP 9
           +VNSD +KP
Sbjct: 155 SVNSDNSKP 163
>NP_951038
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AVNSDVAK 8
           +VNSD++K
Sbjct: 249 SVNSDISK 256
>NP_001159817
          Length = 246

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AVNSDVAK 8
           +VNSD++K
Sbjct: 140 SVNSDISK 147
>NP_001127812
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AVNSDVAKP 9
          +V SDV+KP
Sbjct: 52 SVASDVSKP 60
>NP_115678
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AVNSDVAKP 9
          +V SDV+KP
Sbjct: 52 SVASDVSKP 60
>XP_006715855
          Length = 122

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  AVNSDVAKP 9
          +V SDV+KP
Sbjct: 52 SVASDVSKP 60
>NP_001070671
          Length = 985

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 640 NTDAAKP 646
>NP_001269732
          Length = 886

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 541 NTDAAKP 547
>NP_001269731
          Length = 1017

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 672 NTDAAKP 678
>NP_001269730
          Length = 984

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 639 NTDAAKP 645
>NP_065831
          Length = 1392

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            A NS++ KP
Sbjct: 1060 AANSELVKP 1068
>NP_065705
          Length = 1050

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 705 NTDAAKP 711
>NP_065825
          Length = 1006

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   AVNSDVAK 8
           A N DVAK
Sbjct: 439 AANGDVAK 446
>XP_005247719
          Length = 919

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+D AKP
Sbjct: 574 NTDAAKP 580
>NP_065391
          Length = 252

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           NSD A+P
Sbjct: 159 NSDTAEP 165
>NP_976328
          Length = 911

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+DV KP
Sbjct: 685 NTDVEKP 691
>NP_055999
          Length = 1001

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           N+DV KP
Sbjct: 978 NADVQKP 984
>NP_001137854
          Length = 1127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    NSDVAKP 9
            N+DV KP
Sbjct: 1104 NADVQKP 1110
>NP_061874
          Length = 1706

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            AVN  V+KP
Sbjct: 1696 AVNFGVSKP 1704
>NP_001269786
          Length = 1694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            AVN  V+KP
Sbjct: 1684 AVNFGVSKP 1692
>NP_065868
          Length = 936

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VNSDVAKP 9
           VNS VA P
Sbjct: 622 VNSPVASP 629
>XP_005261680
          Length = 303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   NSDVAKP 9
           NSD A+P
Sbjct: 210 NSDTAEP 216
>XP_005247738
          Length = 615

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VNSDVAKP 9
           VNS VA P
Sbjct: 301 VNSPVASP 308
>XP_005247737
          Length = 661

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VNSDVAKP 9
           VNS VA P
Sbjct: 600 VNSPVASP 607
>XP_006713778
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VNSDVAKP 9
           VNS VA P
Sbjct: 609 VNSPVASP 616
>XP_005247601
          Length = 1484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            AVN  V+KP
Sbjct: 1474 AVNFGVSKP 1482
>XP_006713735
          Length = 1656

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            AVN  V+KP
Sbjct: 1646 AVNFGVSKP 1654
>XP_006713734
          Length = 1693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    AVNSDVAKP 9
            AVN  V+KP
Sbjct: 1683 AVNFGVSKP 1691
>XP_006713136
          Length = 1022

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3    NSDVAKP 9
            N+DV KP
Sbjct: 999  NADVQKP 1005
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.115    0.303 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 512,595
Number of extensions: 846
Number of successful extensions: 35
Number of sequences better than 10000.0: 35
Number of HSP's gapped: 35
Number of HSP's successfully gapped: 35
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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