Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065136                                                              18    1935
NP_982354                                                              18    1935
XP_005266465                                                           18    1935
XP_005266464                                                           18    1935
XP_005266463                                                           18    1935
NP_006197                                                              17    2528
XP_005265800                                                           17    2528
XP_006714103                                                           17    2528
XP_006714102                                                           17    2528
NP_996879                                                              17    3301
XP_006710494                                                           17    3301
XP_006710492                                                           17    3301
XP_006710491                                                           17    3301
XP_006710490                                                           17    3301
XP_006710489                                                           17    3301
NP_689777                                                              17    4312
NP_060360                                                              17    4312
XP_005250858                                                           17    4312
XP_005250857                                                           17    4312
NP_065813                                                              16    7355
NP_055127                                                              16    7355
NP_660348                                                              16    7355
XP_005262449                                                           16    7355
XP_006724802                                                           16    7355
XP_006724801                                                           16    7355
XP_005262448                                                           16    7355
XP_005262447                                                           16    7355
XP_005269099                                                           16    7355
XP_006718869                                                           16    7355
NP_065979                                                              15    9605
NP_001276065                                                           15    9605
NP_004778                                                              15    9605
NP_001276064                                                           15    9605
NP_065934                                                              15    9605
NP_061897                                                              15    9605
XP_005260080                                                           15    9605
XP_005248271                                                           15    9605
XP_006714049                                                           15    9605
XP_005248268                                                           15    9605
XP_005264436                                                           15    9605
XP_006712098                                                           15    9605
XP_006712097                                                           15    9605
NP_006387                                                              15    9605
>NP_065136
          Length = 1203

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VACMRVDS 9
          +AC+R+DS
Sbjct: 13 IACLRLDS 20
>NP_982354
          Length = 1237

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VACMRVDS 9
          +AC+R+DS
Sbjct: 13 IACLRLDS 20
>XP_005266465
          Length = 1019

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VACMRVDS 9
          +AC+R+DS
Sbjct: 13 IACLRLDS 20
>XP_005266464
          Length = 1161

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VACMRVDS 9
          +AC+R+DS
Sbjct: 13 IACLRLDS 20
>XP_005266463
          Length = 1195

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  VACMRVDS 9
          +AC+R+DS
Sbjct: 13 IACLRLDS 20
>NP_006197
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVACMRVDS 9
           AVA MRVDS
Sbjct: 976 AVARMRVDS 984
>XP_005265800
          Length = 1089

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   AVACMRVDS 9
           AVA MRVDS
Sbjct: 976 AVARMRVDS 984
>XP_006714103
          Length = 1111

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVACMRVDS 9
            AVA MRVDS
Sbjct: 998  AVARMRVDS 1006
>XP_006714102
          Length = 1114

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    AVACMRVDS 9
            AVA MRVDS
Sbjct: 1001 AVARMRVDS 1009
>NP_996879
          Length = 2223

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VACMRVDS 9
            V CMRV+S
Sbjct: 1488 VKCMRVES 1495
>XP_006710494
          Length = 1152

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           V CMRV+S
Sbjct: 417 VKCMRVES 424
>XP_006710492
          Length = 1351

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           V CMRV+S
Sbjct: 616 VKCMRVES 623
>XP_006710491
          Length = 1496

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           V CMRV+S
Sbjct: 761 VKCMRVES 768
>XP_006710490
          Length = 2158

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VACMRVDS 9
            V CMRV+S
Sbjct: 1423 VKCMRVES 1430
>XP_006710489
          Length = 2185

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2    VACMRVDS 9
            V CMRV+S
Sbjct: 1450 VKCMRVES 1457
>NP_689777
          Length = 3997

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ACMRVDS 9
            AC RVDS
Sbjct: 2438 ACTRVDS 2444
>NP_060360
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ACMRVDS 9
            AC RVDS
Sbjct: 2463 ACTRVDS 2469
>XP_005250858
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ACMRVDS 9
            AC RVDS
Sbjct: 2463 ACTRVDS 2469
>XP_005250857
          Length = 4022

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3    ACMRVDS 9
            AC RVDS
Sbjct: 2463 ACTRVDS 2469
>NP_065813
          Length = 1085

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 2   VACMRVDS 9
           ++C+R+D+
Sbjct: 601 ISCIRIDN 608
>NP_055127
          Length = 327

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   CMRVDS 9
           CMR DS
Sbjct: 297 CMRADS 302
>NP_660348
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_005262449
          Length = 226

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_006724802
          Length = 237

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_006724801
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_005262448
          Length = 256

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_005262447
          Length = 269

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  AVACMRV 7
          AVAC+R+
Sbjct: 73 AVACLRL 79
>XP_005269099
          Length = 1062

 Score = 15.8 bits (29), Expect =  7355
 Identities = 3/8 (37%), Positives = 8/8 (100%)

Query: 2   VACMRVDS 9
           ++C+R+D+
Sbjct: 578 ISCIRIDN 585
>XP_006718869
          Length = 270

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 4   CMRVDS 9
           CMR DS
Sbjct: 240 CMRADS 245
>NP_065979
          Length = 1215

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ACMRVDS 9
           AC RVD+
Sbjct: 569 ACARVDA 575
>NP_001276065
          Length = 1521

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 384 INCLRVDA 391
>NP_004778
          Length = 1529

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 384 INCLRVDA 391
>NP_001276064
          Length = 1525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 388 INCLRVDA 395
>NP_065934
          Length = 1185

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ACMRVDS 9
           AC RVD+
Sbjct: 542 ACARVDA 548
>NP_061897
          Length = 2071

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AVACMR 6
            AVAC+R
Sbjct: 1086 AVACLR 1091
>XP_005260080
          Length = 1106

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   ACMRVDS 9
           AC RVD+
Sbjct: 467 ACARVDT 473
>XP_005248271
          Length = 1404

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 259 INCLRVDA 266
>XP_006714049
          Length = 1509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 388 INCLRVDA 395
>XP_005248268
          Length = 1533

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   VACMRVDS 9
           + C+RVD+
Sbjct: 388 INCLRVDA 395
>XP_005264436
          Length = 1881

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AVACMR 6
            AVAC+R
Sbjct: 1086 AVACLR 1091
>XP_006712098
          Length = 2059

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AVACMR 6
            AVAC+R
Sbjct: 1074 AVACLR 1079
>XP_006712097
          Length = 2070

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1    AVACMR 6
            AVAC+R
Sbjct: 1086 AVACLR 1091
>NP_006387
          Length = 199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2  VACMRVDS 9
          VAC  +DS
Sbjct: 71 VACQELDS 78
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.126    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 527,171
Number of extensions: 515
Number of successful extensions: 43
Number of sequences better than 10000.0: 43
Number of HSP's gapped: 43
Number of HSP's successfully gapped: 43
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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