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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005219                                                              17    2528
NP_001677                                                              16    7355
NP_775764                                                              15    9605
NP_149061                                                              15    9605
NP_001010906                                                           15    9605
XP_005273560                                                           15    9605
XP_005273559                                                           15    9605
XP_005273558                                                           15    9605
NP_001073875                                                           15    9605
NP_078799                                                              15    9605
NP_060299                                                              15    9605

>NP_005219
          Length = 1210

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1   ASEISSIL 8
           ASEISSIL
Sbjct: 920 ASEISSIL 927



>NP_001677
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SEISSILG 9
           SE+S++LG
Sbjct: 316 SEVSALLG 323



>NP_775764
          Length = 773

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  ASEISSILG 9
          A  ISS+LG
Sbjct: 58 AENISSLLG 66



>NP_149061
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   ASEISSILG 9
           A E+S++LG
Sbjct: 100 AQELSALLG 108



>NP_001010906
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EISSILG 9
           EIS+ILG
Sbjct: 636 EISAILG 642



>XP_005273560
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EISSILG 9
           EIS+ILG
Sbjct: 512 EISAILG 518



>XP_005273559
          Length = 701

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EISSILG 9
           EIS+ILG
Sbjct: 541 EISAILG 547



>XP_005273558
          Length = 796

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   EISSILG 9
           EIS+ILG
Sbjct: 636 EISAILG 642



>NP_001073875
          Length = 269

 Score = 15.4 bits (28), Expect =  9605
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1   ASEISSIL 8
           A EISSIL
Sbjct: 185 APEISSIL 192



>NP_078799
          Length = 775

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SEISSILG 9
           SE+S +LG
Sbjct: 371 SEVSELLG 378



>NP_060299
          Length = 393

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  ASEISSILG 9
          A E+S++LG
Sbjct: 70 AQELSALLG 78



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.300    0.121    0.273 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 318,442
Number of extensions: 433
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)