Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006197                                                              20     390
XP_005265800                                                           20     390
XP_006714103                                                           20     390
XP_006714102                                                           20     390
NP_002010                                                              18    1935
NP_002600                                                              17    4312
NP_005537                                                              17    4312
XP_005268521                                                           17    4312
NP_037361                                                              16    5631
NP_863651                                                              16    5631
NP_001151                                                              16    5631
NP_863658                                                              16    5631
NP_001035022                                                           16    5631
NP_000617                                                              16    5631
NP_570138                                                              16    5631
NP_689618                                                              16    7355
NP_001094387                                                           16    7355
XP_005268726                                                           16    7355
XP_006719313                                                           16    7355
XP_006714593                                                           16    7355
XP_005248487                                                           16    7355
NP_002218                                                              15    9605
NP_001153839                                                           15    9605
NP_443714                                                              15    9605
NP_001092003                                                           15    9605
NP_443713                                                              15    9605
NP_002106                                                              15    9605
XP_006721381                                                           15    9605
XP_006721380                                                           15    9605
XP_006721379                                                           15    9605
XP_006721378                                                           15    9605
XP_006721377                                                           15    9605
XP_006721376                                                           15    9605
XP_006721375                                                           15    9605
XP_006721374                                                           15    9605
XP_006721373                                                           15    9605
XP_006721372                                                           15    9605
XP_006721371                                                           15    9605
XP_005268438                                                           15    9605
XP_005270898                                                           15    9605
XP_006710687                                                           15    9605
NP_001025040                                                           15    9605
>NP_006197
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ATSEVYQIM 9
           ATSEVY+IM
Sbjct: 921 ATSEVYEIM 929
>XP_005265800
          Length = 1089

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ATSEVYQIM 9
           ATSEVY+IM
Sbjct: 921 ATSEVYEIM 929
>XP_006714103
          Length = 1111

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ATSEVYQIM 9
           ATSEVY+IM
Sbjct: 943 ATSEVYEIM 951
>XP_006714102
          Length = 1114

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ATSEVYQIM 9
           ATSEVY+IM
Sbjct: 946 ATSEVYEIM 954
>NP_002010
          Length = 1338

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    ATSEVYQIM 9
            +T E+YQIM
Sbjct: 1125 STPEIYQIM 1133
>NP_002600
          Length = 1106

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           A+ E+Y+IM
Sbjct: 929 ASDEIYEIM 937
>NP_005537
          Length = 620

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           A++ VYQIM
Sbjct: 583 ASTHVYQIM 591
>XP_005268521
          Length = 1042

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           A+ E+Y+IM
Sbjct: 865 ASDEIYEIM 873
>NP_037361
          Length = 1237

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 562 TSEVYQ 567
>NP_863651
          Length = 1248

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 573 TSEVYQ 578
>NP_001151
          Length = 1194

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 562 TSEVYQ 567
>NP_863658
          Length = 1205

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 573 TSEVYQ 578
>NP_001035022
          Length = 230

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 130 TSEVYQ 135
>NP_000617
          Length = 229

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   TSEVYQ 7
           TSEVYQ
Sbjct: 129 TSEVYQ 134
>NP_570138
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   ATSEVYQIM 9
           A S VYQ+M
Sbjct: 176 AQSRVYQLM 184
>NP_689618
          Length = 988

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           AT+E+YQ +
Sbjct: 375 ATTELYQYL 383
>NP_001094387
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSEV+Q+
Sbjct: 224 TSEVFQV 230
>XP_005268726
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSEV+Q+
Sbjct: 224 TSEVFQV 230
>XP_006719313
          Length = 259

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSEV+Q+
Sbjct: 235 TSEVFQV 241
>XP_006714593
          Length = 696

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           AT+E+YQ +
Sbjct: 375 ATTELYQYL 383
>XP_005248487
          Length = 947

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ATSEVYQIM 9
           AT+E+YQ +
Sbjct: 375 ATTELYQYL 383
>NP_002218
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EVYQIM 9
            EVYQ+M
Sbjct: 1123 EVYQLM 1128
>NP_001153839
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>NP_443714
          Length = 360

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 242 TSEIHQI 248
>NP_001092003
          Length = 283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>NP_443713
          Length = 272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>NP_002106
          Length = 923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2   TSEVYQI 8
           TSE+Y I
Sbjct: 562 TSEIYSI 568
>XP_006721381
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721380
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721379
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721378
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721377
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721376
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 171 TSEIHQI 177
>XP_006721375
          Length = 313

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 195 TSEIHQI 201
>XP_006721374
          Length = 323

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 205 TSEIHQI 211
>XP_006721373
          Length = 343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 242 TSEIHQI 248
>XP_006721372
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 236 TSEIHQI 242
>XP_006721371
          Length = 354

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TSE++QI
Sbjct: 242 TSEIHQI 248
>XP_005268438
          Length = 157

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TS+VY+I
Sbjct: 140 TSQVYEI 146
>XP_005270898
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EVYQIM 9
            EVYQ+M
Sbjct: 1123 EVYQLM 1128
>XP_006710687
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4    EVYQIM 9
            EVYQ+M
Sbjct: 1123 EVYQLM 1128
>NP_001025040
          Length = 177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   TSEVYQI 8
           TS+VY+I
Sbjct: 160 TSQVYEI 166
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.118    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 367,606
Number of extensions: 381
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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