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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005148                                                              18    1482
NP_009297                                                              18    1482
NP_054733                                                              16    5631
NP_001091952                                                           16    5631
NP_056136                                                              16    5631
NP_115731                                                              16    5631
XP_006722773                                                           16    5631
XP_005259893                                                           16    5631
XP_005259892                                                           16    5631
XP_005259891                                                           16    5631
XP_005259890                                                           16    5631
XP_006713408                                                           16    5631
NP_002560                                                              16    7355
NP_001276753                                                           16    7355
NP_001276752                                                           16    7355
NP_055031                                                              16    7355
NP_001136065                                                           16    7355
NP_001136066                                                           16    7355
NP_003631                                                              15    9605
NP_005024                                                              15    9605
NP_001029366                                                           15    9605
XP_005268838                                                           15    9605
XP_005268836                                                           15    9605
XP_005268834                                                           15    9605
XP_005268833                                                           15    9605
XP_005252342                                                           15    9605
NP_037376                                                              15    9605

>NP_005148
          Length = 1130

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVLEADKNL 9
            AVLEA KNL
Sbjct: 1055 AVLEAGKNL 1063



>NP_009297
          Length = 1149

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVLEADKNL 9
            AVLEA KNL
Sbjct: 1074 AVLEAGKNL 1082



>NP_054733
          Length = 2136

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   AVLEADKNL 9
           A LE D+NL
Sbjct: 491 AALETDENL 499



>NP_001091952
          Length = 650

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 210 EADKNL 215



>NP_056136
          Length = 634

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 194 EADKNL 199



>NP_115731
          Length = 652

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LEADKN 8
           LEADKN
Sbjct: 289 LEADKN 294



>XP_006722773
          Length = 512

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 210 EADKNL 215



>XP_005259893
          Length = 529

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 210 EADKNL 215



>XP_005259892
          Length = 564

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 210 EADKNL 215



>XP_005259891
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 194 EADKNL 199



>XP_005259890
          Length = 621

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EADKNL 9
           EADKNL
Sbjct: 210 EADKNL 215



>XP_006713408
          Length = 555

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   LEADKN 8
           LEADKN
Sbjct: 192 LEADKN 197



>NP_002560
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEADKNL 9
           LEA+KNL
Sbjct: 382 LEANKNL 388



>NP_001276753
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEADKNL 9
           LEA+KNL
Sbjct: 382 LEANKNL 388



>NP_001276752
          Length = 794

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LEADKNL 9
           LEA+KNL
Sbjct: 382 LEANKNL 388



>NP_055031
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VLEADKN 8
          +LEAD+N
Sbjct: 64 ILEADQN 70



>NP_001136065
          Length = 414

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VLEADKN 8
          +LEAD+N
Sbjct: 64 ILEADQN 70



>NP_001136066
          Length = 314

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  VLEADKN 8
          +LEAD+N
Sbjct: 64 ILEADQN 70



>NP_003631
          Length = 1332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AVLEADKN 8
            ++LEA KN
Sbjct: 1112 SILEAQKN 1119



>NP_005024
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLEADKN 8
           +LE DKN
Sbjct: 401 ILEPDKN 407



>NP_001029366
          Length = 456

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   VLEADKN 8
           +LE DKN
Sbjct: 418 ILEPDKN 424



>XP_005268838
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VLEADKNL 9
          VLE DK L
Sbjct: 48 VLEGDKQL 55



>XP_005268836
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VLEADKNL 9
          VLE DK L
Sbjct: 48 VLEGDKQL 55



>XP_005268834
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VLEADKNL 9
          VLE DK L
Sbjct: 77 VLEGDKQL 84



>XP_005268833
          Length = 507

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VLEADKNL 9
          VLE DK L
Sbjct: 77 VLEGDKQL 84



>XP_005252342
          Length = 1218

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    AVLEADKN 8
            ++LEA KN
Sbjct: 998  SILEAQKN 1005



>NP_037376
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  VLEADKNL 9
          VLE DK L
Sbjct: 48 VLEGDKQL 55



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.126    0.304 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 497,830
Number of extensions: 924
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)