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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245210                                                           19     869
NP_000242                                                              19     869
XP_005264389                                                           19     869
NP_006614                                                              16    5631
NP_065750                                                              16    7355
NP_000867                                                              16    7355
XP_005245421                                                           16    7355
XP_006711532                                                           16    7355
XP_005245420                                                           16    7355
NP_000407                                                              15    9605
XP_006715534                                                           15    9605
XP_006715533                                                           15    9605

>NP_001245210
          Length = 868

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASIIRSATK 9
           ASI+RSATK
Sbjct: 667 ASILRSATK 675



>NP_000242
          Length = 934

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASIIRSATK 9
           ASI+RSATK
Sbjct: 733 ASILRSATK 741



>XP_005264389
          Length = 921

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   ASIIRSATK 9
           ASI+RSATK
Sbjct: 733 ASILRSATK 741



>NP_006614
          Length = 533

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  IIRSATK 9
          I+RSATK
Sbjct: 52 IVRSATK 58



>NP_065750
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S+IRSAT
Sbjct: 246 SVIRSAT 252



>NP_000867
          Length = 2491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SIIRSATK 9
           S++RSAT+
Sbjct: 99  SVLRSATR 106



>XP_005245421
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S+IRSAT
Sbjct: 246 SVIRSAT 252



>XP_006711532
          Length = 593

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S+IRSAT
Sbjct: 246 SVIRSAT 252



>XP_005245420
          Length = 599

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S+IRSAT
Sbjct: 252 SVIRSAT 258



>NP_000407
          Length = 489

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S++RSAT
Sbjct: 289 SVVRSAT 295



>XP_006715534
          Length = 448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S++RSAT
Sbjct: 248 SVVRSAT 254



>XP_006715533
          Length = 479

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SIIRSAT 8
           S++RSAT
Sbjct: 279 SVVRSAT 285



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.114    0.251 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 187,444
Number of extensions: 126
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)