Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_443179                                                              19     869
NP_001027545                                                           16    7355
XP_005259285                                                           16    7355
XP_006723432                                                           16    7355
XP_006723431                                                           16    7355
XP_005259284                                                           16    7355
XP_006723430                                                           16    7355
XP_005259283                                                           16    7355
XP_005259282                                                           16    7355
XP_005259281                                                           16    7355
NP_001027544                                                           16    7355
NP_056197                                                              15    9605
NP_056526                                                              15    9605
XP_006723243                                                           15    9605
XP_005258890                                                           15    9605
XP_005267560                                                           15    9605
XP_005267559                                                           15    9605
NP_005504                                                              15    9605
>NP_443179
          Length = 2170

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   ANLRESTIE 9
           ANLREST E
Sbjct: 551 ANLRESTTE 559
>NP_001027545
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_005259285
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_006723432
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_006723431
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_005259284
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_006723430
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_005259283
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_005259282
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>XP_005259281
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>NP_001027544
          Length = 803

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   NLRESTIE 9
           N+REST E
Sbjct: 791 NIRESTQE 798
>NP_056197
          Length = 2610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NLRESTIE 9
            N RE+T+E
Sbjct: 2100 NRREATVE 2107
>NP_056526
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ANLRESTIE 9
           AN+ E T+E
Sbjct: 111 ANITEQTLE 119
>XP_006723243
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ANLRESTIE 9
           AN+ E T+E
Sbjct: 111 ANITEQTLE 119
>XP_005258890
          Length = 1560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   ANLRESTIE 9
           AN+ E T+E
Sbjct: 111 ANITEQTLE 119
>XP_005267560
          Length = 2563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NLRESTIE 9
            N RE+T+E
Sbjct: 2053 NRREATVE 2060
>XP_005267559
          Length = 2610

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    NLRESTIE 9
            N RE+T+E
Sbjct: 2100 NRREATVE 2107
>NP_005504
          Length = 439

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LRESTIE 9
           LREST++
Sbjct: 281 LRESTVQ 287
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.116    0.267 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 405,192
Number of extensions: 413
Number of successful extensions: 18
Number of sequences better than 10000.0: 18
Number of HSP's gapped: 18
Number of HSP's successfully gapped: 18
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.2 bits)
S2: 28 (15.4 bits)

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