Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036213                                                              16    5631
XP_006714638                                                           16    5631
XP_006714637                                                           16    5631
NP_005337                                                              16    7355
NP_005336                                                              16    7355
NP_000855                                                              16    7355
NP_620706                                                              15    9605
NP_003066                                                              15    9605
NP_001123892                                                           15    9605
NP_443148                                                              15    9605
NP_005112                                                              15    9605
XP_006721725                                                           15    9605
XP_006721723                                                           15    9605
XP_005269161                                                           15    9605
XP_005269160                                                           15    9605
XP_005269159                                                           15    9605
XP_005269158                                                           15    9605
>NP_036213
          Length = 640

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AQRKRIS 7
           AQRKR+S
Sbjct: 574 AQRKRLS 580
>XP_006714638
          Length = 585

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AQRKRIS 7
           AQRKR+S
Sbjct: 519 AQRKRLS 525
>XP_006714637
          Length = 589

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   AQRKRIS 7
           AQRKR+S
Sbjct: 574 AQRKRLS 580
>NP_005337
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRKRISAR 9
           QR+R+SA+
Sbjct: 532 QRERVSAK 539
>NP_005336
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   QRKRISAR 9
           QR+R+SA+
Sbjct: 532 QRERVSAK 539
>NP_000855
          Length = 377

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   QRKRISA 8
           +RKRISA
Sbjct: 287 ERKRISA 293
>NP_620706
          Length = 1130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>NP_003066
          Length = 1214

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>NP_001123892
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>NP_443148
          Length = 679

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AQRKRISA 8
           AQR+R+S+
Sbjct: 654 AQRRRLSS 661
>NP_005112
          Length = 2174

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    AQRKRISAR 9
            A+RK+ SAR
Sbjct: 1828 ARRKKSSAR 1836
>XP_006721725
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AQRKRISA 8
           AQR+R+S+
Sbjct: 588 AQRRRLSS 595
>XP_006721723
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   AQRKRISA 8
           AQR+R+S+
Sbjct: 655 AQRRRLSS 662
>XP_005269161
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>XP_005269160
          Length = 1213

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>XP_005269159
          Length = 1244

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
>XP_005269158
          Length = 1245

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   AQRKRISAR 9
           ++RK+ISA+
Sbjct: 267 SRRKKISAK 275
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.124    0.296 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 380,877
Number of extensions: 506
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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